<abstract xmlns="http://www.w3.org/1999/xhtml"><bold>Aim:</bold> The aim is to identify the possible changes in the expression of genes, that regulate calcium homeostasis in cardiomyocytes in diabetes mellitus.<bold>Methods:</bold> Male Wistar rats were randomized into two experimental protocols: short-term 5-days streptozotocin-induced diabetes protocol with 20 weeks old animals at the end of the protocol (total N = 20) and long-term 4-weeks protocol with 18 weeks of age at the end of the protocol (total N = 38). 50 mg/kg of streptozotocin (STZ) was administered in both protocols by a single intraperitoneal injection in 0,1M citrate buffer (pH = 4.5). Control group (CON) received only vehiculum. Gene expressions in samples of left heart ventricle were measured by RT-qPCR method.<bold>Results:</bold> The expression of SERCA2a in short-term protocol was decreased. In long-term protocol, decreased SERCA2a, TRPC4 and TRPC6 mRNA levels were observed (*p &lt; 0.05). SERCA2a and TRPC4 mRNA levels exhibited statistical monotonic correlation in STZ-treated group in long-term protocol.<bold>Conclusions:</bold> In diabetes mellitus, the calcium homeostasis in cardiomyocytes is altered and there could be a relation between alteration of internal sarcoplasmatic stores and store-operated calcium entry.</abstract>

Aim: The aim is to identify the possible changes in the expression of genes, that regulate calcium homeostasis in cardiomyocytes in diabetes mellitus.Methods: Male Wistar rats were randomized into two experimental protocols: short-term 5-days streptozotocin-induced diabetes protocol with 20 weeks old animals at the end of the protocol (total N = 20) and long-term 4-weeks protocol with 18 weeks of age at the end of the protocol (total N = 38). 50 mg/kg of streptozotocin (STZ) was administered in both protocols by a single intraperitoneal injection in 0,1M citrate buffer (pH = 4.5). Control group (CON) received only vehiculum. Gene expressions in samples of left heart ventricle were measured by RT-qPCR method.Results: The expression of SERCA2a in short-term protocol was decreased. In long-term protocol, decreased SERCA2a, TRPC4 and TRPC6 mRNA levels were observed (*p < 0.05). SERCA2a and TRPC4 mRNA levels exhibited statistical monotonic correlation in STZ-treated group in long-term protocol.Conclusions: In diabetes mellitus, the calcium homeostasis in cardiomyocytes is altered and there could be a relation between alteration of internal sarcoplasmatic stores and store-operated calcium entry.

Deteriorated gene expression of selected calcium transporters in streptozotocin-induced diabetic hearts of Wistar rats

Faculty of Pharmacy, Department of Pharmacology and Toxicology

European Pharmaceutical Journal

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Aim: The aim is to identify the possible changes in the expression of genes, that regulate calcium homeostasis in cardiomyocytes in diabetes mellitus.Methods:...

Deteriorated gene expression of selected calcium transporters in streptozotocin-induced diabetic hearts of Wistar rats

Online veröffentlicht: 07. Dez. 2018

Seitenbereich: 1 - 3

Eingereicht: 28. Feb. 2018

Akzeptiert: 04. Juni 2018

DOI: https://doi.org/10.2478/afpuc-2018-0005

Schlüsselwörter
TRPC, diabetes, SERCA2a, calcium, heart ventricles

© 2018 Dragún M. et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Deteriorated gene expression of selected calcium transporters in streptozotocin-induced diabetic hearts of Wistar rats

Online veröffentlicht: 07. Dez. 2018

Seitenbereich: 1 - 3

Eingereicht: 28. Feb. 2018

Akzeptiert: 04. Juni 2018

DOI: https://doi.org/10.2478/afpuc-2018-0005

SchlüsselwörterTRPC, diabetes, SERCA2a, calcium, heart ventricles

© 2018 Dragún M. et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Schlüsselwörter
TRPC, diabetes, SERCA2a, calcium, heart ventricles