Recently,
Nematodes were extracted using a modification of the decanting and sieving method (Flegg, 1967). For morphological studies, the nematodes were killed with hot water, fixed in a 5 % formalin solution, and mounted in glycerin on slides using the Seinhorst technique (Seinhorst, 1959). Molecular studies were performed using the scientific equipment of Core Research Facility of the “Bioengineering” Center (Moscow, Russia). For this work, nematodes frozen in distilled water were used. There were three replicates. Each replicate was a test tube with several specimens of
For this study formal consent is not required.
Light photomicrographs of
A) Entire female, B) Anterior end, C) Lateral field at mid-body, D) Posterior end
Measurements of 15 females of
Characters | Measurements |
---|---|
L | 823 ± 68 (710–930) |
Body width | 41.5 ± 4.3 (35–50) |
Pharynx length | 153 ± 12.5 (132.5–165) |
Tail length | 80 ± 9.8 (66–95) |
a | 19.8 ± 2 (17.4–24.2) |
b | 5.3 ± 0.1 (5.1–5.5) |
c | 10.5 ± 1.2 (8.9–12.5) |
c' | 2.8 ± 0.3 (2.3–3.3) |
V, % | 85.4 ± 0.8 (84.3–87.1) |
VL/VB | 3.2 ± 0.3 (2.7–3.9) |
Lip width | 19 ± 1.5 (16–22.5) |
Lip height | 6.6 ± 0.6 (5.6–7.8) |
Stylet | 85.6 ± 6 (75–92.5) |
DGO | 9.7 ± 1.2 (8–12) |
R | 236.9 ± 14.8 (206–257) |
Rst | 23.9 ± 3 (18–27) |
Roes | 42.6 ± 4.8 (36–49) |
Rex | 45.4 ± 4.5 (39–52) |
RV | 45.1 ± 2.7 (41–51) |
RVan | 13.2 ± 2.8 (10–18) |
Ran | 32.7 ± 3.1 (28–38) |
Female. Body straight to slightly ventrally arcuate. Cuticular sheath fitting closely or loosely to body, more loosely on posterior end. Lateral field with occasional breaks of transverse striae and anastomoses bordered by two lines for a short distance; sometimes it looks like two longitudinal rows of blocks. Each annulus with two rows of scratches. Labial region broad, truncated, with two distinct annuli. Stylet with posteriorly sloping knobs sized about 7 μm across. Excretory pore 3–4 annuli posterior to pharynx base. Hemizonid distinct or indistinct, two annuli long, located anterior to excretory pore. Reproductive system mono-prodelphic, outstretched, oocytes in single row except for anterior end. In studied specimens, spermatheca of only one female was filled with sperm. Eggs (n=3) 78–82 x 24–26 μm in size, one per uterus. Vulval lips modified, elongated. Tail elongate conoid, tip is usually straight; in some specimens, it is curved slightly dorsally, while on others, it is curved slightly ventrally. Males were not found.
Remarks. Morphology and morphometric data of the Moscow population of
Molecular characterization. The sequences of the 18S rRNA gene, the D2-D3 expansion segments of the 28S rRNA gene, and the ITS1 obtained from different individuals in this study were identical within each marker. The sequences of the 18S rRNA gene of the studied specimens were identical to the sequence AJ966471.
Three other sequences of
The sequences of the D2–D3 expansion segments of the 28S rRNA gene were identical to the sequences of
The sequences of Internal transcribed spacer 1 (ITS1) were identical to the sequence of