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IgA and IgG antibodies in SARS-CoV-2 vaccinated health workers by a homemade ELISA diagnostic test

Tagliaferro Luigi
Tagliaferro Luigi
, 
Menegazzi Paola
Menegazzi Paola
, 
Cossa Luca Giulio
Cossa Luca Giulio
 und 
Maffia Michele
Maffia Michele
   | 17. Juni 2021
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Article Category: COVID-19 Special Issue
Online veröffentlicht: 17. Juni 2021
Seitenbereich: 13 - 19
DOI: https://doi.org/10.2478/ebtj-2021-0016
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© 2021 Tagliaferro Luigi, Menegazzi Paola, Cossa Luca Giulio, Maffia Michele, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1

Histograms show the characterization of serum of health workers: (A) anti-RBD ectodomain Spike protein IgG; (B) anti-nucleocapside protein IgG; (C) anti-RBD ectodomain Spike protein IgA; (D) anti-nucleocapside protein IgA.
Histograms show the characterization of serum of health workers: (A) anti-RBD ectodomain Spike protein IgG; (B) anti-nucleocapside protein IgG; (C) anti-RBD ectodomain Spike protein IgA; (D) anti-nucleocapside protein IgA.

Figure 2

Box & Whiskers plots show the serological level of IgG and IgA anti-RBD ectodomain Spike protein (A) and anti-nucleocapsid protein (B) of SARS-CoV-2. Tukey's multiple comparisons test was used (** P value < 0.005).
Box & Whiskers plots show the serological level of IgG and IgA anti-RBD ectodomain Spike protein (A) and anti-nucleocapsid protein (B) of SARS-CoV-2. Tukey's multiple comparisons test was used (** P value < 0.005).

IgG and IgA levels in the serum of each health worker who underwent serological test.

Anti-S (RBD) antibody Anti-N antibody
IgG Cut off: 0.277 OD IgA Cut off: 0.290 OD IgG Cut off: 0.326 OD IgA Cut off: 0.381 OD
OD index/cutoff Result OD index/cutoff Result OD index/cutoff Result OD index/cutoff Result
Vaccine Dose Age 0.90-1.10 0.90-1.10 0.90-1.10 0.90-1.10
LT ♂ 0 60 0.08 - 0.00 - 0.29 - 0.03 -
LT I 0.93 +/- 0.70 - 0.38 - 0.03 -
LT II 2.63 + 1.09 +/- 0.46 - 0.11 -
ARB ♀ I 61 3.16 + 0.10 - 0.42 - 0.00 -
ARB II 2.45 + 2.49 + 1.23 + 0.09 -
VD ♀ I 38 0.87 - 0.42 - 0.44 - 0.74 -
VD II 3.13 + 1.17 + 0.37 - 0.62 -
IDP ♀ I 40 1.92 + 0.23 - 0.59 - 0.14 -
IDP II 2.53 + 0.88 - 0.57 - 0.09 -
PM ♀ I 52 1.71 + 0.09 - 0.21 - 0.07 -
PM II 3.11 + 0.31 - 0.22 - 0.06 -
AM ♂ 0 59 0.30 - 0.05 - 1.36 + 0.04 -
AM I 2.84 + 0.19 - 1.04 +/- 0.18 -
AM II 2.71 + 0.09 - 0.79 - 0.02 -
AC ♀ II 58 2.77 + 0.48 - 0.10 - 0.02 -
ACP ♀ II 28 2.29 + 0.41 - 0.40 - 0.03 -
MR ♀ I 56 3.01 + 1.70 + 0.17 - 0.08 -
MR II 2.27 + 2.30 + 0.13 - 0.16 -
MT ♀ I 56 0.26 - 0.23 - 0.67 - 0.09 -
MT II 3.49 + 0.36 - 0.87 - 0.07 -
MLV ♀ I 38 3.48 + 1.94 + 0.33 - 0.13 -
MLV II 2.51 + 1.13 + 0.08 - 0.01 -
RR ♂ I 35 1.09 +/- 0.10 - 0.09 - 0.00 -
RR II 2.77 + 0.63 - 0.15 - 0.04 -
GDA ♂ I 69 0.32 - 0.00 - 0.26 - 0.03 -
GDA II 2.88 + 0.61 - 0.23 - 0.02 -
BR ♂ I 42 0.83 - 0.09 - 0.23 - 0.06 -
BR II 2.53 + 0.72 - 0.31 - 0.05 -
CP ♀ I 53 2.53 + 0.29 - 0.49 - 0.13 -
CP II 2.87 + 0.28 - 0.11 - 0.04 -
MG ♀ I 47 0.41 - 0.00 - 0.17 - -0.01 -
MG II 3.22 + 0.37 - 0.14 - 0.04 -
PT ♂ I 59 0.95 +/- 0.06 - 1.73 + 0.04 -
PT II 2.80 + 0.28 - 1.53 + 0.03 -
LM ♀ I 57 0.79 - 0.02 - 0.27 - 0.04 -
LM II 2.99 + 0.53 - 0.30 - 0.03 -
DS ♀ I 43 2.26 + 0.20 - 1.37 + 0.15 -
DS II 2.68 + 0.02 - 0.78 - 0.02 -
MF ♀ I 51 0.16 - -0.04 - 0.10 - -0.04 -
MF II 2.70 + 0.05 - 0.13 - 0.01 -
BS ♀ II 31 2.87 + 0.12 - 0.36 - 0.05 -
SE ♀ II 3.03 + 0.02 - 0.38 - 0.02 -
RA ♀ II 59 3.96 + 0.27 - 0.32 - 0.02 -
GAM ♀ II 65 3.04 + 0.17 - 0.10 - 0.06 -

Characterization of the health workers' serum.

Results Anti-S Anti-N
IgG IgA IgG IgA
1st dose Negative 7 16 15 18
Borderline 3 0 1 0
Positive 8 2 2 0
total 18 18 18 18
2nd dose Negative 0 19 22 24
Borderline 0 1 0 0
Positive 24 4 2 0
total 24 24 24 24
Zero time Negative 2 2 1 2
Borderline 0 0 0 0
Positive 0 0 1 0
total 2 2 2 2
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