PCR program of Cox -1 | ||||||
---|---|---|---|---|---|---|
Hold | 1 Cycles | 35 Cycles | 1 Cycles | Cycles | ||
Final Extention | Extention | Anneling | Denaturation | Initial Denaturation | STEP | |
4 C | 72 C | 72 C | 52 C | 95 C | 95 C | TEMP |
∞ | 7 min | 45 sec | 1 min | 30 sec | 3 min | TIME |
Cox-1 | Cox1-F | 5′-TGGTGGTTTAAGTGTTTGACTG-3′ | |
Cox1-R | 5′-CCAACAACAAAGGCAACATT-3′ | ||
ITS1-5.8s-ITS2 rDNA region | Physa-F | 5′-GCGAAC GGC TCA TTA TAA CA-3′ | |
Physa-R | 5′-AAT TTCACC TCT CAC GCA-3′ |
PCR program of ITS1-5.8s-ITS2 rDNA | ||||||
---|---|---|---|---|---|---|
Hold | 1 Cycles | 35 Cycles | 1 Cycles | Cycles | ||
Final Extention | Extention | Anneling | Denaturation | Initial Denaturation | STEP | |
4 C | 72 C | 72 C | 54 C | 95 C | 95 C | TEMP |
∞ | 7 min | 1 min | 1 min | 30 sec | 3 min | TIME |