<abstract xmlns="http://www.w3.org/1999/xhtml">
				
				<p> Ten successive 1 kg samples of cigarette smoke condensate were prepared and the neutrals were removed by solvent partitioning of each kg. After removal of a control sample (41.2 %), the remainder of the neutrals were separated by adsorption chromatography on silicic acid followed by partitioning of the eluates between polar and nonpolar solvents, yielding a total of 10 fractions for biological study. A range of recoveries from 94-109 % of the neutrals was obtained in the ten successive runs; the overall average recovery was 102 %. Small amounts of strongly adsorbed material not eluted from the adsorption columns by the technic could be removed in part by other procedures. Using this method, benzo[a]pyrene was concentrated to a high degree in one fraction, thus permitting the detection of biologically active nonpolynuclear agents in the other fractions on bioassay. Exclusion of light during separation did not alter significantly the benzo[a]pyrene levels obtained in the polynuclear-enriched fraction. </p>
			</abstract>

Ten successive 1 kg samples of cigarette smoke condensate were prepared and the neutrals were removed by solvent partitioning of each kg. After removal of a control sample (41.2 %), the remainder of the neutrals were separated by adsorption chromatography on silicic acid followed by partitioning of the eluates between polar and nonpolar solvents, yielding a total of 10 fractions for biological study. A range of recoveries from 94-109 % of the neutrals was obtained in the ten successive runs; the overall average recovery was 102 %. Small amounts of strongly adsorbed material not eluted from the adsorption columns by the technic could be removed in part by other procedures. Using this method, benzo[a]pyrene was concentrated to a high degree in one fraction, thus permitting the detection of biologically active nonpolynuclear agents in the other fractions on bioassay. Exclusion of light during separation did not alter significantly the benzo[a]pyrene levels obtained in the polynuclear-enriched fraction.

Composition Studies on Tobacco XL: Large Scale Fractionation of the Neutrals of Cigarette Smoke Condensate Using Adsorption Chromatography and Solvent Partitioning

Eastern Utilization Research and Development Division, Agricultural Research Service, United States Department of Agriculture, Philadelphia, Pennsylvania, USA,

Roswell Park Memorial Institute (New York State Department of Health), Buffalo, New York, USA

Contributions to Tobacco & Nicotine Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Ten successive 1 kg samples of cigarette smoke condensate were prepared and the neutrals were removed by solvent partitioning of each kg. After removal of a...

Composition Studies on Tobacco XL: Large Scale Fractionation of the Neutrals of Cigarette Smoke Condensate Using Adsorption Chromatography and Solvent Partitioning

Online veröffentlicht: 12. Juli 2014

Seitenbereich: 109 - 114

Eingereicht: 24. Nov. 1969

DOI: https://doi.org/10.2478/cttr-2013-0225

© Beiträge zur Tabakforschung International

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.