<abstract xmlns="http://www.w3.org/1999/xhtml">The presence of CCs around the oocyte after ovulation is one of the key elements contributing to oocyte developmental competence. In the presented study, we used CCs from 12 patients aged 18-40 diagnosed with infertility. After harvesting cells on day 1, 7, 15 and 30 of culture, total RNA was isolated and transcriptomic analysis was performed. The DAVID software indicated the following GO BP terms: “cell junction organization”, “cell migration”, “cell morphogenesis involved in differentiation”, “cell morphogenesis” and “cell motility”. Of the genes belonging to all ontological groups, the most downregulated were: SLC7A8, DFNB31, COL1A1, CDC42SE1, TGFBR3, HMGB1, with the most upregulated genes being: ANXA3, KIAA1199, HTR2B, VCAM1, DKK1.While many studies focus on attempts to obtain fully competent oocytes, scientists still have difficulty attaining adequate results in vitro. Lack of adequate knowledge often results in low in vitro fertilization efficiency. Therefore, our research focuses on CCs cells, thanks to which the oocyte most likely acquires developmental competence. The main purpose of the study was to identify the potential molecular markers responsible for cell junction organization, migration, differentiation, morphogenesis and motility.<bold>Running title</bold>: New markers of human cumulus oophorus cells cultured <italic>in vitro</italic></abstract>

The presence of CCs around the oocyte after ovulation is one of the key elements contributing to oocyte developmental competence. In the presented study, we used CCs from 12 patients aged 18-40 diagnosed with infertility. After harvesting cells on day 1, 7, 15 and 30 of culture, total RNA was isolated and transcriptomic analysis was performed. The DAVID software indicated the following GO BP terms: “cell junction organization”, “cell migration”, “cell morphogenesis involved in differentiation”, “cell morphogenesis” and “cell motility”. Of the genes belonging to all ontological groups, the most downregulated were: SLC7A8, DFNB31, COL1A1, CDC42SE1, TGFBR3, HMGB1, with the most upregulated genes being: ANXA3, KIAA1199, HTR2B, VCAM1, DKK1.While many studies focus on attempts to obtain fully competent oocytes, scientists still have difficulty attaining adequate results in vitro. Lack of adequate knowledge often results in low in vitro fertilization efficiency. Therefore, our research focuses on CCs cells, thanks to which the oocyte most likely acquires developmental competence. The main purpose of the study was to identify the potential molecular markers responsible for cell junction organization, migration, differentiation, morphogenesis and motility.Running title: New markers of human cumulus oophorus cells cultured in vitro

New markers of human cumulus oophorus cells cultured in vitro – transcriptomic profile

Medical Journal of Cell Biology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The presence of CCs around the oocyte after ovulation is one of the key elements contributing to oocyte developmental competence. In the presented study, we...

New markers of human cumulus oophorus cells cultured in vitro – transcriptomic profile

Online veröffentlicht: 29. Apr. 2020

Seitenbereich: 60 - 72

Eingereicht: 25. Feb. 2020

Akzeptiert: 03. Apr. 2020

DOI: https://doi.org/10.2478/acb-2020-0007

Schlüsselwörter
cumulus ophorus cells, microarrays, human reproduction

© 2020 Maciej Brązert, Wiesława Kranc, Karol Jopek, Bartosz Kempisty, Leszek Pawelczyk, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

New markers of human cumulus oophorus cells cultured in vitro – transcriptomic profile

Online veröffentlicht: 29. Apr. 2020

Seitenbereich: 60 - 72

Eingereicht: 25. Feb. 2020

Akzeptiert: 03. Apr. 2020

DOI: https://doi.org/10.2478/acb-2020-0007

Schlüsselwörtercumulus ophorus cells, microarrays, human reproduction

© 2020 Maciej Brązert, Wiesława Kranc, Karol Jopek, Bartosz Kempisty, Leszek Pawelczyk, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Schlüsselwörter
cumulus ophorus cells, microarrays, human reproduction