The Expression of miR-320b in Extracellular Vesicles as a Predictor of the Progression of Non-small Cell Lung Carcinoma and Its Association with Molecular and Clinical Therapy Response

Figure 1:

Figure 2:

Figure 3:

Relationship between Treatment Response and miR-320b Levels After Therapy_

miR-320b PR group (med (min – max))	miR-320b SD group (med (min – max))	miR-320b PD group (med (min – max))	P****
0.72 (0.38 – 4.55)	0.79 (0.14 – 9.70)	1.29 (0.52 – 1.66)	0.767

miR-320b Parameter Before and After Therapy_

miR-320b Before Therapy (mean ± SD)	miR-320b After Therapy (mean ± SD)	P***
1.00 ± 0.0	1.72 ± 2.40	0.856

Characteristic of Study Subjects_

Clinical Characteristics of Study Subjects (n = 16)	Mean ± SD	Frequency n (%)
Age (years)	53.44 ± 10.92
Gender
Male		11 (68.8)
Female		5 (31.3)
Weight (kg)	57.66 ± 10.54
Height (m)	1.63 ± 0.09
BMI	21.72 ± 3.70
Duration (months)	9.44 ± 2.63
Treatment option
Chemotherapy		13 (81.3)
Tyrosine kinase inhibitor		3 (18.8)
COVID-19 history
Present		3 (18.8)
None		13 (81.2)
Cancer history
None		16 (100)
Stage
Stage IV		16 (100)
Comorbidities
Hypertension		1 (6.3)
Diabetes mellitus		2 (12.5)
Cardiac disease (CAD)		1 (6.3)
Cataract		1 (6.3)
None		11 (68.8)

Symptoms and Laboratory Parameters of Research Subjects_

Parameters	Before therapy n (%)	After therapy n (%)	Before therapy (mean± SD)	After therapy (mean± SD)	P
Symptoms
Cough	3 (18.8)	1 (6.3)			0.625*
Hemoptysis	4 (25.0)	0 (0)			0.125*
Shortness of breath	10 (62.5)	1 (6.3)			0.004*
Chest pain	2 (12.5)	0 (0)			0.500*
Metastasis symptoms	6 (37.5)	2 (12.5)			0.125*
Laboratory
Hemoglobin			12.38 ± 2.02	11.52 ± 1.94	0.122**
Leucoytes			10.90 ± 5.82	8.52 ± 4.88	0.034**
Platelets			377.56 ± 151.36	359.50 ± 154.30	0.650***

Relationship between Treatment Type and miR-320b Levels_

Treatment	miR-320b (med (min – max))	P*****
Chemotherapy	0.93 (0.14 – 9.70)	0.737
TKI	0.45 (0.38 – 4.55)

Relationship between miR-320b and Mortality_

Mortality	miR-320b (med (min – max))	P*****
Yes	0,93 (0,14 – 2,76)	0,791
No	0,85 (0,34 – 9,7)

Sensitivity and Specificity of miR-320b for Predicting Treatment Response_

Positive for CR/PR if miR-320B is greater than or equal to	Sensitivity	1 - Specificity
0.240	1.000	0.900
0.360	1.000	0.800
0.400	0.833	0.800
0.435	0.667	0.800
0.485	0.500	0.800
0.620	0.500	0.700
0.785	0.500	0.600
0.890	0.500	0.500
0.960	0.500	0.400
1.195	0.333	0.400
1.525	0.167	0.400
1.655	0.167	0.300
2.210	0.167	0.200
3.655	0.167	0.100