Surgical procedure for acellular vascular xenografts testing in sheep carotid artery
Online veröffentlicht: 02. Okt. 2024
Seitenbereich: 118 - 121
Eingereicht: 15. Juli 2024
Akzeptiert: 20. Juli 2024
DOI: https://doi.org/10.2478/amma-2024-0024
Schlüsselwörter
© 2024 Marius Mihai Harpa et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.
Objective
The objective of this study is to demonstrate the safety and reproducibility of our surgical technique for implanting decellularized vascular xenografts in the carotid artery of sheep.
Methods
Acellular porcine carotid arteries were implanted as interposition xenografts in seven sheep. An intravascular shunt was used for cerebral protection, and a flowmeter was utilized to assess graft performance.
Results
There were no intraoperative deaths or postoperative neurological complications observed. Acute graft thrombosis occurred in one sheep during surgery, but was successfully managed with thrombectomy to restore blood flow. Post-implantation flowmetry and Doppler ultrasound confirmed graft functionality.
Conclusions
Our study demonstrates the successful application of our surgical method for implanting decellularized vascular xenografts in the carotid artery of sheep. The implanted grafts maintained patency, normal blood flow, and favorable wound healing and neurological outcomes post-surgery.