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Determination of β-blocking receptor drugs in silica gel TLC systems with the mobile phase containing surfactant


Eight drugs blocking beta-adrenergic receptors activity (acebutolol, alprenolol, atenolol, oxprenolol, labetalol, metoprolol, propranolol and sotalol) were investigated through the use of the thin-layer technique with its mobile phase containing surfactant. Assessment of the effect of surfactant presence and 1-propanol concentration in the mobile phase on the retention and separation of investigated solutes was then carried out wherein the effect of the surfactant concentration on the zone shape properties (asymmetry and tailing coefficient) was investigated. The method was applied for the quantitative analysis of the chosen solutes, and the LOD and LOQ values of chosen were determined. These were as follows: acebutolol – 1.11 and 3.36 μg/spot, metoprolol 1.45 μg/spot, 4.4 μg/spot. The chosen system is environmentally friendly due to using silica gel plates and only 5% of propanol in water.

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Medizin, Klinische Medizin, andere, Pharmakologie, Toxikologie, Pharmazie