<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Antimicrobial resistance properties of 32 <italic>Aeromonas</italic> strains isolated from fresh scallops (<italic>Patinopecten yessoensis</italic> (Jay)) marketed in Korea were assessed. All the <italic>Aeromonas</italic> spp., including <italic>A. salmonicida</italic>, were mesophilic and grew very well at 37°C. The isolates were tested for susceptibility to 19 antimicrobials belonging to eight antimicrobial classes. All isolates were multi-drug resistant, which means they were resistant to five or more antimicrobials. Higher resistance rates (≥ 50%) were observed for ampicillin, piperacillin, cephalothin, imipenem, meropenem, trimethoprim-sulfamethoxazole, tetracycline, oxytetracycline, and nalidixic acid while intermediate resistance was also determined. PCR assays revealed the presence of many antimicrobial resistance genes among the isolates in varying combinations. Among them, some isolates harbored higher numbers of resistant genes, e.g., <italic>A. veronii</italic>-V1 (<italic>aac(6’)-Ib, tetE, qnrS, IntI1</italic>), <italic>A</italic>. <italic>salmonicida</italic>–SL10 (<italic>IntI1, blaCTX, aac(3’)-Ib, aac(6’)-Ib, qnrS</italic>), <italic>A. hydrophila</italic>–H13 (<italic>IntI1, blaTEM, qnrS, aac(6’)-Ib, strA-strB</italic>). However, neither the <italic>blaSHV</italic>, <italic>blaIMP</italic>, <italic>tetB</italic>, <italic>qnrA</italic>, <italic>qnrB</italic>, and <italic>aphAI-IAB</italic> genes nor class1 integrons were detected in any of the isolates. Discrepancies between phenotypic and genetic resistance traits were observed in some isolates. With respect to outcomes, scallops are proposed as a source of multi-drug resistant <italic>Aeromonas</italic> spp. that harbor antimicrobial resistant genes.</p></abstract>

Antimicrobial resistance properties of 32 Aeromonas strains isolated from fresh scallops (Patinopecten yessoensis (Jay)) marketed in Korea were assessed. All the Aeromonas spp., including A. salmonicida, were mesophilic and grew very well at 37°C. The isolates were tested for susceptibility to 19 antimicrobials belonging to eight antimicrobial classes. All isolates were multi-drug resistant, which means they were resistant to five or more antimicrobials. Higher resistance rates (≥ 50%) were observed for ampicillin, piperacillin, cephalothin, imipenem, meropenem, trimethoprim-sulfamethoxazole, tetracycline, oxytetracycline, and nalidixic acid while intermediate resistance was also determined. PCR assays revealed the presence of many antimicrobial resistance genes among the isolates in varying combinations. Among them, some isolates harbored higher numbers of resistant genes, e.g., A. veronii-V1 (aac(6’)-Ib, tetE, qnrS, IntI1), A. salmonicida–SL10 (IntI1, blaCTX, aac(3’)-Ib, aac(6’)-Ib, qnrS), A. hydrophila–H13 (IntI1, blaTEM, qnrS, aac(6’)-Ib, strA-strB). However, neither the blaSHV, blaIMP, tetB, qnrA, qnrB, and aphAI-IAB genes nor class1 integrons were detected in any of the isolates. Discrepancies between phenotypic and genetic resistance traits were observed in some isolates. With respect to outcomes, scallops are proposed as a source of multi-drug resistant Aeromonas spp. that harbor antimicrobial resistant genes.

Multi-drug resistant mesophilic aeromonads isolated from marketed scallops (<italic>Patinopecten yessoensis</italic>) harboring resistance genes

Fisheries & Aquatic Life

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Multi-drug resistant mesophilic aeromonads isolated from marketed scallops () harboring resistance genes

Antimicrobial resistance properties of 32 Aeromonas strains isolated from fresh scallops (Patinopecten yessoensis (Jay)) marketed in Korea were assessed. All...

Multi-drug resistant mesophilic aeromonads isolated from marketed scallops (Patinopecten yessoensis) harboring resistance genes

Online veröffentlicht: 11. Mai 2020

Seitenbereich: 1 - 10

Eingereicht: 20. Okt. 2019

Akzeptiert: 03. März 2020

DOI: https://doi.org/10.2478/aopf-2020-0001

Schlüsselwörter
spp., antimicrobials, multi-drug resistance, resistance genes, scallop

© 2020 Benthotage Chamara Jayasankha De Silva et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Multi-drug resistant mesophilic aeromonads isolated from marketed scallops (Patinopecten yessoensis) harboring resistance genes

Online veröffentlicht: 11. Mai 2020

Seitenbereich: 1 - 10

Eingereicht: 20. Okt. 2019

Akzeptiert: 03. März 2020

DOI: https://doi.org/10.2478/aopf-2020-0001

Schlüsselwörterspp., antimicrobials, multi-drug resistance, resistance genes, scallop

© 2020 Benthotage Chamara Jayasankha De Silva et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Schlüsselwörter
spp., antimicrobials, multi-drug resistance, resistance genes, scallop