Uneingeschränkter Zugang

Can colistin susceptibility results determined in multidrug resistant Gram-negative bacteria be used for polymyxin B?

Rıza Adaleti
Rıza Adaleti
, 
Nilgün Kansak
Nilgün Kansak
, 
Şeyma Çalık
Şeyma Çalık
, 
Neslihan Arıcı
Neslihan Arıcı
, 
Seniha Şenbayrak
Seniha Şenbayrak
 und 
Sebahat Aksaray
Sebahat Aksaray
   | 14. Nov. 2023
Postępy Higieny i Medycyny Doświadczalnej's Cover Image
Über diesen Artikel
Vorheriger Artikel
Nächster Artikel
Zitieren
Article Category: Original Study
Online veröffentlicht: 14. Nov. 2023
Seitenbereich: 125 - 132
Eingereicht: 31. Jan. 2023
Akzeptiert: 17. Aug. 2023
DOI: https://doi.org/10.2478/ahem-2023-0017
Schlüsselwörter
© 2023 Rıza Adaleti et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

MIC values (μg/ml) for colistin and polymyxin B in P.aeruginosa and A. baumannii strains (n = 117)Grey font numbers indicate the number of strains with equivalent MICs for colistin and polymyxin B.

Colistin (mg/L) >=256 2A
128 1A
64 1P+1A
32 1A
16 1A 1P+1A
8 1P+2A 1P 2P
4 3A 2P+2A 2P+1A 5P+1A 1A
2 1A 1P+5A 10P+8A 16P+9A 3P
1 1A 4A 12P+4A 1P+1A
0.5 1P+2A 1P+2A
0.25 2P 1P
0.25 0.5 1 2 4 8 16 32 64 128 >=256
Polymyxin B (mg/L)

MIC values (μg/ml) for colistin and polymyxin B in E. coli and K. pneumoniae bacteria (n = 151)Grey font numbers indicate the number of strains with equivalent MICs for colistin and polymyxin B

Colistin (mg/L) >=256 1K
128 1K 2K 1K
64 4K 9K 5K
32 3K 12K 8K 1K
16 4K 4K
8 1K+1E 1K 1K
4 1K+3E 1K+8E 1K+3E 1K
2 2k 2K 5K+9E 2K+7E 2E
1 1k 2K+2E 2K+4E 1K+9E 5E
0.5 2k 3K+2E 1K 2K+2E 1E
<=0.25 3k+1E 2K
0.25 0.5 1 2 4 8 16 32 64 128 >=256
Polymyxin B (mg/L)

MIC values and interpretations of colistin and polymyxin B against Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Acinetobacter baumannii strains

Bac. Col. MIC Interp. Poly. MIC Int. Bac. Col. MIC Interp. Poly. MIC Interp. Bac. Col. MIC Int. Poly. MIC Int. Bac. Col. MIC Int. Poly. MIC Int. Bac. Col. MIC Int. Poly. MIC Int.
E1 1 I 2 I K1 1 I 1 I K64 64 R 64 R P1 2 I 1 I A1 4 R 0.5 I
E2 1 I 4 R K2 1 I 1 I K65 64 R 32 R P2 4 R 1 I A2 4 R 2 I
E3 4 R 1 I K3 1 I 2 I K66 64 R 32 R P3 1 I 1 I A3 8 R 1 I
E4 2 I 1 I K4 1 I 0.5 I K67 64 R 32 R P4 1 I 1 I A4 256 R 128 R
E5 1 I 2 I K5 1 I 0.25 I K68 64 R 16 R P5 2 I 1 I A5 2 I 0.5 I
E6 2 I 2 I K6 1 I 0.5 I K69 64 R 32 R P6 4 R 2 I A6 4 R 4 R
E7 1 I 4 R K7 2 I 1 I K70 64 R 16 R P7 2 I 4 R A7 2 I 1 I
E8 1 I 2 I K8 2 I 1 I K71 64 R 32 R P8 1 I 2 I A8 0.5 I 0.5 I
E9 1 I 2 I K9 2 I 1 I K72 128 R 64 R P9 1 I 1 I A9 2 I 0.25 I
E10 4 R 2 I K10 2 I 0.5 I K73 128 R 64 R P10 1 I 1 I A10 2 I 2 I
E11 1 I 1 I K11 2 I 1 I K74 128 R 32 R P11 1 I 1 I A11 2 I 1 I
E12 1 I 2 I K12 2 I 0.25 I K75 128 R 128 R P12 2 I 1 I A12 16 R 4 R
E13 2 I 2 I K13 2 I 0.25 I K76 32 R 16 R P13 2 I 1 I A13 2 I 2 I
E14 1 I 2 I K14 2 I 0.5 I K77 0.25 I 0.5 I P14 1 I 1 I A14 0.5 I 0.5 I
E15 0.5 I 2 I K15 2 I 1 I K78 0.25 I 0.5 I P15 4 R 1 I A15 2 I 1 I
E16 0.5 I 2 I K16 2 I 2 I K79 0.25 I 0.25 I P16 2 I 1 I A16 2 I 1 I
E17 1 I 1 I K17 2 I 2 I K80 0.25 I 0.25 I P17 16 R 16 R A17 1 I 0.5 I
E18 1 I 0.5 I K18 4 R 8 R K81 0.25 I 0.25 I P18 2 I 1 I A18 2 I 2 I
E19 2 I 1 I K19 4 R 1 I K82 0.5 I 0.25 I P19 2 I 2 I A19 1 I 2 I
E20 4 R 2 I K20 4 R 4 R K83 0.5 I 0.5 I P20 1 I 1 I A20 2 I 2 I
E21 2 I 2 I K21 4 R 2 I K84 0.5 I 0.5 I P21 1 I 1 I A21 0.5 I 0.25 I
E22 2 I 1 I K22 8 R 2 I K85 0.5 I 1 I P22 1 I 1 I A22 2 I 2 I
E23 1 I 2 I K23 8 R 8 R K86 0.5 I 0.5 I P23 1 I 1 I A23 2 I 1 I
E24 1 I 4 R K24 8 R 4 R K87 0.5 I 0.25 I P24 8 R 2 I A24 1 I 1 I
E25 4 R 2 I K25 16 R 8 R K88 0.5 I 2 I P25 8 R 4 R A25 1 I 1 I
E26 2 I 4 R K26 16 R 16 R K89 0.5 I 2 I P26 8 R 4 R A26 2 I 2 I
E27 1 I 4 R K27 16 R 16 R K90 32 R 32 R P27 2 I 0.5 I A27 4 R 1 I
E28 2 I 1 I K28 16 R 16 R K91 256 R 128 R P28 2 I 2 I A28 256 R 128 R
E29 8 R 2 I K29 16 R 8 R K92 16 R 8 R P29 8 R 1 I A29 16 R 16 R
E30 4 R 2 I K30 16 R 16 R P30 2 I 1 I A30 2 I 1 I
E31 1 I 4 R K31 16 R 8 R P31 2 I 1 I A31 1 I 1 I
E32 1 I 2 I K32 32 R 8 R P32 1 I 1 I A32 128 R 64 R
E33 2 I 2 I K33 32 R 16 R P33 64 R 64 R A33 1 I 0.5 I
E34 0.5 I 4 R K34 32 R 16 R P34 1 I 1 I A34 32 R 32 R
E35 4 R 4 R K35 32 R 64 R P35 0.25 I 1 I A35 2 I 1 I
E36 1 I 1 I K36 32 R 32 R P36 2 I 1 I A36 64 R 64 R
E37 4 R 2 I K37 32 R 32 R P37 2 I 2 I A37 1 I 1 I
E38 2 I 1 I K38 32 R 16 R P38 2 I 1 I A38 0.5 I 0.25 I
E39 1 I 2 I K39 32 R 32 R P39 0.5 I 0.5 I A39 1 I 0.5 I
E40 4 R 2 I K40 32 R 16 R P40 2 I 2 I A40 1 I 0.25 I
E41 4 R 2 I K41 32 R 32 R P41 0.25 I 0.25 I A41 4 R 8 R
E42 2 I 2 I K42 32 R 8 R P42 0.25 I 0.25 I A42 2 I 0.5 I
E43 4 R 4 R K43 32 R 16 R P43 0.5 I 0.25 I A43 2 I 0.5 I
E44 2 I 2 I K44 32 R 8 R P44 4 R 2 I A44 4 R 1 I
E45 4 R 4 R K45 32 R 16 R P45 4 R 4 R A45 4 R 0.5 I
E46 2 I 4 R K46 32 R 32 R P46 2 I 4 R A46 2 I 0.5 I
E47 1 I 0.5 I K47 32 R 16 R P47 2 I 2 I A47 4 R 0.5 S
E48 2 I 1 I K48 32 R 16 R P48 2 I 2 I A48 2 I 0.5 I
E49 1 I 1 I K49 32 R 16 R P49 2 I 2 I A49 8 R 1 I
E50 4 R 2 I K50 32 R 16 R P50 2 I 2 I A50 2 I 2 I
E51 0.25 I 0.25 I K51 32 R 32 R P51 2 I 2 I A51 2 I 2 I
E52 0.5 I 0.5 I K52 32 R 32 R P52 4 R 4 R A52 1 I 0.5 I
E53 2 I 2 I K53 32 R 16 R P53 4 R 4 R A53 2 I 2 I
E54 4 R 1 I K54 64 R 32 R P54 2 I 2 I A54 2 I 1 I
E55 0.5 I 0.5 I K55 64 R 32 R P55 4 R 4 R
E56 2 I 1 I K56 64 R 16 R P56 2 I 2 I
E57 2 I 1 I K57 64 R 32 R P57 2 I 2 I
E58 2 I 1 I K58 64 R 16 R P58 2 I 2 I
E59 4 R 1 I K59 64 R 64 R P59 2 I 2 I
K60 64 R 64 R P60 2 I 2 I
K61 64 R 64 R P61 2 I 2 I
K62 64 R 32 R P62 2 I 4 R
K63 64 R 64 R P63 4 R 4 R

Differences in MIC values between colistin and polymyxin B among tested isolates

Organisms (n) Polymyxin B more potent by n (%) of strains Same MIC Colistin more potent by n (%) of strains
3 dilution 2 dilution 1 dilution n (%) 1 dilution 2 dilution 3 dilution
E.coli (59) 0 4 (6.8) 19 (32.2) 17 (28.8) 11 (18.6) 7 (11.9) 1 (1.7)
K. pneumonia (92) 2 (2.2) 13 (14.1) 39 (42.4) 30 (32.6) 6 (6.5) 2 (2.2) 0
P. aeruginosa (63) 1 (1.6) 4 (6.3) 15 (23.8) 38 (60.3) 4 (6.3) 1 (1.6) 0
A. baumannii (54) 6 (11.1) 9 (16.7) 18 (33.3) 19 (35.2) 2 (3.7) 0 0
Total 268 9 (3.4) 30 (11.2) 91 (34.0) 104 (38.8) 23 (8.6) 10 (3.7) 1 (0.4)

Colistin and polymyxin B evaluation criteria for Enterobacterales, P. aeruginosa and A. baumannii according to CLSI and EUCAST criteria

CLSI Susceptible (μg/mL) Intermediate(μg/mL) Resistant(μg/mL)
Colistin ≤2 ≥4
Polymyxin B ≤2 ≥4
EUCAST Susceptible (mg/L) Intermediate (mg/L) Resistant (mg/L) ECOFF (mg/L)
Colistin* ≤2 ≥4
Colistin** ≤4 ≥8
Polymyxin B* 2
Polymyxin B** 4

The essential agreement, categorical agreement rates of polymyxin B and resistance rates of colistin and polymyxin B in studied bacteria

Colistin Polymyxin B
Bacteria EA n (%) CA n (%) I n (%) R n (%) I n (%) R n (%)
E. coli 47 (79.7) 39 (66.1) 44 (74.6) 15 (25.4) 48 (81.4) 11 (18.6)
K. pneumoniae 75 (81.5) 89 (96.7) 30 (32.6) 62 (67.4) 33 (35.9) 59 (64.1)
P. aeruginosa 57 (90.5) 54 (85.7) 48 (76.2) 15 (23.8) 51 (81.0) 12(19.0)
A. baumannii 39 (72.2) 46 (85.2) 37 (68.5) 17 (31.5) 45 (83.3) 9 (16.7)
Total 218 (81.3) 228 (85.1) 159 (59.3) 109 (40.7) 177 (66.0) 91 (34.0)

Categorical agreement rates changing according to susceptibility status for colistin-polymyxin B intermediate–resistant isolates

Strains Colistin Polymyxin B % of Categorical agreement
Escherichia coli (I) 44 36 81.8
Escherichia coli(R) 15 3 20
Klebseilla pneumoniae(I) 30 30 100
Klebseilla pneumoniae(R) 62 59 95.2
Escherichia coli+ Klebseilla pneumoniae(I) 74 66 89.2
Escherichia coli+ Klebseilla pneumoniae(R) 77 62 80.5
Pseudomonas aeruginoa(I) 48 45 93.8
Pseudomonas aeruginoa(R) 15 9 60
Acinetobacter baumannii(I) 37 37 100
Acinetobacter baumannii (R) 17 9 52.9
Pseudomonas aeruginoa +Acinetobacter baumannii(I) 85 82 96.5
Pseudomonas aeruginoa +Acinetobacter baumannii(R) 32 18 56.3
eISSN:
1732-2693
Sprache:
Englisch
Zeitrahmen der Veröffentlichung:
Volume Open
Fachgebiete der Zeitschrift:
Biologie, Molekularbiologie, Mikrobiologie und Virologie, Medizin, Vorklinische Medizin, Grundlagenmedizin, Immunologie
Zeitschrift RSS Feed