The study of phenolic compounds and antioxidant activity of raw materials of Heliopsis helianthoides (l.) sweet

Using high performance liquid chromatography (HPLC), we studied the chemical composition and antioxidant activity of bioactive substances in the roots, leaves, flowers and seeds of Heliopsis helianthoides. The results of our study showed the presence of 5 phenolic compounds in its roots, 4 phenolic compounds in its leaves, 10 phenolic compounds in its flowers and 8 phenolic compounds in its seeds. The highest content of identified compounds was found in the leaves of this plant – 3192.20±79.78 mg/kg. The dominating hydroxycinnamic acid was chlorogenic acid. This had its highest concentration (1537.21±38.43 mg/kg) in the Heliopsis helianthoides leaves. Among flavonoids, luteolin prevailed in the roots, apigenin-7-glucoside prevailed in the seeds and rutin prevailed in the leaves and flowers. Maximum rutin content (1426.64±35.67 mg/kg) was found in the Heliopsis helianthoides leaves.

Antioxidant activity study in vitro uncovered the substantial antioxidant potential of bioactive substances (BASs) in all tested samples of the raw materials, being within the limits of 2.81-8.13 mg/g. Most active in this respect were Heliopsis helianthoides leaves. The obtained data indicate the feasibility of the development of new antioxidant active drugs on the basis of raw materials of Heliopsis helianthoides.