4603c27 | IMR90c01 | IMR90-4 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
20 | 1553 | 1514 | 1 | 0 | 1517 | 1519 | 1 | 0.02 | 1552 | 1510 | 0.91 | 0.35 |
10 | 1520 | 1564 | 0.78 | 0.65 | 1555 | 2021 | 0.66 | 0.60 | 2020 | 1516 | 0.87 | 0.60 |
2012 | 1564 | 0.74 | 0.56 | 2042 | 2021 | 0.77 | 2022 | 1516 | 0.80 | 0.62 | ||
2041 | 1564 | 0.57 | 2050 | 2021 | 0.64 | 0.90 | 2055 | 1516 | 0.90 | 0.55 | ||
2044 | 1564 | 0.72 | 0.60 | 2053 | 2021 | 0.64 | 0.57 | 2020 | 1556 | 0.88 | 0.59 | |
1520 | 2047 | 0.77 | 0.70 | 1555 | 2048 | 0.63 | 2022 | 1556 | 0.81 | 0.62 | ||
2012 | 2047 | 0.73 | 0.62 | 2042 | 2048 | 0.79 | 2055 | 1556 | 0.92 | 0.55 | ||
2041 | 2047 | 0.80 | 0.62 | 2050 | 2048 | 0.65 | 0.92 | 2020 | 2010 | 0.89 | 0.59 | |
2044 | 2047 | 0.65 | 2053 | 2048 | 0.65 | 0.60 | 2022 | 2010 | 0.82 | 0.61 | ||
2055 | 2010 | 0.54 | ||||||||||
2020 | 2013 | 0.86 | 0.60 | |||||||||
2022 | 2013 | 0.62 | ||||||||||
2055 | 2013 | 0.89 | 0.56 | |||||||||
2020 | 2014 | 0.87 | 0.63 | |||||||||
2022 | 2014 | 0.80 | 0.65 | |||||||||
2055 | 2014 | 0.91 | 0.58 | |||||||||
Component | Concentration | |
---|---|---|
NIM | NPM | |
Advanced DMEM/F12 : Neurobasal™ | 1:1 | 1:1 |
B-27™ Supplement | 2 % (v/v) | 2 % (v/v) |
N-2 Supplement | 1 % (v/v) | 1 % (v/v) |
GlutaMax™ | 1 % (v/v) | 1 % (v/v) |
LIF | 10 ng/ml | 10 ng/ml |
CHIR99021 | 4 μM | 3 μM |
SB431542 | 3 μM | 2 μM |
Compound E | 0.1 μM | - |
BSA | 0.0005 % (w/v) | 0.0005 % (w/v) |
4603c27 | IMR90c01 | IMR90-4 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
20 | 1553 | 1514 | 1 | 0 | 1517 | 1519 | 1 | 0.02 | 1552 | 1510 | 0.91 | 0.35 |
20 | 1520 | 1564 | 0.88 | 0.56 | 1555 | 2021 | 0.78 | 0.64 | 2020 | 1516 | 0.87 | 0.50 |
2012 | 1564 | 0.87 | 0.77 | 2042 | 2021 | 0.74 | 0.56 | 2022 | 1516 | 0.96 | 0.45 | |
2041 | 1564 | 0.94 | 0.22 | 2050 | 2021 | 0.57 | 2055 | 1516 | 0.95 | 0.42 | ||
2044 | 1564 | 0.15 | 2053 | 2021 | 0.72 | 0.60 | 2020 | 1556 | 0.53 | |||
1520 | 2047 | 0.86 | 0.55 | 1555 | 2048 | 0.77 | 0.77 | 2022 | 1556 | 0.93 | 0.47 | |
2012 | 2047 | 0.79 | 2042 | 2048 | 0.73 | 0.73 | 2055 | 1556 | 0.91 | 0.44 | ||
2041 | 2047 | 0.93 | 0.16 | 2050 | 2048 | 0.81 | 0.80 | 2020 | 2010 | 0.86 | 0.52 | |
2044 | 2047 | 0.82 | 2053 | 2048 | 0.71 | 2022 | 2010 | 0.46 | ||||
2055 | 2010 | 0.96 | 0.43 | |||||||||
2020 | 2013 | 0.83 | 0.55 | |||||||||
2022 | 2013 | 0.93 | 0.48 | |||||||||
2055 | 2013 | 0.92 | 0.46 | |||||||||
2020 | 2014 | 0.84 | 0.58 | |||||||||
2022 | 2014 | 0.95 | 0.51 | |||||||||
2055 | 2014 | 0.93 | 0.48 | |||||||||
Component | Concentration |
---|---|
Astrocyte proliferation medium | |
Neurobasal™ | |
FBS | 10 % (v/v) |
GlutaMax™ | 1.5 % (v/v) |
Pen/Strep | 0.2 % (v/v) |
Gentamicin | 0.04 % (v/v) |
Astro-NDM | |
DMEM/F12 | |
Apotransferrin | 0.0095 % (w/v) |
Ascorbic acid | 300 μM |
cAMP | 300 nM |
Glucose monohydrate | 33.3 mM |
GlutaMax™ | 1 % (v/v) |
Heparin | 0.0004 % (v/v) |
HEPES | 5 mM |
Insulin, human | 0.0025 % (w/v) |
NaHCO3 | 3 mM |
Sodium selenite | 30 nM |
Progesterone | 20 nM |
Putrescine | 60 nM |
Pen/Strep | 0.2 % (v/v) |
Gentamicin | 0.04 % (v/v) |