<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>About 150 million families around the world are engaged in milk production. However, inflammation of the mammary gland (mastitis) remains a major problem in dairy ruminants that affects the quality of milk worldwide. The aim of this study was the examination of udder health with detection of contagious and environmental pathogens causing mastitis in 960 and 940 dairy cows and ewes, respectively. The presence of selected virulence factors such as: the formation of haemolysis, gelatinase, biofilm, hydrolyse DNA, and resistance to antibiotics with detection of methicillin resistance gene (<italic>mecA</italic>), were determined in selected virulence factors associated with isolated staphylococci. These isolated staphylococci with selected virulence factors can have untoward effects on the severity of mastitis. The results of our study indicated that, in addition to the major udder pathogens (<italic>S. aureus, S. uberis,</italic> and <italic>S. agalactiae</italic>) causing mastitis, non-aureus staphylococci (NAS), is a major risk to dairy cows and ewes. NAS, such as <italic>S. chromogenes</italic>, <italic>S. warneri</italic>, and <italic>S. xylosus</italic> isolated from infected animals with clinical and chronic mastitis, had the highest representation of virulence factors in comparison to less virulent strains. In addition, the isolates of <italic>S. aureus</italic> and NAS demonstrated 77.0 % and 44.2 % resistance to one or more antimicrobial classes from mastitic milk samples obtained from dairy cows and ewes, respectively. Due to the high resistance to β-lactamantibiotics in two isolates of <italic>S. aureus</italic> and two species of NAS isolated from cows’ mastitic milk samples, the presence of a methicillin-resistant gene <italic>mecA</italic> poses serious complications for the treatment and a serious health risk to milk consumers.</p>
</abstract>

About 150 million families around the world are engaged in milk production. However, inflammation of the mammary gland (mastitis) remains a major problem in dairy ruminants that affects the quality of milk worldwide. The aim of this study was the examination of udder health with detection of contagious and environmental pathogens causing mastitis in 960 and 940 dairy cows and ewes, respectively. The presence of selected virulence factors such as: the formation of haemolysis, gelatinase, biofilm, hydrolyse DNA, and resistance to antibiotics with detection of methicillin resistance gene (mecA), were determined in selected virulence factors associated with isolated staphylococci. These isolated staphylococci with selected virulence factors can have untoward effects on the severity of mastitis. The results of our study indicated that, in addition to the major udder pathogens (S. aureus, S. uberis, and S. agalactiae) causing mastitis, non-aureus staphylococci (NAS), is a major risk to dairy cows and ewes. NAS, such as S. chromogenes, S. warneri, and S. xylosus isolated from infected animals with clinical and chronic mastitis, had the highest representation of virulence factors in comparison to less virulent strains. In addition, the isolates of S. aureus and NAS demonstrated 77.0 % and 44.2 % resistance to one or more antimicrobial classes from mastitic milk samples obtained from dairy cows and ewes, respectively. Due to the high resistance to β-lactamantibiotics in two isolates of S. aureus and two species of NAS isolated from cows’ mastitic milk samples, the presence of a methicillin-resistant gene mecA poses serious complications for the treatment and a serious health risk to milk consumers.

Detection of Some Virulence Factors in Staphylococci Isolated from Mastitic Cows and Ewes

Department of Nutrition and Animal Husbandry

Department of Public Veterinary Medicine and Animal Welfare University of Veterinary Medicine and Pharmacy

Department of Husbandry and Development of Animal Health Faculty of Veterinary Medicine

Department of Pathobiochemistry, Faculty of Pharmacy

Folia Veterinaria

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

About 150 million families around the world are engaged in milk production. However, inflammation of the mammary gland (mastitis) remains a major problem in...

Detection of Some Virulence Factors in Staphylococci Isolated from Mastitic Cows and Ewes

Online veröffentlicht: 30. Dez. 2022

Seitenbereich: 18 - 30

Eingereicht: 12. Aug. 2022

Akzeptiert: 07. Okt. 2022

DOI: https://doi.org/10.2478/fv-2022-0034

Schlüsselwörter
antibiotics, biofilm, dairy cows, ewes, mastitis, methicillin resistance gene, non-aureus staphylococci

© 2022 Zigo F. et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Detection of Some Virulence Factors in Staphylococci Isolated from Mastitic Cows and Ewes

Online veröffentlicht: 30. Dez. 2022

Seitenbereich: 18 - 30

Eingereicht: 12. Aug. 2022

Akzeptiert: 07. Okt. 2022

DOI: https://doi.org/10.2478/fv-2022-0034

Schlüsselwörterantibiotics, biofilm, dairy cows, ewes, mastitis, methicillin resistance gene, non-aureus staphylococci

© 2022 Zigo F. et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Schlüsselwörter
antibiotics, biofilm, dairy cows, ewes, mastitis, methicillin resistance gene, non-aureus staphylococci