<abstract xmlns="http://www.w3.org/1999/xhtml"><p>A potential protective effect of probiotic strains against zoonotic <italic>Trichinella spiralis</italic> infection was investigated in the framework of a new therapeutic strategy aimed at using probiotics to control parasitic zoonoses. The study was focused on the impact of six selected probiotic (bacteriocinogenic) strains on the intensity of <italic>T</italic>. <italic>spiralis</italic> infection and female fecundity <italic>ex vivo</italic> and <italic>in vitro</italic>. Bacterial strains of different origin (<italic>Enterococcus faecium</italic> EF55, <italic>Enterococcus faecium</italic> 2019 = CCM7420, <italic>Enterococcus faecium</italic> AL41 = CCM8558, <italic>Enterococcus durans</italic> ED26E/7, <italic>Lactobacillus fermentum</italic> AD1 = CCM7421, <italic>Lactobacillus plantarum</italic> 17L/1) were administered daily in a dose of 10<sup>9</sup> CFU/ml in 100 μl, and mice were infected with 400 <italic>T</italic>. <italic>spiralis</italic> larvae on day 7 of treatment. Female adults of <italic>T</italic>. <italic>spiralis</italic> were isolated on day 5 post infection (p.i.) and subsequently were used in fecundity test <italic>ex vivo</italic>. <italic>E</italic>. <italic>faecium</italic> CCM8558, <italic>E</italic>. <italic>faecium</italic> CCM7420 and <italic>E</italic>. <italic>durans</italic> ED26E/7 strains significantly reduced the number of adults in the intestine. The application of <italic>L</italic>. <italic>fermentum</italic> CCM7421, <italic>L</italic>. <italic>plantarum</italic> 17L/1, <italic>E</italic>. <italic>faecium</italic> CCM8558 and <italic>E</italic>. <italic>durans</italic> ED26E/7 caused a significant decrease in the number of muscle larvae. The treatment with <italic>E</italic>. <italic>faecium</italic> CCM8558 and <italic>E</italic>. <italic>durans</italic> ED26E/7 showed the highest inhibitory effect on female fecundity (94 %). The number of newborn larvae (NBL) was also significantly decreased after administration of <italic>L</italic>. <italic>fermentum</italic> CCM7421 and <italic>L</italic>. <italic>plantarum</italic> 17L/1 (80 %). A direct impact of probiotic strains on female reproductive capacity was examined <italic>in vitro</italic> in females isolated from untreated infected mice on day 5 p.i. A correlation was found between the inhibitory effect and the concentration of probiotic strains. The reduction effects of the strains manifested as follows: <italic>L</italic>. <italic>fermentum</italic> CCM7421 (93 %), <italic>E</italic>. <italic>faecium</italic> CCM8558, <italic>L</italic>. <italic>plantarum</italic> 17L/1, <italic>E</italic>. <italic>faecium</italic> EF55 (about 80 %), <italic>E</italic>. <italic>faecium</italic> CCM7420 and <italic>E</italic>. <italic>durans</italic> ED26E/7 (about 60 %).</p></abstract>

A potential protective effect of probiotic strains against zoonotic Trichinella spiralis infection was investigated in the framework of a new therapeutic strategy aimed at using probiotics to control parasitic zoonoses. The study was focused on the impact of six selected probiotic (bacteriocinogenic) strains on the intensity of T. spiralis infection and female fecundity ex vivo and in vitro. Bacterial strains of different origin (Enterococcus faecium EF55, Enterococcus faecium 2019 = CCM7420, Enterococcus faecium AL41 = CCM8558, Enterococcus durans ED26E/7, Lactobacillus fermentum AD1 = CCM7421, Lactobacillus plantarum 17L/1) were administered daily in a dose of 109 CFU/ml in 100 μl, and mice were infected with 400 T. spiralis larvae on day 7 of treatment. Female adults of T. spiralis were isolated on day 5 post infection (p.i.) and subsequently were used in fecundity test ex vivo. E. faecium CCM8558, E. faecium CCM7420 and E. durans ED26E/7 strains significantly reduced the number of adults in the intestine. The application of L. fermentum CCM7421, L. plantarum 17L/1, E. faecium CCM8558 and E. durans ED26E/7 caused a significant decrease in the number of muscle larvae. The treatment with E. faecium CCM8558 and E. durans ED26E/7 showed the highest inhibitory effect on female fecundity (94 %). The number of newborn larvae (NBL) was also significantly decreased after administration of L. fermentum CCM7421 and L. plantarum 17L/1 (80 %). A direct impact of probiotic strains on female reproductive capacity was examined in vitro in females isolated from untreated infected mice on day 5 p.i. A correlation was found between the inhibitory effect and the concentration of probiotic strains. The reduction effects of the strains manifested as follows: L. fermentum CCM7421 (93 %), E. faecium CCM8558, L. plantarum 17L/1, E. faecium EF55 (about 80 %), E. faecium CCM7420 and E. durans ED26E/7 (about 60 %).

The anti-parasitic effect of probiotic bacteria <italic>via</italic> limiting the fecundity of <italic>Trichinella spiralis</italic> female adults

Institute of Parasitology, Slovak Academy of Sciences

Institute of Animal Physiology – Centre of Biosciences, Slovak Academy of Sciences

University of Veterinary Medicine and Pharmacy in Košice

Helminthologia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The anti-parasitic effect of probiotic bacteria  limiting the fecundity of  female adults

A potential protective effect of probiotic strains against zoonotic Trichinella spiralis infection was investigated in the framework of a new therapeutic...

The anti-parasitic effect of probiotic bacteria via limiting the fecundity of Trichinella spiralis female adults

Online veröffentlicht: 26. Apr. 2018

Seitenbereich: 102 - 111

Eingereicht: 11. Jan. 2018

Akzeptiert: 14. März 2018

DOI: https://doi.org/10.2478/helm-2018-0010

Schlüsselwörter
female fecundity, probiotic bacteria,

© 2018 B. Bucková et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The anti-parasitic effect of probiotic bacteria via limiting the fecundity of Trichinella spiralis female adults

Online veröffentlicht: 26. Apr. 2018

Seitenbereich: 102 - 111

Eingereicht: 11. Jan. 2018

Akzeptiert: 14. März 2018

DOI: https://doi.org/10.2478/helm-2018-0010

Schlüsselwörterfemale fecundity, probiotic bacteria,

© 2018 B. Bucková et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Schlüsselwörter
female fecundity, probiotic bacteria,