<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>This study aimed to define potential markers that could determine the suitability of ejaculate for cryopreservation. Fresh semen from eleven boars (4–7 ejaculates/boar), regardless of their sperm motility, was subjected to a cryopreservation procedure. The sperm quality before and after freezing was assessed based on the sperm membrane permeability and acrosome integrity. The results showed that it was possible to effectively cryopreserve ejaculates below the accepted standards of 70–80% of fresh motile sperm and still obtain a high cryosurvival rate. Moreover, a significant correlation was found between the percentage of viable sperm with apoptotic-like changes, viable sperm with reacted acrosomes, and the cryosurvival rate. The proposed markers for assessing the quality of fresh semen could be used to predict the success of cryopreservation procedures.</p>
</abstract>

This study aimed to define potential markers that could determine the suitability of ejaculate for cryopreservation. Fresh semen from eleven boars (4–7 ejaculates/boar), regardless of their sperm motility, was subjected to a cryopreservation procedure. The sperm quality before and after freezing was assessed based on the sperm membrane permeability and acrosome integrity. The results showed that it was possible to effectively cryopreserve ejaculates below the accepted standards of 70–80% of fresh motile sperm and still obtain a high cryosurvival rate. Moreover, a significant correlation was found between the percentage of viable sperm with apoptotic-like changes, viable sperm with reacted acrosomes, and the cryosurvival rate. The proposed markers for assessing the quality of fresh semen could be used to predict the success of cryopreservation procedures.

A new sperm selection criterion for cryopreservation of boar semen

Department of Reproductive Biotechnology and Cryoconservation

Annals of Animal Science

This work is licensed under the Creative Commons Attribution 4.0 International License.

{"article-title":"A new sperm selection criterion for cryopreservation of boar semen"}

This study aimed to define potential markers that could determine the suitability of ejaculate for cryopreservation. Fresh semen from eleven boars (4–7...

A new sperm selection criterion for cryopreservation of boar semen

Online veröffentlicht: 08. Mai 2021

Seitenbereich: 513 - 525

Eingereicht: 30. Juni 2020

Akzeptiert: 16. Sept. 2020

DOI: https://doi.org/10.2478/aoas-2020-0095

Schlüsselwörter
boar, semen, cryopreservation, selection criterion

© 2020 Monika Trzcińska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

A new sperm selection criterion for cryopreservation of boar semen

Online veröffentlicht: 08. Mai 2021

Seitenbereich: 513 - 525

Eingereicht: 30. Juni 2020

Akzeptiert: 16. Sept. 2020

DOI: https://doi.org/10.2478/aoas-2020-0095

Schlüsselwörterboar, semen, cryopreservation, selection criterion

© 2020 Monika Trzcińska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Schlüsselwörter
boar, semen, cryopreservation, selection criterion