<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Spondyloarthropathies (SpAs), are a group of chronic inflammatory diseases with a number of genetic, physiopathological, clinical and radiological features. Ankylosing spondylitis (AS) is the most common type of spondylo-arthropathies, and >90.0% of patients with ankylosing spondylitis are human leukocyte antigen-B27 <italic>(HLA-B2</italic> 7)-positive. In recent years, <italic>non-HLA</italic> genetic factors have been reported to have an effect on ankylosing spondylitis. MicroRNAs (miRNAs), are endogenous non coding RNA molecules containing 18-23 nucleotides that play a role in the post-transcriptional regulation of gene expression. In this study, we aimed to determine the expression levels of miRNAs associated with T- and B-cell differentiation/stimulation in peripheral blood mononuclear cells and their relationship with the etiology of the AS in patients and healthy controls. In a molecular study, peripheral blood mononuclear cell isolation, and total RNA isolation were performed first. In the second step, cDNA synthesis and quantitative real-time PCR (qPCR) expression analysis were completed. Ultimately, in the patient and control group, the expression levels of miR-142-5p and miR-143 were found to be significantly different (<italic>p</italic> &lt;0.05). According to current knowledge, miR-142-5p andmiR-143 expressions were found to be important for those diseases that share similar etiology with AS. We suggest that miR-142-5p and miR-143 may play a role in the pathogenesis, especially miR- 142-5p may be a potential biomarker and a target molecule for the treatment.</p></abstract>

Spondyloarthropathies (SpAs), are a group of chronic inflammatory diseases with a number of genetic, physiopathological, clinical and radiological features. Ankylosing spondylitis (AS) is the most common type of spondylo-arthropathies, and >90.0% of patients with ankylosing spondylitis are human leukocyte antigen-B27 (HLA-B2 7)-positive. In recent years, non-HLA genetic factors have been reported to have an effect on ankylosing spondylitis. MicroRNAs (miRNAs), are endogenous non coding RNA molecules containing 18-23 nucleotides that play a role in the post-transcriptional regulation of gene expression. In this study, we aimed to determine the expression levels of miRNAs associated with T- and B-cell differentiation/stimulation in peripheral blood mononuclear cells and their relationship with the etiology of the AS in patients and healthy controls. In a molecular study, peripheral blood mononuclear cell isolation, and total RNA isolation were performed first. In the second step, cDNA synthesis and quantitative real-time PCR (qPCR) expression analysis were completed. Ultimately, in the patient and control group, the expression levels of miR-142-5p and miR-143 were found to be significantly different (p <0.05). According to current knowledge, miR-142-5p andmiR-143 expressions were found to be important for those diseases that share similar etiology with AS. We suggest that miR-142-5p and miR-143 may play a role in the pathogenesis, especially miR- 142-5p may be a potential biomarker and a target molecule for the treatment.

The expression levels of microRNAs associated with T and B cell differentiation/stimulation in ankylosing spondylitis

Balkan Journal of Medical Genetics

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Spondyloarthropathies (SpAs), are a group of chronic inflammatory diseases with a number of genetic, physiopathological, clinical and radiological features....

The expression levels of microRNAs associated with T and B cell differentiation/stimulation in ankylosing spondylitis

Article Category: Original Article

Online veröffentlicht: 26. Aug. 2020

Seitenbereich: 25 - 32

DOI: https://doi.org/10.2478/bjmg-2020-0006

Schlüsselwörter
Ankylosing spondylitis (AS), Expression level of microRNA (miRNA), miR-142-5p, miR-143

© 2020 Türkyilmaz A, Ata P, Akbaş F, Yağci İ, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The expression levels of microRNAs associated with T and B cell differentiation/stimulation in ankylosing spondylitis

Article Category: Original Article

Online veröffentlicht: 26. Aug. 2020

Seitenbereich: 25 - 32

DOI: https://doi.org/10.2478/bjmg-2020-0006

SchlüsselwörterAnkylosing spondylitis (AS), Expression level of microRNA (miRNA), miR-142-5p, miR-143

© 2020 Türkyilmaz A, Ata P, Akbaş F, Yağci İ, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Schlüsselwörter
Ankylosing spondylitis (AS), Expression level of microRNA (miRNA), miR-142-5p, miR-143