Uneingeschränkter Zugang

Occurrence and phylogenetic description of cystic echinococcosis isolate from Egyptian camel (Camelus dromedarius)

I. S. Elshahawy
I. S. Elshahawy
, 
M. A. El-Seify
M. A. El-Seify
, 
Z. K. Ahamed
Z. K. Ahamed
 und 
M. M. Fawaz
M. M. Fawaz
   | 17. Dez. 2022
Helminthologia's Cover Image
Über diesen Artikel
Vorheriger Artikel
Nächster Artikel
Zitieren
Article Category: Research Article
Online veröffentlicht: 17. Dez. 2022
Seitenbereich: 253 - 264
Eingereicht: 03. Feb. 2022
Akzeptiert: 17. Aug. 2022
DOI: https://doi.org/10.2478/helm-2022-0026
Schlüsselwörter
© 2022 I. S. Elshahawy, M. A. El-Seify, Z. K. Ahamed, M. M. Fawaz, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1

a, b) Calcified cyst; c) Protoscoleces; d) Multiple fertile cysts in the lung (yellow arrow head).
a, b) Calcified cyst; c) Protoscoleces; d) Multiple fertile cysts in the lung (yellow arrow head).

Fig. 2

Agarose (1.5%) gel electrophoresis of PCR-products of cox1 gene (450bp) of E. granulosus isolates in camel samples. L: ladder, Pos.: positive control, Neg.: negative control, 1, 2, 3, 4and 5 samples (all +ve).
Agarose (1.5%) gel electrophoresis of PCR-products of cox1 gene (450bp) of E. granulosus isolates in camel samples. L: ladder, Pos.: positive control, Neg.: negative control, 1, 2, 3, 4and 5 samples (all +ve).

Fig. 3

Agarose (1.5%) gel electrophoresis of PCR-products of Nad1 gene (500bp) of E. granulosus isolates in camel samples. L: ladder, Pos.: positive control, Neg.: negative control, 1, 2, 3,4and 5 samples (all +ve).
Agarose (1.5%) gel electrophoresis of PCR-products of Nad1 gene (500bp) of E. granulosus isolates in camel samples. L: ladder, Pos.: positive control, Neg.: negative control, 1, 2, 3,4and 5 samples (all +ve).

Fig. 4

Genetic relationships of the obtained genotypes from camel in the present study and reference sequences related genotypes of E. granulosus as well as Taenia saginata as the outgroup. The relationships were inferred based on phylogenetic tree (cox1 gene). The phylogenetic tree was constructed using Maximum Likelihood Tree implemented in MEGA software version 6.
Genetic relationships of the obtained genotypes from camel in the present study and reference sequences related genotypes of E. granulosus as well as Taenia saginata as the outgroup. The relationships were inferred based on phylogenetic tree (cox1 gene). The phylogenetic tree was constructed using Maximum Likelihood Tree implemented in MEGA software version 6.

Fig. 5

Genetic relationships of the obtained genotypes from camel in the present study and reference sequences related genotypes of E. granulosus as well as Taenia saginata as the out-group. The relationships were inferred based on phylogenetic tree (nad1 gene). The phylogenetic tree was constructed using Maximum Likelihood Tree implemented in MEGA software version 6.
Genetic relationships of the obtained genotypes from camel in the present study and reference sequences related genotypes of E. granulosus as well as Taenia saginata as the out-group. The relationships were inferred based on phylogenetic tree (nad1 gene). The phylogenetic tree was constructed using Maximum Likelihood Tree implemented in MEGA software version 6.

Difference in bases at the nad1 locus of E. granulosus s.l.

Accession No. Genotype Nucleotide Substitution No. Substitution/Position Substitution/Position Substitution/Position
(MH301013) G7 (Pig, France) Three G to A 180/381/387 - -
(KX010880) G6/7 (Cattle, Ethiobia) Four G to A 180 / 381 C to T 336 A to G 432
(MT166290) G6 (Camel, Nigeria) Five G to A 180 /381/387 C to T 336 A to G 432

Comparative biochemical analysis of hydatid fluids collected from dromedary camels and cattle infected with cystic echinococcosis (mean ± SE, n = 5).

Biochemical profiles Host
Camel Cattle
Total protein (g/l) 3.76 ± 0.73 2.42 ± 0.01
Glucose (mmol/L) 3.36 ± 0.16 2.58 ± 0.47
Urea (mmol/L) 5.21 ± 0.17 4.36 ± 0.5
Cholesterol (mmol/L) 2.94 ± 0.61 2.19 ± 0.31
Creatinine (μmol/L) Calcium (mmol/L) 101.22 ± 3.09 2.937 ± 0.03 80.67 ± 0.66 3.224 ± 0.02
Triglycerides (mmol/L) 0.039 ± 0.01 0.186 ± 0.003
Sodium (mmol/L) 111.35 ± 5.45 118.09 ± 0.54
Potassium (mmol/L) 13.92 ± 7.41 10.05 ± 0.05
Magnesium (mmol/L) 1.113 ± 0.33 1.019 ± 0.02
Copper (ppm) 0.42 ± 0.11 0.338 ± 0.016

GenBank accession numbers of cox1 and nad1 of Echinococcus genotypes used in phylogenetic analysis in the current study

Accession No. for NAD1 Locality Host origin Genotype Accession No. for Cox1 Locality Host origin Genotype
MW183240 Egypt Camel G7* MW173485 Egypt Camel G7*
MW183239 Egypt Camel G7* MW173484 Egypt Camel G7*
MH301013 France Pig G7 MH301013 France Pig G7
KX010897 Hungary Pig G6/7 MH301019 Italy Pig G7
KX010890 France Pig G6/7 MH301010 France Pig G7
KX010889 Namibia Oryx G6/7 MH301009 France Pig G7
KX010886 Sudan Camel G6/7 MH301008 France Pig G7
MH301016 France Pig G7 MH300939 Sudan Camel G6
KX510135 Serbia Pig G6/7 KX010833 Kenya Camel G6/7
KX231668 Armenia Pig G6/7 MT166286 Nigeria Camel G6
KX010880 Ethiobia Cattle G6/7 MT166284 Nigeria Cattle G6
MT525964 Kenya Cattle G6/7 MH301021 Ukraine Pig G6
MT166290 Nigeria Camel G6 MH301006 Poland Pig G7
MT166289 Nigeria Cattle G6 MH301005 Poland Homosapiens G7
JN191326 Egypt Donkey G4 JN191319 Egypt Donkey G4
MK644934 South Korea Human Taenia saginata MK644934 South Korea Human Taenia saginata
eISSN:
1336-9083
Sprache:
Englisch
Zeitrahmen der Veröffentlichung:
4 Hefte pro Jahr
Fachgebiete der Zeitschrift:
Biologie, Zoologie, Ökologie, andere, Medizin, Klinische Medizin, Mikrobiologie, Virologie und Infektionsepidemiologie
Zeitschrift RSS Feed