<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec><h3>Introduction</h3>
<p>P-element induced wimpy testis-like 2 (Piwil2) is in the Piwi gene family. Piwil2 has important roles in the self-renewal mechanism of stem cell induction and progression of numerous types of human malignancies such as lung, breast, colon, prostate, and cervical cancers. Glutathione <italic>S</italic>-transferase (GST) acts as detoxification in cancer metabolism. This study aimed to investigate the effects of the stem cell protein Piwil2 on MCF10A and MCF-7 at the GST activity levels.</p>
</sec>
<sec><h3>Materials/Methods</h3>
<p>MCF-7/Piwil2 and MCF10A/Piwil2, transfected with a plasmid carrying the Piwil2 gene, and non-transfected MCF-7 and MCF10A were cultured in a complete DMEM/F12 medium. GST A1 and P1 activity was determined in these cell lines using as substrates CDNB, EA respectively.</p>
</sec>
<sec><h3>Results</h3>
<p>According to experimental results, GST P1 activity decreased in the MCF-7/Piwil2 cells as compared with the non-transfected MCF-7 cells, however, MCF-7/Piwil2 cells demonstrated increases in GST A1 (total GST) activity. The statistically significant differences were found for the comparison of non-transfected MCF-7 and MCF-7/Piwil2 (p&lt;0,0001), for GST enzyme activities by using CDNB and EA as substrates. These results were the same for the MCF10A cell line.</p>
</sec>
<sec><h3>Discussion</h3>
<p>It is shown for the first time that transfection studies may affect GST activity at the cellular mechanism level. The study contributes to determining the effect of transfection on GST isoenzymes and also how the Piwil2 gene may affect GST activity in the stem cell line.</p>
</sec>
</abstract>

Introduction
P-element induced wimpy testis-like 2 (Piwil2) is in the Piwi gene family. Piwil2 has important roles in the self-renewal mechanism of stem cell induction and progression of numerous types of human malignancies such as lung, breast, colon, prostate, and cervical cancers. Glutathione S-transferase (GST) acts as detoxification in cancer metabolism. This study aimed to investigate the effects of the stem cell protein Piwil2 on MCF10A and MCF-7 at the GST activity levels.

Materials/Methods
MCF-7/Piwil2 and MCF10A/Piwil2, transfected with a plasmid carrying the Piwil2 gene, and non-transfected MCF-7 and MCF10A were cultured in a complete DMEM/F12 medium. GST A1 and P1 activity was determined in these cell lines using as substrates CDNB, EA respectively.

Results
According to experimental results, GST P1 activity decreased in the MCF-7/Piwil2 cells as compared with the non-transfected MCF-7 cells, however, MCF-7/Piwil2 cells demonstrated increases in GST A1 (total GST) activity. The statistically significant differences were found for the comparison of non-transfected MCF-7 and MCF-7/Piwil2 (p<0,0001), for GST enzyme activities by using CDNB and EA as substrates. These results were the same for the MCF10A cell line.

Discussion
It is shown for the first time that transfection studies may affect GST activity at the cellular mechanism level. The study contributes to determining the effect of transfection on GST isoenzymes and also how the Piwil2 gene may affect GST activity in the stem cell line.

Expression and biochemical significance of Piwil2 in stem cell lines

Postępy Higieny i Medycyny Doświadczalnej

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

{"article-title":"Expression and biochemical significance of Piwil2 in stem cell lines"}

Introduction
P-element induced wimpy testis-like 2 (Piwil2) is in the Piwi gene family. Piwil2 has important roles in the self-renewal mechanism of stem cell...

Expression and biochemical significance of Piwil2 in stem cell lines

Article Category: Original Study

Online veröffentlicht: 02. Apr. 2022

Seitenbereich: 97 - 103

Eingereicht: 20. Apr. 2021

Akzeptiert: 23. Sept. 2021

DOI: https://doi.org/10.2478/ahem-2022-0009

Schlüsselwörter
cancer stem cell, ethacrynic acid, glutathione -transferase, piwil2

© 2022 Dilek Kaan, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Expression and biochemical significance of Piwil2 in stem cell lines

Article Category: Original Study

Online veröffentlicht: 02. Apr. 2022

Seitenbereich: 97 - 103

Eingereicht: 20. Apr. 2021

Akzeptiert: 23. Sept. 2021

DOI: https://doi.org/10.2478/ahem-2022-0009

Schlüsselwörtercancer stem cell, ethacrynic acid, glutathione -transferase, piwil2

© 2022 Dilek Kaan, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Schlüsselwörter
cancer stem cell, ethacrynic acid, glutathione -transferase, piwil2