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Redescription of Laimydorus siddiqii Baqri & Jana, 1982 (Dorylaimida: Dorylaimidae) with its first molecular study

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24. Mai 2025

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COVER HERUNTERLADEN

Fig. 1.

Laimydorus siddiqii (Female) from Uttarakhand, India (light microscopy). A: Anterior region with double guiding ring (arrowed); B: Pharyngeal expansion; C: Entire female; D: Posterior region; E: Prerectum; F: Tail region; G: Genital system; H: Cardiac region; I: Anterior ovary J: Vagina (arrowed); K: Posterior ovary. Scale bars: (A,G,H,I,K), 10 μm; (B,D,E,F) 20 μm; (C)100 μm.
Laimydorus siddiqii (Female) from Uttarakhand, India (light microscopy). A: Anterior region with double guiding ring (arrowed); B: Pharyngeal expansion; C: Entire female; D: Posterior region; E: Prerectum; F: Tail region; G: Genital system; H: Cardiac region; I: Anterior ovary J: Vagina (arrowed); K: Posterior ovary. Scale bars: (A,G,H,I,K), 10 μm; (B,D,E,F) 20 μm; (C)100 μm.

Fig. 2.

Laimydorus siddiqii (Male) from Uttarakhand, India (light microscopy). A: Anterior body region; B: Pharyngeal expansion; C: Pharyngointestinal junction; D: Entire male; E: Anterior region with double guiding ring; F: Spicules; G: Posterior body region; Scale bars: (A,E,C), 10 μm; (D) 100 μm; (B,F,G), 20 μm.
Laimydorus siddiqii (Male) from Uttarakhand, India (light microscopy). A: Anterior body region; B: Pharyngeal expansion; C: Pharyngointestinal junction; D: Entire male; E: Anterior region with double guiding ring; F: Spicules; G: Posterior body region; Scale bars: (A,E,C), 10 μm; (D) 100 μm; (B,F,G), 20 μm.

Fig. 3.

Phylogenetic tree based on D2-D3 expansion segments of the 28S rRNA gene sequences of the nematodes isolated in this study and several related species. Phylogenetic relationships based on the nucleotide sequences of the D2-D3 expansion segments of the 28S rRNA gene were inferred by using the Maximum Likelihood method based on the General Time Reversible Model. The tree with the highest log likelihood is shown. The percentage of trees in which the associated taxa clustered together is shown next to the branches. A discrete Gamma distribution was used to model evolutionary rate differences among sites. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. NCBI accession numbers of the sequences used for the analyses are shown.
Phylogenetic tree based on D2-D3 expansion segments of the 28S rRNA gene sequences of the nematodes isolated in this study and several related species. Phylogenetic relationships based on the nucleotide sequences of the D2-D3 expansion segments of the 28S rRNA gene were inferred by using the Maximum Likelihood method based on the General Time Reversible Model. The tree with the highest log likelihood is shown. The percentage of trees in which the associated taxa clustered together is shown next to the branches. A discrete Gamma distribution was used to model evolutionary rate differences among sites. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. NCBI accession numbers of the sequences used for the analyses are shown.

Fig. 4.

Phylogenetic tree based on 18S rRNA sequences of the nematodes isolated in this study and several related species. Phylogenetic relationships based on 18S rRNA gene sequences were inferred by using the Maximum Likelihood method based on the Tamura 3-parameter model. The tree with the highest log likelihood is shown. The percentage of trees in which the associated taxa clustered together is shown next to the branches. A discrete Gamma distribution was used to model evolutionary rate differences among sites. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. NCBI accession numbers of the sequences used for the analyses are shown.
Phylogenetic tree based on 18S rRNA sequences of the nematodes isolated in this study and several related species. Phylogenetic relationships based on 18S rRNA gene sequences were inferred by using the Maximum Likelihood method based on the Tamura 3-parameter model. The tree with the highest log likelihood is shown. The percentage of trees in which the associated taxa clustered together is shown next to the branches. A discrete Gamma distribution was used to model evolutionary rate differences among sites. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. NCBI accession numbers of the sequences used for the analyses are shown.

Morphometry of Laimydorus siddiqii Baqri and Jana, 1982 from Uttarakhand, India_ All the measurements are in μm (except n, ratio and percentage) and are in the form of mean ± standard deviation (range)_

Characters Females Males
N 10 6
Total body length (L) 2913 ± 162 (2601 – 3100) 2710 ± 312 (2393 – 3108)
a (L/BD) 38 ± 4.1 (31 – 42) 35 ± 2.4 (33 – 39)
b (L/NL) 5.1 ± 0.2 (4.8 – 5.2) 4.6 ± 0.4 (4.2 – 5.2)
c (L/TL) 12.1 ± 0.8 (11 – 14) 110.14 ± 16.6 (94 – 141)
c' (T/ABD) 7.9 ± 0.6 (6.5 – 8.9) 0.8 ± 0.1 (0.7 – 1)
V % (AV/L) ×100 48 ± 1.9 (45 – 52)
Lip region width 15.7 ± 1.2 (14 – 18) 15.2 ± 0.4 (15 – 16)
Lip region height 5.7 ± 0.4 (5.1 – 6) 5.9 ± 05 (5 – 5.6)
Amphid aperture width 8.2 ± 1.3 (7 – 10) 6.6 ± 0.57 (6 – 7)
G1((AUL + AOVL+ AOL)/L×100) 22.4 ± 1.5 (20.6 – 25.6)
G2 ((PUL+ POVL+ POL/L) × 100) 21.4 ± 1.9 (19.3 – 23.6)
Cuticle at anterior region 1.85 ± 0.2 (1.5 – 2) 1.9 ± 0.2 (1.4 – 2)
Cuticle at mid region 2 ± 0.35 (1.5 – 2.5) 1.2 ± 0.2 (1 – 1.5)
Cuticle at tail region 2.52 ± 0.4 (2 – 3) 2.9 ± 0.2 (2.5 – 3)
Odontostyle length 31.5 ± 0.8 (30 – 33) 31.9 ± 0.6 (31 – 33)
Odontostyle length-dorsal side 21.5 ± 2.0 (19 – 26) 21.7 ± 1 (20 – 23)
Odontostyle length-ventral side 30.7 ± 1.4 (29 – 34) 30.6 ± 0.8 (30 – 32)
Odontostyle width 3.6 ± 0.6 (3.1 – 5.2) 3.7 ± 0.5 (3 – 4)
Odontostyle aperture length 10.5 ± 0.9 (9 – 12) 10.2 ± 1.1 (9 – 12)
Odontophore length 35.8 ± 2.2 (33 – 40) 36.4 ± 0.8 (35 – 37)
Distance guiding ring-anterior end 19.3 ± 0.7 (18 – 20) 18.2 ± 0.7 (17 – 19)
Distance nerve ring-anterior end 170.6 ± 9.6 (155 – 185) 182.3 ± 27.5 (155 – 210)
Neck length (NL) 583 ± 31.7 (532 – 652) 581.6 ± 12.9 (563 – 598)
Pharyngeal expansion length 283 ± 21.2 (244 – 306) 282 ± 14.8 (255 – 299)
Pharyngeal expansion width 38 ± 2.8 (35 – 44) 46.5 ± 3.1 (44 – 51)
Body diameter at neck base 77 ± 8.2 (65.2 – 86) 67.6 ± 2.6 (63 – 70)
Cardia length 24.9 ± 3.1 (19 – 30) 24.1 ± 3.1 (21 – 30)
Cardia width 19.4 ± 1.9 (18 – 24) 16.3 ± 1.6 (14 – 18)
Body diameter at vulva 78.1 ± 8.1 (65 – 86)
Anterior uterus length (AUL) 272.3 ± 11.5 (250 – 290)
Anterior oviduct length (AOVL) 190.4 ± 0.5 (190 – 191)
Anterior ovary length (AOL 187.8 ± 3.5 (183 – 192)
Posterior uterus length (PUL) 261.6 ± 18 (225 – 292)
Posterior oviduct length (POVL) 173.5 ± 7.7 (160 – 182)
Posterior ovary length (POL 188.7 ± 5.3 (180 – 198)
Vagina length 21.5 ± 1.9 (20 – 26)
Distance vulva-anterior end (AV) 1407 ± 93.2 (1241 – 1558)
Prerectum length 188 ± 16.9 (167 – 210) 333 ± 61.9 (290 – 404)
Rectum length 42.5 ± 5.2 (34 – 48) 65.2 ± 10.10 (53 – 80)
Anal body diameter (ABD) 30.4 ± 1.3 (28 – 32) 31.5 ± 2.6 (28 – 35)
Tail length (TL) 213 ± 27.8 (183 – 268) 26.5 ± 1.3 (25 – 28)
Vulva to anus 1272 ± 99.2 (1124 – 1410)
Body diameter (BD) 78.1 ± 8.1 (65 – 86) 72.1 ± 5.1 (63 – 78)
Spicule length 59.5 ± 2.8 (56 – 63)
Max. width of spicule 10.0 ± 0.8 (9.0 – 11.0)
Distance of hump from anterior end of spicule 18.5 ± 0.7 (18 – 19)
Spicule width at posterior tip 5.2 ± 0.6 (4.7 – 6)
Ventromedian supplements 27.3 ± 0.8 (26 – 28)
Distance between supplements 1.6 ± 0.2 (1.5 – 2.0)
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Biologie, Zoologie, Ökologie, Biologie, andere, Medizin, Klinische Medizin, Mikrobiologie, Virologie und Infektionsepidemiologie