<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Few publications report the occurrence of bacteria associated with fungal cells. The presence of bacteria associated with one strain of <italic>Pleurotus ostreatus</italic> (Fr.) P. Kumm. was described in the literature. We describe the biodiversity of bacteria associated with eight oyster mushroom strains from Japan, Poland, and the USA. The presence of microorganisms associated with all tested <italic>P. ostreatus</italic> strains was confirmed using fluorescent microscopy. Among 307 sequences, 233 of clones representing 34 genera and 74 sequences were identified as <italic>Bacteria</italic>. Most of the bacteria associated with the strain PUSAS were related to <italic>E. coli</italic> and two clones were related to <italic>Cupriavidus</italic> genus. The biodiversity of clones isolated from fungal strains originating from Japan and Poland ranged from 15 to 32 different bacterial clones. The most often the bacteria related to genus <italic>Curvibacter, Pseudomonas, Bacillus, Cupriavidus, Pelomonas</italic>, and <italic>Propionibacterium</italic> were associated with the strains of fungi mentioned above. Laccase-like (LMCO) genes were identified in whole bacterial DNA isolated from the associated bacteria but β-glucosidase and β-xylanase genes were not detected.</p></abstract>

Few publications report the occurrence of bacteria associated with fungal cells. The presence of bacteria associated with one strain of Pleurotus ostreatus (Fr.) P. Kumm. was described in the literature. We describe the biodiversity of bacteria associated with eight oyster mushroom strains from Japan, Poland, and the USA. The presence of microorganisms associated with all tested P. ostreatus strains was confirmed using fluorescent microscopy. Among 307 sequences, 233 of clones representing 34 genera and 74 sequences were identified as Bacteria. Most of the bacteria associated with the strain PUSAS were related to E. coli and two clones were related to Cupriavidus genus. The biodiversity of clones isolated from fungal strains originating from Japan and Poland ranged from 15 to 32 different bacterial clones. The most often the bacteria related to genus Curvibacter, Pseudomonas, Bacillus, Cupriavidus, Pelomonas, and Propionibacterium were associated with the strains of fungi mentioned above. Laccase-like (LMCO) genes were identified in whole bacterial DNA isolated from the associated bacteria but β-glucosidase and β-xylanase genes were not detected.

Biodiversity of Bacteria Associated with Eight <italic>Pleurotus ostreatus</italic> (Fr.) P. Kumm. Strains from Poland, Japan and the USA

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Biodiversity of Bacteria Associated with Eight  (Fr.) P. Kumm. Strains from Poland, Japan and the USA

Few publications report the occurrence of bacteria associated with fungal cells. The presence of bacteria associated with one strain of Pleurotus ostreatus...

Biodiversity of Bacteria Associated with Eight Pleurotus ostreatus (Fr.) P. Kumm. Strains from Poland, Japan and the USA

Article Category: original-paper

Online veröffentlicht: 28. März 2019

Seitenbereich: 71 - 81

DOI: https://doi.org/10.21307/pjm-2019-009

Schlüsselwörter
biodiversity, associated bacteria, fluorescent microscopy, 16S rRNA PCR

© 2019 Mariusz Adamski and Stanisław J. Pietr et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Biodiversity of Bacteria Associated with Eight Pleurotus ostreatus (Fr.) P. Kumm. Strains from Poland, Japan and the USA

Article Category: original-paper

Online veröffentlicht: 28. März 2019

Seitenbereich: 71 - 81

DOI: https://doi.org/10.21307/pjm-2019-009

Schlüsselwörterbiodiversity, associated bacteria, fluorescent microscopy, 16S rRNA PCR

© 2019 Mariusz Adamski and Stanisław J. Pietr et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Schlüsselwörter
biodiversity, associated bacteria, fluorescent microscopy, 16S rRNA PCR