Evaluation of the radiosensitizing effect of MEK inhibitor KZ-001 on non-small cell lung cancer cells in vitro
Artikel-Kategorie: Original article
Online veröffentlicht: 26. Okt. 2023
Seitenbereich: 230 - 237
DOI: https://doi.org/10.2478/abm-2023-0064
Schlüsselwörter
© 2023 Gongchao Huang et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.
Background
Non-small cell lung cancer (NSCLC) has a poor prognosis and usually presents resistance against radiotherapy. MEK inhibitors have been proven to possess a radiosensitization effect. The compound KZ-001 as a particular MEK inhibitor is superior to the listed MEK inhibitor AZD6244.
Objective
To investigate whether KZ-001 could enhance the radiosensitivity of NSCLC cell lines in vitro.
Methods
MTT and colony formation assay were used to evaluate the radiosensitivity effect of KZ-001. Immunofluorescence, cell cycle, apoptosis staining, and western blot experiments were used to explore the radiosensitivity mechanism.
Results
KZ-001 significantly decreased A549 cell viability at 6 Gy and 8 Gy radiation doses and caused the radiosensitivity at 1 Gy, 4 Gy, and 6 Gy in colony formation experiments. The A549 apoptosis ratio induced by irradiation (IR) combined with KZ-001 increased significantly in comparison with that by IR monotherapy (10.57% vs. 6.23%,
Conclusions
MEK1/2 inhibitor KZ-001 is a potential radiosensitizer for clinical applications.