<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The antifungal activities of 14 selected essential oils (at the concentrations of 0.5 %, 5 %, and 30 %) against the yeast <italic>Malassezia pachydermatis</italic> (18 isolates and one reference strain) were investigated. The isolates of <italic>M. pachydermatis</italic> were obtained from swabs of external ear canals of healthy dogs using sterile swabs. The determination of the efficacy was based on a modified disc diffusion method (CLSI M44-A2). The best antifungal efficacy (100 %) was shown by clove, cinnamon and oregano at the concentration of 30 %; less significant efficacy was shown at the concentration of 5 % (38 %, 33 % and 5 %, respectively). Satureja inhibited the growth of <italic>Malassezia</italic> (efficacy of 16 %) only at the concentration of 30 %. Bergamot, lavender, juniper, cedar, sage, tea-tree, grapefruit, pine, chamomile and yarrow essential oils were not able to form inhibition zones as defined in the methodology used (greater or equal to 15 mm) in all concentrations used. Therefore, according to the interpretation criterion, they were considered ineffective. In all cases, the concentration of 0.5 % was not effective against the growth of <italic>Malassezia</italic> yeasts.</p></abstract>

The antifungal activities of 14 selected essential oils (at the concentrations of 0.5 %, 5 %, and 30 %) against the yeast Malassezia pachydermatis (18 isolates and one reference strain) were investigated. The isolates of M. pachydermatis were obtained from swabs of external ear canals of healthy dogs using sterile swabs. The determination of the efficacy was based on a modified disc diffusion method (CLSI M44-A2). The best antifungal efficacy (100 %) was shown by clove, cinnamon and oregano at the concentration of 30 %; less significant efficacy was shown at the concentration of 5 % (38 %, 33 % and 5 %, respectively). Satureja inhibited the growth of Malassezia (efficacy of 16 %) only at the concentration of 30 %. Bergamot, lavender, juniper, cedar, sage, tea-tree, grapefruit, pine, chamomile and yarrow essential oils were not able to form inhibition zones as defined in the methodology used (greater or equal to 15 mm) in all concentrations used. Therefore, according to the interpretation criterion, they were considered ineffective. In all cases, the concentration of 0.5 % was not effective against the growth of Malassezia yeasts.

Antifungal Effect of Selected Essential Oils on <italic>Malassezia pachydermatis</italic> Growth

Institute of Pharmacology, Department of Pharmacology and Toxicology

University of Veterinary Medicine and Pharmacy

Folia Veterinaria

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Antifungal Effect of Selected Essential Oils on  Growth

The antifungal activities of 14 selected essential oils (at the concentrations of 0.5 %, 5 %, and 30 %) against the yeast Malassezia pachydermatis (18 isolates...

Antifungal Effect of Selected Essential Oils on Malassezia pachydermatis Growth

Online veröffentlicht: 17. Juli 2018

Seitenbereich: 67 - 72

Eingereicht: 27. Feb. 2018

Akzeptiert: 15. Mai 2018

DOI: https://doi.org/10.2478/fv-2018-0018

Schlüsselwörter
dog, essential oil, disc diffusion method, sensitivity

© 2018 Váczi, P. et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Antifungal Effect of Selected Essential Oils on Malassezia pachydermatis Growth

Online veröffentlicht: 17. Juli 2018

Seitenbereich: 67 - 72

Eingereicht: 27. Feb. 2018

Akzeptiert: 15. Mai 2018

DOI: https://doi.org/10.2478/fv-2018-0018

Schlüsselwörterdog, essential oil, disc diffusion method, sensitivity

© 2018 Váczi, P. et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Schlüsselwörter
dog, essential oil, disc diffusion method, sensitivity