<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Bee venom (BV) is a rich source of secondary metabolites from honeybees (<italic>Apis mellifera</italic> L.). It contains a variety of bioactive ingredients including peptides, proteins, enzymes, and volatile metabolites. This study investigated the antibacterial effects of the bee venom obtained from honey bees (<italic>Apis mellifera</italic> L.) against bacterial fish pathogens, such as <italic>Lactococcus garvieae</italic> (Lg1, Lg2, Lg3), <italic>Vibrio anguillarum</italic> (Va1, Va2, Va3), <italic>Yersinia ruckeri</italic> (Yr1, Yr2, Yr3), and <italic>Aeromonas hydrophila (</italic>Ah1, Ah2, Ah3) and the expression levels on the antibiotic resistance genes <italic>hly</italic> and <italic>fbp (hemolysin</italic> and <italic>fibronectin-binding prot)</italic> of them. It was determined that bee venom had an antibacterial effect against <italic>L. garvieae, L. anguillarum</italic>, and <italic>Y. ruckeri</italic> strains, while it had no effect only against Ah3 and Ah2 bacterial strains. As staded by the gene expression of <italic>hly (hemolysin)</italic> and <italic>fbp</italic> (<italic>fibronectin-binding protein</italic>), among the antibiotic resistance genes the effect levels of bee venom on bacterial species varied, although it affected antibiotic resistance and gene expression level in all bacteria. It was revealed that the expression level was the highest for <italic>V. anguillarum</italic> strains, whereas it was below the control group for <italic>L. garvieae</italic>. i.e the effect of bee venom on the resistance mechanism for <italic>L. garvieae</italic> was much less compared to <italic>V. anguillarum</italic>. Based on the results in the current study it could be concluded that applying bee venom to pathogenic bacteria that cause mortality in the aquaculture sector could induce the defense-related gene and change the broad-spectrum biocontrol activity at the molecular level.</p>
</abstract>

Bee venom (BV) is a rich source of secondary metabolites from honeybees (Apis mellifera L.). It contains a variety of bioactive ingredients including peptides, proteins, enzymes, and volatile metabolites. This study investigated the antibacterial effects of the bee venom obtained from honey bees (Apis mellifera L.) against bacterial fish pathogens, such as Lactococcus garvieae (Lg1, Lg2, Lg3), Vibrio anguillarum (Va1, Va2, Va3), Yersinia ruckeri (Yr1, Yr2, Yr3), and Aeromonas hydrophila (Ah1, Ah2, Ah3) and the expression levels on the antibiotic resistance genes hly and fbp (hemolysin and fibronectin-binding prot) of them. It was determined that bee venom had an antibacterial effect against L. garvieae, L. anguillarum, and Y. ruckeri strains, while it had no effect only against Ah3 and Ah2 bacterial strains. As staded by the gene expression of hly (hemolysin) and fbp (fibronectin-binding protein), among the antibiotic resistance genes the effect levels of bee venom on bacterial species varied, although it affected antibiotic resistance and gene expression level in all bacteria. It was revealed that the expression level was the highest for V. anguillarum strains, whereas it was below the control group for L. garvieae. i.e the effect of bee venom on the resistance mechanism for L. garvieae was much less compared to V. anguillarum. Based on the results in the current study it could be concluded that applying bee venom to pathogenic bacteria that cause mortality in the aquaculture sector could induce the defense-related gene and change the broad-spectrum biocontrol activity at the molecular level.

Determination of the antibacterial effect of bee venom against rainbow trout pathogens and antibiotic resistance gene expression

Muş Alparslan University, Faculty of Applied Sciences

Department of Animal Production and Technologies

Istanbul University, Faculty of Aquatic Sciences

Faculty of Fisheries, Department of Fish Diseases

Acta Veterinaria

This work is licensed under the Creative Commons Attribution 4.0 International License.

Bee venom (BV) is a rich source of secondary metabolites from honeybees (Apis mellifera L.). It contains a variety of bioactive ingredients including peptides,...

Determination of the antibacterial effect of bee venom against rainbow trout pathogens and antibiotic resistance gene expression

Online veröffentlicht: 28. Sept. 2023

Seitenbereich: 374 - 388

Eingereicht: 27. Apr. 2023

Akzeptiert: 07. Aug. 2023

DOI: https://doi.org/10.2478/acve-2023-0028

Schlüsselwörter
hly gen expression, Honeybee, Pathogenic fish bacteria

© 2023 Dilek Kabakci et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Determination of the antibacterial effect of bee venom against rainbow trout pathogens and antibiotic resistance gene expression

Online veröffentlicht: 28. Sept. 2023

Seitenbereich: 374 - 388

Eingereicht: 27. Apr. 2023

Akzeptiert: 07. Aug. 2023

DOI: https://doi.org/10.2478/acve-2023-0028

Schlüsselwörterhly gen expression, Honeybee, Pathogenic fish bacteria

© 2023 Dilek Kabakci et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Schlüsselwörter
hly gen expression, Honeybee, Pathogenic fish bacteria