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Apoptosis-related genes expression in primary in vitro culture of human ovarian granulosa cells

Ievgeniia Kocherova

Ievgeniia Kocherova

Department of Anatomy, Poznań University of Medical SciencesPoznań, Poland
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Kocherova, Ievgeniia
, 
Katarzyna Stefańska

Katarzyna Stefańska

Department of Histology and Embryology, Poznań University of Medical SciencesPoznań, Poland
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Stefańska, Katarzyna
, 
Rut Bryl

Rut Bryl

Department of Anatomy, Poznań University of Medical SciencesPoznań, Poland
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Bryl, Rut
, 
Joanna Perek

Joanna Perek

Department of Anatomy, Poznań University of Medical SciencesPoznań, Poland
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Perek, Joanna
, 
Wojciech Pieńkowski

Wojciech Pieńkowski

Division of Perinatology and Women’s Diseases, Poznań University of Medical SciencesPoznań, Poland
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Pieńkowski, Wojciech
, 
Jana Zakova

Jana Zakova

Department of Obstetrics and Gynecology, Faculty of Medicine, Masaryk University and University Hospital BrnoBrno, Czech Republic
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Zakova, Jana
, 
Igor Crha

Igor Crha

  • Department of Obstetrics and Gynecology, Faculty of Medicine, Masaryk University and University Hospital BrnoBrno, Czech Republic
  • Department of Nursing and Midwifery, Faculty of Medicine, Masaryk UniversityBrno, Czech Republic
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Crha, Igor
, 
Pavel Ventruba

Pavel Ventruba

Department of Obstetrics and Gynecology, Faculty of Medicine, Masaryk University and University Hospital BrnoBrno, Czech Republic
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Ventruba, Pavel
, 
Paul Mozdziak

Paul Mozdziak

Physiology Graduate Program, North Carolina State UniversityRaleigh, NC, USA
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Mozdziak, Paul
 und 
Michal Ješeta

Michal Ješeta

  • Department of Obstetrics and Gynecology, Faculty of Medicine, Masaryk University and University Hospital BrnoBrno, Czech Republic
  • Department of Veterinary Sciences, Czech University of Life Sciences in PraguePrague, Czech Republic
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Ješeta, Michal
  
31. Dez. 2020
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Online veröffentlicht: 31. Dez. 2020
Seitenbereich: 176 - 182
Eingereicht: 04. Nov. 2020
Akzeptiert: 15. Dez. 2020
DOI: https://doi.org/10.2478/acb-2020-0023
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© 2020 Ievgeniia Kocherova, Katarzyna Stefańska, Rut Bryl, Joanna Perek, Wojciech Pieńkowski, Jana Zakova, Igor Crha, Pavel Ventruba, Paul Mozdziak, Michal Ješeta, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1

BCL2 gene expression levels in GCs primary culture. Results are shown as mean ±SD. *p <0.05, ** p <0.01, *** p < 0.001
BCL2 gene expression levels in GCs primary culture. Results are shown as mean ±SD. *p <0.05, ** p <0.01, *** p < 0.001

Figure 2

BAX gene expression levels in GCs primary culture. Results are shown as mean ±SD. *p <0.05, ** p <0.01, *** p < 0.001
BAX gene expression levels in GCs primary culture. Results are shown as mean ±SD. *p <0.05, ** p <0.01, *** p < 0.001

Figure 3

P53 gene expression levels in GCs primary culture. Results are shown as mean ±SD. *p <0.05, ** p <0.01, *** p < 0.001
P53 gene expression levels in GCs primary culture. Results are shown as mean ±SD. *p <0.05, ** p <0.01, *** p < 0.001

Figure 4

CASP9 gene expression levels in GCs primary culture. Results are shown as mean ±SD. *p <0.05, ** p <0.01, *** p < 0.001
CASP9 gene expression levels in GCs primary culture. Results are shown as mean ±SD. *p <0.05, ** p <0.01, *** p < 0.001

Sequences of primers used in RT‐PCR for analysis of gene expression

GENEPRIMER SEQUENCE
BCL2F:ATGTGTGTGGAGAGCGTCAA
R:GAAATCAAACAGAGGCCGCA
BAXF:TGACATGTTTTCTGACGGCA
R:CACCCTGGTCTTGGATCCA
p53F:GCTGAATGAGGCCTTGGAAC
R:TTATGGCGGGAGGTAGACTG
CASP9F:TGATGTCGGTGCTCTTGAGA
R:CGCAACTTCTCACAGTCGAT
ACTBF:AAAGACCTGTACGCCAACAC
R:CTCAGGAGGAGCAATGATCTTG
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