1. bookVolumen 58 (2014): Heft 1 (June 2014)
19 Jun 2012
2 Hefte pro Jahr
access type Uneingeschränkter Zugang

Body-Surface Compounds in Buckfast and Caucasian Honey Bee Workers (Apis Mellifera)

Online veröffentlicht: 27 May 2014
Volumen & Heft: Volumen 58 (2014) - Heft 1 (June 2014)
Seitenbereich: 5 - 15
Eingereicht: 26 Nov 2012
Akzeptiert: 21 Feb 2014
19 Jun 2012
2 Hefte pro Jahr

Body-surface chemical compounds were studied in 1-day-old nest workers and foragers both in Buckfast and Caucasian bees. The workers of these two age-castes were sampled twice in each of two consecutive years. Body-surface lipids were determined by means of gas chromatography, with a GCQ mass spectrometer. Protein concentrations and activities on the body surface were examined in bee cuticle rinsings obtained from worker bees according to the methods of Lowry, of Anson, and of Lee and Lin. Protease and protease inhibitor activities were determined. Polyacrylamide gel electrophoresis was performed. Caucasian bees, particularly foragers, had more lipids, but Buckfast bees (two age-castes) had more proteins on their body surfaces. A total of 17 alkane types (C17 - C33), 13 alkene types (C21 - C33), 21 esters (C12 - C32), and a phenol (C14) were detected in both races. Alkene C33 was detected only in Caucasian bees. More alkanes, esters, and phenols were found in Caucasian 1-day-old nest workers and foragers than in these age-castes of Buckfast bees. The protein concentration and protease inhibitor activities were lower in Caucasian bees that had higher protease activities. These values corresponded with specific numbers and widths of the electrophoretic bands.

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