<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>In this study, we investigated the dietary effects of dandelion root extract (DRE) on growth performance, body composition, serum biochemical parameters, intestinal and liver histology, and fish resistance against <italic>Lactococcus garvieae</italic> infection in the rainbow trout. In total, 540 rainbow trout fry (22.05±1.740 g and 13.03±0.470 cm) were used. Six experimental groups (control, 2.5 mL/kg (DRE-1), 5 mL/kg (DRE-2), 10 mL/kg (DRE-3), 20 mL/kg (DRE-4), and 40 mL/kg (DRE-5) dandelion root extract) with three replicates were formed. In each tank, 30 fish were placed and fed for 90 days, and the volume of water was adjusted to 80 L with a flow-through system. The results showed that the final fish weight, weight gain, specific growth rate, protein efficiency ratio, and protein deposition rate were significantly higher in the DRE-4 group, while the feed conversion ratio and fat deposition ratio were significantly lower compared to that in the control group (P&lt;0.05). The viscerosomatic index and the hepatosomatic index decreased in the experimental groups depending on the concentration of the extract used, while the condition factor was higher than that in the control (P&lt;0.05). The DRE significantly decreased glucose, cholesterol, triglyceride, low-density lipoprotein, alanine aminotransferase, and aspartate aminotransferase levels in blood serum in the fish from the experimental groups, but it significantly increased high-density lipoprotein, alkaline phosphatase, and total protein levels (P&lt;0.05). Similarly, the DRE positively affected intestinal histology by increasing the stratum compactum, submucosa, lamina propria, villi length, villi width, absorption area, tunica muscularis thickness, mucosal folds, and goblet cell count. In the liver, depending on the high-fat diet (crude lipid = 20%), vacuolization areas, hepatocellular degeneration areas, apoptotic hepatocyte nucleus, and necrotic areas recorded in the control group decreased or even disappeared completely in the extract groups. The results of the challenge test showed that adding 2.5 mL/kg of the DRE to the feed positively affected the disease resistance against <italic>Lactococcus garvieae</italic> infection. The results of the quadratic polynomial regression showed that an average level of 23.91±1.048 mL/kg of DRE would be suitable for optimum fish growth.</p>
</abstract>

In this study, we investigated the dietary effects of dandelion root extract (DRE) on growth performance, body composition, serum biochemical parameters, intestinal and liver histology, and fish resistance against Lactococcus garvieae infection in the rainbow trout. In total, 540 rainbow trout fry (22.05±1.740 g and 13.03±0.470 cm) were used. Six experimental groups (control, 2.5 mL/kg (DRE-1), 5 mL/kg (DRE-2), 10 mL/kg (DRE-3), 20 mL/kg (DRE-4), and 40 mL/kg (DRE-5) dandelion root extract) with three replicates were formed. In each tank, 30 fish were placed and fed for 90 days, and the volume of water was adjusted to 80 L with a flow-through system. The results showed that the final fish weight, weight gain, specific growth rate, protein efficiency ratio, and protein deposition rate were significantly higher in the DRE-4 group, while the feed conversion ratio and fat deposition ratio were significantly lower compared to that in the control group (P<0.05). The viscerosomatic index and the hepatosomatic index decreased in the experimental groups depending on the concentration of the extract used, while the condition factor was higher than that in the control (P<0.05). The DRE significantly decreased glucose, cholesterol, triglyceride, low-density lipoprotein, alanine aminotransferase, and aspartate aminotransferase levels in blood serum in the fish from the experimental groups, but it significantly increased high-density lipoprotein, alkaline phosphatase, and total protein levels (P<0.05). Similarly, the DRE positively affected intestinal histology by increasing the stratum compactum, submucosa, lamina propria, villi length, villi width, absorption area, tunica muscularis thickness, mucosal folds, and goblet cell count. In the liver, depending on the high-fat diet (crude lipid = 20%), vacuolization areas, hepatocellular degeneration areas, apoptotic hepatocyte nucleus, and necrotic areas recorded in the control group decreased or even disappeared completely in the extract groups. The results of the challenge test showed that adding 2.5 mL/kg of the DRE to the feed positively affected the disease resistance against Lactococcus garvieae infection. The results of the quadratic polynomial regression showed that an average level of 23.91±1.048 mL/kg of DRE would be suitable for optimum fish growth.

Dandelion Root Extract in Trout Feed and its Effects on the Physiological Performance of <italic>Oncorhynchus mykiss</italic> and Resistance to <italic>Lactococcus garvieae</italic> Infection

Annals of Animal Science

This work is licensed under the Creative Commons Attribution 4.0 International License.

Dandelion Root Extract in Trout Feed and its Effects on the Physiological Performance of  and Resistance to  Infection

In this study, we investigated the dietary effects of dandelion root extract (DRE) on growth performance, body composition, serum biochemical parameters,...

Dandelion Root Extract in Trout Feed and its Effects on the Physiological Performance of Oncorhynchus mykiss and Resistance to Lactococcus garvieae Infection

Online veröffentlicht: 23. Jan. 2024

Seitenbereich: 161 - 177

Eingereicht: 21. Feb. 2023

Akzeptiert: 01. Juni 2023

DOI: https://doi.org/10.2478/aoas-2023-0072

Schlüsselwörter
medicinal plant, serum biochemistry, histology,

© 2024 Özay Köse et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Dandelion Root Extract in Trout Feed and its Effects on the Physiological Performance of Oncorhynchus mykiss and Resistance to Lactococcus garvieae Infection

Online veröffentlicht: 23. Jan. 2024

Seitenbereich: 161 - 177

Eingereicht: 21. Feb. 2023

Akzeptiert: 01. Juni 2023

DOI: https://doi.org/10.2478/aoas-2023-0072

Schlüsselwörtermedicinal plant, serum biochemistry, histology,

© 2024 Özay Köse et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Schlüsselwörter
medicinal plant, serum biochemistry, histology,