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Ziziphora hispanica L. (Lamiaceae) is a medicinal aromatic plant which is native to Algeria in semi-arid regions, Morocco and southern Spain. Despite the medicinal and economic interests as well as the uncertain future that this species may encounter in its natural habitats, various aspects of the biology of its seeds have not been recognized up to date. To fulfill these gaps, seed germination has been studied under the effect of different incubation temperatures, and under the influence of defer-ential concentrations of water stress and saline stress. The seeds were first incubated at different temperatures (15, 20 and 25°C). Then, at 20°C, germination studies were carried out at various sodium chlo-ride concentrations (0, 25, 50, 75 and 100 Mmol /l) and various solutions of polyethylene glycol (PEG6000) (0, -0.2, -0.4, and-0.6 Mpa). The results showed that Z. hispanica seeds were not dormant, and the optimal temperature of germination was 20°C, at which the maximum final germination percentage occurred (94%). A high negative correlation was obtained between the germination percentage and the various concentrations of NaCl. In comparison to other plant species that share its habitats, the results of the water stress impact tests indicate that Z. hispanica is very sensitive to water stress, indicating difficulties in germinating in semi-arid and arid regions and that climate change may restrict its natural habitats. The results of this study can serve as guidelines for propagation protocols of this species to support its conservation and cultivation.
