<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml">Irinotecan is a topoisomerase I interactive agent, widely used in the treatment of metastatic colorectal cancer. The genotoxic effects of the maximum single dose (18 μg mL-1), recommended monotherapy dose (9 μg mL-1), and recommended combined therapy dose (4.5 μg mL-1) of irinotecan were studied on V79 cells using the comet assay, chromosome aberration assay, and micronucleus test. The cells were treated with irinotecan for 2 h or 24 h. The statistical significance of the results was determined using the one-way ANOVA test and a nonparametric Mann Whitney U test. The comet assay did not show dose-dependent or time-dependent effects. The chromosome aberration analysis showed large DNA rearrangements, i.e., chromosome exchanges. Although the exposed cultures showed a significant increase in micronucleated cells in respect to control, no dose-dependent relation was established among the treated cultures. Time-dependent effect was also not observed.</abstract>

Irinotecan is a topoisomerase I interactive agent, widely used in the treatment of metastatic colorectal cancer. The genotoxic effects of the maximum single dose (18 μg mL-1), recommended monotherapy dose (9 μg mL-1), and recommended combined therapy dose (4.5 μg mL-1) of irinotecan were studied on V79 cells using the comet assay, chromosome aberration assay, and micronucleus test. The cells were treated with irinotecan for 2 h or 24 h. The statistical significance of the results was determined using the one-way ANOVA test and a nonparametric Mann Whitney U test. The comet assay did not show dose-dependent or time-dependent effects. The chromosome aberration analysis showed large DNA rearrangements, i.e., chromosome exchanges. Although the exposed cultures showed a significant increase in micronucleated cells in respect to control, no dose-dependent relation was established among the treated cultures. Time-dependent effect was also not observed.

Assessment of Cyto/Genotoxicity of Irinotecan in V79 Cells Using the Comet, Micronucleus, and Chromosome Aberration Assay

Institute for Medical Research and Occupational Health, Zagreb, Croatia

Clinical Hospital Centre Zagreb, Oncology Clinic, Zagreb, Croatia

Archives of Industrial Hygiene and Toxicology

Irinotecan is a topoisomerase I interactive agent, widely used in the treatment of metastatic colorectal cancer. The genotoxic effects of the maximum single...

Assessment of Cyto/Genotoxicity of Irinotecan in V79 Cells Using the Comet, Micronucleus, and Chromosome Aberration Assay

Online veröffentlicht: 25. März 2010

Seitenbereich: 1 - 9

DOI: https://doi.org/10.2478/10004-1254-61-2010-1967

Schlüsselwörter
antineoplastični lijekovi, citogenetska oštećenja, oštećenje DNA, stanične kulture, topoizomeraze

This content is open access.

Assessment of Cyto/Genotoxicity of Irinotecan in V79 Cells Using the Comet, Micronucleus, and Chromosome Aberration Assay

Online veröffentlicht: 25. März 2010

Seitenbereich: 1 - 9

DOI: https://doi.org/10.2478/10004-1254-61-2010-1967

Schlüsselwörterantineoplastični lijekovi, citogenetska oštećenja, oštećenje DNA, stanične kulture, topoizomeraze

This content is open access.

Schlüsselwörter
antineoplastični lijekovi, citogenetska oštećenja, oštećenje DNA, stanične kulture, topoizomeraze