Fungal elimination of 2,4,6-trinitrotoluene (TNT) from the soils
, , , , , und | 06. Feb. 2018
Über diesen Artikel
Article Category: Research Article
Online veröffentlicht: 06. Feb. 2018
Seitenbereich: 39 - 46
DOI: https://doi.org/10.2478/ebtj-2018-0007
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© 2018 Lali Kutateladze, Nino Zakariashvili, Izolda Khokhashvili, Maya Jobava, Tinatin Alexidze, Tamar Urushadze, Edisher Kvesitadze, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.
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Main products of biotransformation of (1-14C)-Trinitrotoluene in culture liquid
| Strain | Radioactivity, in% | |
|---|---|---|
| Organic acids | Amino acids | |
| 72.2 | 27.8 | |
| 77.4 | 22.6 | |
| 89.8 | 10.2 | |
Changes of CFU of introduced and indigenous fungi in TNT contaminated soils under natural modeling conditions
| Test variant | CFU of fungi per 1g of soil | ||
|---|---|---|---|
| At the moment of inoculation | 40 days later | 100 days later | |
| Red soil (sterile soil) + TNT | 0 | 0 | 0 |
| Red soil (nonsterile soil) + TNT | 3.8 | 1.2 | 1.3 |
| Red soil (nonterile soil) + TNT + | 5.4 | 3.9 | 4.9 |
| Red soil (nonsterile soil) + TNT + | 1.9 | 7.2 | 1.3 |
| Black soil (sterile soil) + TNT | 0 | 0 | 0 |
| Black soil (nonsterile soil) + TNT | 6.5 | 4.7 | 3.2 |
| Black soil (nonsterile soil) + TNT + | 5 | 8.8 | 5.7 |
| Black soil (nonsterile soil) + TNT + | 1.9 | 7.0 | 2.4 |
The amount of (1-14C)-TNT detected in fungi biomass, in%
| Culture | Nutrient medium #2 | Biomass, mg | Total radioactivity of biomass, in % |
|---|---|---|---|
| Czapek’s medium | 29 | 53.6 | |
| Czapek’s medium | 25 | 51.9 | |
| Czapek’s medium | 27 | 52.9 |
Changes of CFU of fungi introduced into TNT-contaminated soils
| Strain | Soil types | CFU per g of soil | |||
|---|---|---|---|---|---|
| Initial amount | 10 days later | 20 days later | 30 days later | ||
| Black soil | 7,5x105 | 9,5x106 | 6,5x105 | 3,5x104 | |
| Red soil | 7,5x105 | 5,5x105 | 3,5x104 | 2,3x104 | |
| Black soil | 8,5x106 | 4,5x107 | 5,5x105 | 6,5x104 | |
| Red soil | 8,5.106 | 4,5x106 | 3,5x105 | 4,5x104 | |
Radioactivity of amino acids products of (1-14С) TNT transformation by fungi strains
| Strain | Radioactivity of amino acids in % | Distribution of radioactivity in individual amino acids, in % | |||||
|---|---|---|---|---|---|---|---|
| Phenylalanine | Glutamic acid | Tyrosine | Arginine | Asparaginic acid | Serine | ||
| 27.8 | 27.4 | 19.3 | 17.5 | 13.6 | 11.5 | 10.7 | |
| 22.6 | 24.3 | 16.6 | 18.7 | 18.5 | 11.5 | 10.4 | |
| 10.2 | 38.9 | 14.7 | 12.8 | 11.4 | 11.8 | 10.4 | |
Distribution of radioactivity of (1-14С)-trinitrotoluene transformation by fungi strains among organic acids
| Strain | Total radioactivity in %, of low molecular compounds fraction | Distribution of radioactivity in organic acids, in % | |||||
|---|---|---|---|---|---|---|---|
| Fumaric acid | Succinic acid | Glycolic acid | Citric acid | Malic acid | Unknown | ||
| 72.0 | 86.6 | 4.1 | 2.8 | 2.7 | 1.8 | 2.0 | |
| 77.4 | 91.8 | 4.0 | 1.5 | 1.1 | 0.8 | 0.8 | |
| 89.8 | 88.8 | 3.3 | 3.7 | 1.2 | 0.7 | 2.3 | |