Experimental group number | Type of therapy applied | Route of cells delivery | Dosage of saline/number of cells injected | Number of rats per group |
---|---|---|---|---|
Group 1 | Saline | Intraosseous | 0.1 mL of saline | |
Group 2 | alloBMT | Intraosseous | 80 × 106 cells | |
Group 3 | DRCC | Intraosseous | 4–6 × 106 cells | |
Group 4 | alloBMT + DRCC | Intraosseous | 80 × 106 cells + 4–6 × 106 cells | |
Total number of rats ( |
Kidney | Skin | Small intestine |
---|---|---|
Tubulointerstitial inflammation, basement membrane nephropathy, microangiopathy, fibrinoid necrosis (segmental or global), interstitial fibrosis, tubular necrosis | Vacuolar degeneration, epidermal necrosis, lichenification, inflammation of the skin appendages, non-specific lymphocytic dermatitis, apoptosis and dermal keratosis, fibrosis of the dermis, hyperkeratosis of the epidermis, acanthosis, spongiotic dermatitis with marked spongiosis | Non-specific mucositis, gland damage, mucosal damage, the presence of crypt distortion, active inflammation, Paneth cell metaplasia, degree of mucosal fibrosis, apoptotic changes within crypts, the presence of intestinal endothelial lymphocytes |
Degree of GvHD occurrence (score) | No GvHD (0) | GvHD possible (1) | Consistent with GvHD (2) | Definite GvHD (3) |
---|---|---|---|---|
Kidney | No deviation | Presence of <2 small criteria | Presence of ≥2 small criteria without the presence of basement membrane nephropathy | Presence of basement membrane nephropathy |
Skin | Presence of one small criterion or the absence of deviations | Presence of ≥2 small criteria or the presence of apoptosis | Presence of lichenification, dermal fibrosis, spongiosis without apoptosis, or presence of apoptosis with small criteria but excluding lichenification and spongiosis | Occurrence of apoptosis with lichenification and acanthosis or spongiosis |
Small intestine | Presence of one small criterion or the absence of deviations | Presence of ≥2 small criteria or small criteria without the presence of crypt apoptosis | Presence of ≥2 small criteria in the presence of 2–5 apoptotic crypts | Presence of >6 apoptotic crypts with at least one small criterion |
Experimental groups | Activity in the cage (0–3) | Body posture (0–3) | Fur coverage (0–3) | Overall physical condition total score (0–9) | Blood in stool (0–1) | Diarrhea incidence (0–1) | Digestive distress condition total score (0–2) |
---|---|---|---|---|---|---|---|
Saline control | 0.96 ± 0.453 |
0.02 ± 0.026 | 0.70 ± 0.089 | 1.68 | 0.18 ± 0.187 | 0.16 ± 0.118 |
0.34 |
alloBMT | 0.13 ± 0.031 |
0.34 ± 0.227 |
1.15 ± 0.273 |
1.62 | 0.19 ± 0.194 |
0.09 ± 0.025 | 0.28 |
DRCC | 0.25 ± 0.303 | 0.02 ± 0.027 | 0.51 ± 0.118 |
0.78 | 0.06 ± 0.025 | 0.00 ± 0.009 |
0.06 |
alloBMT + DRCC | 0.29 ± 0.302 | 0.00 ± 0.000 |
0.60 ± 0.094 | 0.89 | 0.07 ± 0.023 |
0.08 ± 0.027 | 0.15 |
Activity in the cage |
0- Very active animal, moving in the cage, typical gait 1- Slightly decreased degree of activity, gait mildly disturbed 2- Animal moving very slowly, gait severely disturbed 3- Animal reluctant to move or lack of any movement |
0–3 points |
Body posture |
0- Normal body posture 1- Slightly hunched over 2- Moderately hunched over 3- Very hunched over |
0–3 points |
Fur coverage |
0- Normal fur condition 1- No fur loss and spiky fur 2- Moderate fur loss and raised fur 3- Significant fur loss and bristling fur |
0–3 points |
Overall physical condition total score = 0–9 points | ||
Blood in stool incidence |
0- Absence of blood in stool 1- Presence of blood in stool |
0–1 point |
Diarrhea incidence |
0- Absence of diarrhea, normal stool consistency 1- Presence of diarrhea |
0–1 point |
Digestive distress condition total score = 0–2 points |