<abstract xmlns="http://www.w3.org/1999/xhtml"> Cimicifuga racemosa, is a plant with a diverse and long history of medicinal use. Caffeic acid, bioactive compound, which often occurs with other polyphenols can influence the biological activity of this plant. The aim of our work was quantitative analysis of caffeic acid in roots and rhizomes of two varieties of C. racemosa. Analysis was performed by HPLC method. The extracts were separated on C18 reversed-phase column using mixture of methanol, water and formic acid (25:75:0.5 v/v/v) as a mobile phase. The flow rate of eluent was 1.0 ml·min-1. The obtained validation parameters such as linearity, linear regression equation and precision expressed as a relative standard deviation were adequate for quantitative determination. Caffeic acid was found in all tested extracts. The highest total amount of caffeic acid was determined in C. racemosa var. racemosa (255.3 μg·g-1) while its concentration in C. racemosa var. cordifolia was significantly lower (213.0 μg·g-1).</abstract>

Cimicifuga racemosa, is a plant with a diverse and long history of medicinal use. Caffeic acid, bioactive compound, which often occurs with other polyphenols can influence the biological activity of this plant. The aim of our work was quantitative analysis of caffeic acid in roots and rhizomes of two varieties of C. racemosa. Analysis was performed by HPLC method. The extracts were separated on C18 reversed-phase column using mixture of methanol, water and formic acid (25:75:0.5 v/v/v) as a mobile phase. The flow rate of eluent was 1.0 ml·min-1. The obtained validation parameters such as linearity, linear regression equation and precision expressed as a relative standard deviation were adequate for quantitative determination. Caffeic acid was found in all tested extracts. The highest total amount of caffeic acid was determined in C. racemosa var. racemosa (255.3 μg·g-1) while its concentration in C. racemosa var. cordifolia was significantly lower (213.0 μg·g-1).

Determination of caffeic acid in root and rhizome of Black cohosh (Cimicifuga racemosa (L.) Nutt.)

Department of Analytical Chemistry, Medical University of Lublin, Chodzki 4a, 20-093, Lublin, Poland

Botanical Garden of Maria Curie-Skłodowska University in Lublin, Slawinkowska 3, 20-810 Lublin, Poland

Current Issues in Pharmacy and Medical Sciences

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Cimicifuga racemosa, is a plant with a diverse and long history of medicinal use. Caffeic acid, bioactive compound, which often occurs with other polyphenols...

Determination of caffeic acid in root and rhizome of Black cohosh (Cimicifuga racemosa (L.) Nutt.)

Online veröffentlicht: 25. Nov. 2014

Seitenbereich: 108 - 110

Eingereicht: 07. Juli 2014

Akzeptiert: 17. Juli 2014

DOI: https://doi.org/10.2478/cipms-2014-0025

Schlüsselwörter
caffeic acid, Cimicifuga racemosa, phenolic acids

© 2014

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Determination of caffeic acid in root and rhizome of Black cohosh (Cimicifuga racemosa (L.) Nutt.)

Online veröffentlicht: 25. Nov. 2014

Seitenbereich: 108 - 110

Eingereicht: 07. Juli 2014

Akzeptiert: 17. Juli 2014

DOI: https://doi.org/10.2478/cipms-2014-0025

Schlüsselwörtercaffeic acid, Cimicifuga racemosa, phenolic acids

© 2014

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Schlüsselwörter
caffeic acid, Cimicifuga racemosa, phenolic acids