<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml">The micronucleus assay in human peripheral blood lymphocytes is a sensitive indicator of radiation damage and could serve as a biological dosimeter in evaluating suspected overexposure to ionising radiation. Micronucleus (MN) frequency as a measure of chromosomal damage has also extensively been employed to quantify the effects of radiation dose rate on biological systems. Here we studied the effects of 8 MeV pulsed electron beam emitted by Microtron electron accelerator on MN induction at dose rates between 35 Gy min-1 and 352.5 Gy min-1. These dose rates were achieved by varying the pulse repetition rate (PRR). Fricke dosimeter was employed to measure the absorbed dose at different PRR and to ensure uniform dose distribution of the electron beam. To study the dose rate effect, blood samples were irradiated to an absorbed dose of (4.7±0.2) Gy at different rates and cytogenetic damage was quantified using the micronucleus assay. The obtained MN frequency showed no dose rate dependence within the studied dose rate range. Our earlier dose effect study using 8 MeV electrons revealed that the response of MN was linear-quadratic. Therefore, in the event of an accident, dose estimation can be made using linear-quadratic dose response parameters, without adding dose rate as a correction factor.</abstract>

The micronucleus assay in human peripheral blood lymphocytes is a sensitive indicator of radiation damage and could serve as a biological dosimeter in evaluating suspected overexposure to ionising radiation. Micronucleus (MN) frequency as a measure of chromosomal damage has also extensively been employed to quantify the effects of radiation dose rate on biological systems. Here we studied the effects of 8 MeV pulsed electron beam emitted by Microtron electron accelerator on MN induction at dose rates between 35 Gy min-1 and 352.5 Gy min-1. These dose rates were achieved by varying the pulse repetition rate (PRR). Fricke dosimeter was employed to measure the absorbed dose at different PRR and to ensure uniform dose distribution of the electron beam. To study the dose rate effect, blood samples were irradiated to an absorbed dose of (4.7±0.2) Gy at different rates and cytogenetic damage was quantified using the micronucleus assay. The obtained MN frequency showed no dose rate dependence within the studied dose rate range. Our earlier dose effect study using 8 MeV electrons revealed that the response of MN was linear-quadratic. Therefore, in the event of an accident, dose estimation can be made using linear-quadratic dose response parameters, without adding dose rate as a correction factor.

Dose Rate Effect of Pulsed Electron Beam on Micronucleus Frequency in Human Peripheral Blood Lymphocytes

Microtron Centre, Department of Studies in Physics, Mangalore University, Mangalagangotri, India

Radiological Physics and Advisory Division, Bhabha Atomic Research Centre, Mumbai, India

Archives of Industrial Hygiene and Toxicology

The micronucleus assay in human peripheral blood lymphocytes is a sensitive indicator of radiation damage and could serve as a biological dosimeter in...

Dose Rate Effect of Pulsed Electron Beam on Micronucleus Frequency in Human Peripheral Blood Lymphocytes

Online veröffentlicht: 25. März 2010

Seitenbereich: 77 - 83

DOI: https://doi.org/10.2478/10004-1254-61-2010-1982

Schlüsselwörter
akcelerator, dozimetrija, elektronski snop, PRR

This content is open access.

Dose Rate Effect of Pulsed Electron Beam on Micronucleus Frequency in Human Peripheral Blood Lymphocytes

Online veröffentlicht: 25. März 2010

Seitenbereich: 77 - 83

DOI: https://doi.org/10.2478/10004-1254-61-2010-1982

Schlüsselwörterakcelerator, dozimetrija, elektronski snop, PRR

This content is open access.

Schlüsselwörter
akcelerator, dozimetrija, elektronski snop, PRR