Uneingeschränkter Zugang

Effects of naringin and valproate interaction on liver steatosis and dyslipidaemia parameters in male C57BL6 mice

David Jutrić
David Jutrić
, 
Domagoj Đikić
Domagoj Đikić
, 
Almoš Boroš
Almoš Boroš
, 
Dyna Odeh
Dyna Odeh
, 
Sandra Domjanić Drozdek
Sandra Domjanić Drozdek
, 
Romana Gračan
Romana Gračan
, 
Petar Dragičević
Petar Dragičević
, 
Irena Crnić
Irena Crnić
 und 
Irena Landeka Jurčević
Irena Landeka Jurčević
   | 07. Apr. 2022
Archives of Industrial Hygiene and Toxicology's Cover Image
Über diesen Artikel
Vorheriger Artikel
Nächster Artikel
Zitieren
Article Category: Original article
Online veröffentlicht: 07. Apr. 2022
Seitenbereich: 71 - 82
Eingereicht: 01. Okt. 2021
Akzeptiert: 01. Feb. 2022
DOI: https://doi.org/10.2478/aiht-2022-73-3608
© 2022 David Jutrić, Domagoj Đikić, Almoš Boroš, Dyna Odeh, Sandra Domjanić Drozdek, Romana Gračan, Petar Dragičević, Irena Crnić, Irena Landeka Jurčević, published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1

Serum lipids and lipoproteins in control mice and mice treated with daily doses of 150 mg/kg bw valproate, 25 mg/kg bw naringin, and their combination for 10 days. The numbers above bars represent statistically significant differences (p<0.05) between specific groups
Serum lipids and lipoproteins in control mice and mice treated with daily doses of 150 mg/kg bw valproate, 25 mg/kg bw naringin, and their combination for 10 days. The numbers above bars represent statistically significant differences (p<0.05) between specific groups

Figure 2

Lipid steatosis stained with Oil Red in the liver of control mice (A) and mice treated with daily doses of 150 mg/kg bw valproate (B), 25 mg/ kg bw naringin (C), and their combination (D) for 10 days
Lipid steatosis stained with Oil Red in the liver of control mice (A) and mice treated with daily doses of 150 mg/kg bw valproate (B), 25 mg/ kg bw naringin (C), and their combination (D) for 10 days

Figure 3

Liver lipid metabolism regulators in control mice and mice treated with daily doses of 150 mg/kg bw valproate, 25 mg/kg bw naringin, and their combination for 10 days. The numbers above bars represent statistically significant differences (p<0.05) between specific groups
Liver lipid metabolism regulators in control mice and mice treated with daily doses of 150 mg/kg bw valproate, 25 mg/kg bw naringin, and their combination for 10 days. The numbers above bars represent statistically significant differences (p<0.05) between specific groups

Figure 4

Liver tissue oxidative stress parameters in control mice and mice treated with daily doses of 150 mg/kg bw valproate, 25 mg/kg bw naringin, and their combination for 10 days. The numbers above bars represent statistically significant differences (p<0.05) between specific groups. MDA – malondialdehyde
Liver tissue oxidative stress parameters in control mice and mice treated with daily doses of 150 mg/kg bw valproate, 25 mg/kg bw naringin, and their combination for 10 days. The numbers above bars represent statistically significant differences (p<0.05) between specific groups. MDA – malondialdehyde

Figure 5

Liver tissue antioxidants in control mice and mice treated with daily doses of 150 mg/kg bw valproate, 25 mg/kg bw naringin, and their combination for 10 days. The numbers above bars represent statistically significant differences (p<0.05) between specific groups. SOD – total superoxide dismutase activity; CAT – catalase activity; GSH – reduced glutathione
Liver tissue antioxidants in control mice and mice treated with daily doses of 150 mg/kg bw valproate, 25 mg/kg bw naringin, and their combination for 10 days. The numbers above bars represent statistically significant differences (p<0.05) between specific groups. SOD – total superoxide dismutase activity; CAT – catalase activity; GSH – reduced glutathione

Figure 6

Transcription factor Nrf2 in the liver of control mice and mice treated with daily doses of 150 mg/kg bw valproate, 25 mg/kg bw naringin, and their combination for 10 days. No significant differences were observed between the groups
Transcription factor Nrf2 in the liver of control mice and mice treated with daily doses of 150 mg/kg bw valproate, 25 mg/kg bw naringin, and their combination for 10 days. No significant differences were observed between the groups

Serum biochemistry parameters in control mice and mice treated with daily doses of 150 mg/kg bw valproate, 25 mg/kg bw naringin, and their combination for 10 days

Parameter Control Valproate Naringin Valproate+Naringin
Glucose (mmol/L)
Mean ± SD 5.9±0.9 12.7±1.4a 14.0±4.2a,b 17.8±1.5b
Median 5.8 12.6 13.4 16.3
Range 5.0–7.2 11.5–14.3 9.8–19.3 9.8–19.9
Amylase activity (U/L)
Mean ± SD 365.3±110.6 555.0±520.4a 371.8±23.8 455.8±102.4b
Median 331.0 355.0 373.5 459.0
Range 273.0–526.0 184.0–1326.0 342.0–398.0 356.0–549.0
Lactate dehydrogenase (LDH) activity (U/L)
Mean ± SD 264.8±29.2 316.4±40.3a 382.0±97.8a 498.5±324.2a
Median 257.0 331.0 392.0 531.5
Range 240.0–305.0 250.0–348.0 263.0–518.0 122.0–809.0
Creatinine (mg/L)
Mean ± SD 22.8±3.3 31.3±7.8a 27.5±10.5 42.8±3.6b
Median 22.5 29.0 25.0 43.0
Range 19.0–27.0 25.0–42.0 18.0–42.0 24.0–56.0
Bound urea nitrogen (BUN) (mmol/L)
Mean ± SD 6.0±1.3 10.2±7.6a 6.9±1.1 10.0±3.4a
Median 5.7 8.5 7.0 8.5
Range 4.8–7.7 3.0–21.0 5.4–8.0 7.9–15.0
Bilirubin (μmol/L)
Mean ± SD 5.3±0.2 5.6±0.4 5.1±0.2 5.1±0.2
Median 5.3 5.8 5.0 5.1
Range 5.0–5.5 5.1–5.9 5.0–5.4 5.0–5.4
Alkaline phosphatase (ALP) activity (U/L)
Mean ± SD 46.3±6.3 44.5±3.1 32.0±14.1 42.0±2.2
Median 47.3 45.5 38.5 41.5
Range 40.0–55.0 40.0–47.0 11.0–40.0 40.0–45.0
Total proteins (mg/L)
Mean ± SD 37.3±5.0 34.5±10.2 40.5±5.9 38.1±0.9
Median 37.5 37.5 40.0 38.0
Range 32.0–42.0 20.0–43.0 37.0–49.0 37.0–39.0
Albumin (mg/L)
Mean ± SD 27.0±2.4 34.3±6.5 36.0±5.0 31.0±0.8
Median 26.5 36.0 36.0 30.0
Range 25.0–30.0 25.0–40.0 30.0–42.0 29.0–33.0
eISSN:
1848-6312
Sprachen:
Englisch, Slovenian
Zeitrahmen der Veröffentlichung:
4 Hefte pro Jahr
Fachgebiete der Zeitschrift:
Medizin, Vorklinische Medizin, Grundlagenmedizin, andere
Zeitschrift RSS Feed