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Apoptosis-related genes expression in primary in vitro culture of human ovarian granulosa cells

Ievgeniia Kocherova
Ievgeniia Kocherova
, 
Katarzyna Stefańska
Katarzyna Stefańska
, 
Rut Bryl
Rut Bryl
, 
Joanna Perek
Joanna Perek
, 
Wojciech Pieńkowski
Wojciech Pieńkowski
, 
Jana Zakova
Jana Zakova
, 
Igor Crha
Igor Crha
, 
Pavel Ventruba
Pavel Ventruba
, 
Paul Mozdziak
Paul Mozdziak
 und 
Michal Ješeta
Michal Ješeta
   | 31. Dez. 2020
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Online veröffentlicht: 31. Dez. 2020
Seitenbereich: 176 - 182
Eingereicht: 04. Nov. 2020
Akzeptiert: 15. Dez. 2020
DOI: https://doi.org/10.2478/acb-2020-0023
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© 2020 Ievgeniia Kocherova, Katarzyna Stefańska, Rut Bryl, Joanna Perek, Wojciech Pieńkowski, Jana Zakova, Igor Crha, Pavel Ventruba, Paul Mozdziak, Michal Ješeta, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1

BCL2 gene expression levels in GCs primary culture. Results are shown as mean ±SD. *p <0.05, ** p <0.01, *** p < 0.001
BCL2 gene expression levels in GCs primary culture. Results are shown as mean ±SD. *p <0.05, ** p <0.01, *** p < 0.001

Figure 2

BAX gene expression levels in GCs primary culture. Results are shown as mean ±SD. *p <0.05, ** p <0.01, *** p < 0.001
BAX gene expression levels in GCs primary culture. Results are shown as mean ±SD. *p <0.05, ** p <0.01, *** p < 0.001

Figure 3

P53 gene expression levels in GCs primary culture. Results are shown as mean ±SD. *p <0.05, ** p <0.01, *** p < 0.001
P53 gene expression levels in GCs primary culture. Results are shown as mean ±SD. *p <0.05, ** p <0.01, *** p < 0.001

Figure 4

CASP9 gene expression levels in GCs primary culture. Results are shown as mean ±SD. *p <0.05, ** p <0.01, *** p < 0.001
CASP9 gene expression levels in GCs primary culture. Results are shown as mean ±SD. *p <0.05, ** p <0.01, *** p < 0.001

Sequences of primers used in RT‐PCR for analysis of gene expression

GENEPRIMER SEQUENCE
BCL2F:ATGTGTGTGGAGAGCGTCAA
R:GAAATCAAACAGAGGCCGCA
BAXF:TGACATGTTTTCTGACGGCA
R:CACCCTGGTCTTGGATCCA
p53F:GCTGAATGAGGCCTTGGAAC
R:TTATGGCGGGAGGTAGACTG
CASP9F:TGATGTCGGTGCTCTTGAGA
R:CGCAACTTCTCACAGTCGAT
ACTBF:AAAGACCTGTACGCCAACAC
R:CTCAGGAGGAGCAATGATCTTG
eISSN:
2544-3577
Sprache:
Englisch
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4 Hefte pro Jahr
Fachgebiete der Zeitschrift:
Biologie, Molekularbiologie, Biochemie
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