1. bookVolumen 22 (2022): Heft 3 (July 2022)
Zeitschriftendaten
License
Format
Zeitschrift
eISSN
2300-8733
Erstveröffentlichung
25 Nov 2011
Erscheinungsweise
4 Hefte pro Jahr
Sprachen
Englisch
access type Uneingeschränkter Zugang

Zinc-binding proteins in stallion seminal plasma as potential sperm function regulators

Online veröffentlicht: 19 Jul 2022
Volumen & Heft: Volumen 22 (2022) - Heft 3 (July 2022)
Seitenbereich: 961 - 976
Eingereicht: 28 May 2021
Akzeptiert: 11 Jan 2022
Zeitschriftendaten
License
Format
Zeitschrift
eISSN
2300-8733
Erstveröffentlichung
25 Nov 2011
Erscheinungsweise
4 Hefte pro Jahr
Sprachen
Englisch
Abstract

Sperm functions may be influenced by seminal plasma (SP) proteins with affinity to zinc ions. The aim of the study was to isolate and characterise zinc-binding proteins (ZnBPs) from stallion SP using proteomic tools. Zinc-binding proteins were isolated from the SP of eight stallions by zinc-affinity chromatography. They were analysed in the SDS-PAGE system, and peptide extracts were prepared. Samples of ZnBPs isolated from stallion SP were injected onto the nLC-1000 nanoflow HPLC system coupled via a nano-electrospray ion source to the Orbitrap Elite FTMS mass spectrometer (Thermo Fisher Scientific). Raw MS data were analysed using MaxQuant software with label-free quantification (LFQ). Protein sequences were obtained from the UniProt database. Forty-seven proteins in ZnBPs were annotated in a gene ontology (GO) analysis. The LFQ intensity analysis of individual proteins revealed that ZnBPs comprised mainly clusterin (CLU, 27%), serin protease inhibitor F1 (SERPINF1, 13%), actin, cytoplasmic 1 (ACTB, 9%), nucleobindin 2 (NUCB2, 8%) and polymeric immunoglobulin receptor (PIGR, 6%). This is the first proteomic study to analyse ZnBPs in stallion SP. The present findings show that ZnBPs in stallion SP could play an important role in the regulation of sperm function.

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