<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The aim of this study was to determine if there are quantitative differences in <italic>Candida</italic> fungi between pediatric patients with Crohn’s disease (before and after exclusive enteral nutrition (EEN), and the biologic therapy with anti-tumor necrosis factor alpha – (IFX)), and healthy controls. DNA was isolated from fecal samples and PCR was used to determine the number of fungal cells. Both therapeutic interventions resulted in a statistically significant decrease in Pediatric Crohn’s Disease Activity Index. The numbers of <italic>Candida</italic> decreased during both therapeutic intervention but the difference was statistically significant for the IFX intervention only (<italic>p</italic> = 0.045). Moreover, fungi population in both study groups declined during intervention when compared to the control group but the difference was significant before treatment only in the IFX group (<italic>p</italic> = 0.013). The total distribution of <italic>Candida</italic> with both IFX and EEN as well as in the control group differed significantly (<italic>p</italic> = 0.01) before treatment only. No correlation between the numbers of <italic>Candida</italic> and disease activity as well as the following biochemical parameters: serum iron concentration, protein or glucose level were found. It cannot be ruled out that, in combination with genetic and immunological disorders, fungi can contribute to the initiation of the disease process and perpetuation of active inflammation.</p></abstract>

The aim of this study was to determine if there are quantitative differences in Candida fungi between pediatric patients with Crohn’s disease (before and after exclusive enteral nutrition (EEN), and the biologic therapy with anti-tumor necrosis factor alpha – (IFX)), and healthy controls. DNA was isolated from fecal samples and PCR was used to determine the number of fungal cells. Both therapeutic interventions resulted in a statistically significant decrease in Pediatric Crohn’s Disease Activity Index. The numbers of Candida decreased during both therapeutic intervention but the difference was statistically significant for the IFX intervention only (p = 0.045). Moreover, fungi population in both study groups declined during intervention when compared to the control group but the difference was significant before treatment only in the IFX group (p = 0.013). The total distribution of Candida with both IFX and EEN as well as in the control group differed significantly (p = 0.01) before treatment only. No correlation between the numbers of Candida and disease activity as well as the following biochemical parameters: serum iron concentration, protein or glucose level were found. It cannot be ruled out that, in combination with genetic and immunological disorders, fungi can contribute to the initiation of the disease process and perpetuation of active inflammation.

Dependence of Colonization of the Large Intestine by <italic>Candida</italic> on the Treatment of Crohn’s Disease

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Dependence of Colonization of the Large Intestine by  on the Treatment of Crohn’s Disease

The aim of this study was to determine if there are quantitative differences in Candida fungi between pediatric patients with Crohn’s disease (before and after...

Characteristics	Biologic therapy – IFX (n = 13)	EEN (n = 48)	Control group (n = 17)
Male:Female, n (%)	7 (54%):6 (46%)	29 (60%):19 (40%)	9 (53%):8 (47%)
Age at diagnosis, months; mean (± SD)	137 (± 48.15)	160.27 (± 37.11)	N/A
Age at initial treatment, months; mean (± SD)	157.15 (± 45.16)	160.27 (± 37.11)	N/A
Weight, kg; mean (± SD)	41.97 (± 16.3)	40.93 (± 14.05)	42.8 (± 17.2)
Height, cm; mean (± SD)	149.95 (± 20.31)	155.3 (± 19.1)	148.7 (± 18.8)
BMI, kg/m²; mean (± SD)	17.89 (± 3.62)	16.4 (± 2.92)	18.3 (± 3.8)
PCDAI-1; mean (± SD)	47.5 (± 16.43)	32.03 (± 15.01)	N/A
PCDAI-2; mean (± SD)	9.04 (± 6.5)	5.93 (± 11.36)	N/A

Dependence of Colonization of the Large Intestine by Candida on the Treatment of Crohn’s Disease

Article Category: original-paper

Online veröffentlicht: 28. März 2019

Seitenbereich: 121 - 126

DOI: https://doi.org/10.21307/pjm-2019-014

Schlüsselwörter
Crohn’s disease, children, gut microbiota, biological treatment

© 2019 Kinga Kowalska-Duplaga et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Fig. 2.

Baseline patient characteristics.

Dependence of Colonization of the Large Intestine by Candida on the Treatment of Crohn’s Disease

Article Category: original-paper

Online veröffentlicht: 28. März 2019

Seitenbereich: 121 - 126

DOI: https://doi.org/10.21307/pjm-2019-014

SchlüsselwörterCrohn’s disease, children, gut microbiota, biological treatment

© 2019 Kinga Kowalska-Duplaga et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Fig. 2.

Baseline patient characteristics.

Schlüsselwörter
Crohn’s disease, children, gut microbiota, biological treatment