<abstract xmlns="http://www.w3.org/1999/xhtml"><p>A procedure has been developed for a simple and specific determination of catechol in cigarette smoke condensate. The analysis is performed by high-performance liquid chromatography (HPLC) with column switching between a precolumn and an analytical column. Catechol is separated from most of the condensate components by selective adsorption on a dihydroxyboryl silica gel precolumn. It is then determined without interference on a reverse-phase (C<sub>18</sub>) column. By column switching this procedure is run automatically within 15 min. The method is well suited for the routine measurement of a large number of samples, since it allows condensate solutions to be injected directly into the analysis system without prior laborious and time-consuming clean-up procedures.</p></abstract>

A procedure has been developed for a simple and specific determination of catechol in cigarette smoke condensate. The analysis is performed by high-performance liquid chromatography (HPLC) with column switching between a precolumn and an analytical column. Catechol is separated from most of the condensate components by selective adsorption on a dihydroxyboryl silica gel precolumn. It is then determined without interference on a reverse-phase (C18) column. By column switching this procedure is run automatically within 15 min. The method is well suited for the routine measurement of a large number of samples, since it allows condensate solutions to be injected directly into the analysis system without prior laborious and time-consuming clean-up procedures.

Determination of Catechol in Cigarette Smoke Condensate by High-Performance Liquid Chromatography (HPLC) Analysis with an Automated Precolumn Sample Preparation

INBIFO Institut für biologische Forschung GmbH, Köln, Germany

Contributions to Tobacco & Nicotine Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

A procedure has been developed for a simple and specific determination of catechol in cigarette smoke condensate. The analysis is performed by high-performance...

Determination of Catechol in Cigarette Smoke Condensate by High-Performance Liquid Chromatography (HPLC) Analysis with an Automated Precolumn Sample Preparation

Online veröffentlicht: 06. Jan. 2015

Seitenbereich: 239 - 242

Eingereicht: 18. Dez. 1985

Akzeptiert: 24. Apr. 1986

DOI: https://doi.org/10.2478/cttr-2013-0573

© 1986 Beiträge zur Tabakforschung International

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.