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Bioactive Compounds of Pseudoalteromonas sp. IBRL PD4.8 Inhibit Growth of Fouling Bacteria and Attenuate Biofilms of Vibrio alginolyticus FB3

NOR AFIFAH SUPARDY
NOR AFIFAH SUPARDY
, 
DARAH IBRAHIM
DARAH IBRAHIM
, 
SHARIFAH RADZIAH MAT NOR
SHARIFAH RADZIAH MAT NOR
 und 
WAN NORHANA MD NOORDIN
WAN NORHANA MD NOORDIN
   | 27. März 2019
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Article Category: original-paper
Online veröffentlicht: 27. März 2019
Seitenbereich: 21 - 33
DOI: https://doi.org/10.21307/pjm-2019-003
Schlüsselwörter
© 2019 Maryam Labibzadeh et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Phylogenetic tree of Pseudoalteromonas sp. IBRL PD4.8 isolated from the surface of C. racemosa.
Phylogenetic tree of Pseudoalteromonas sp. IBRL PD4.8 isolated from the surface of C. racemosa.

Fig. 2.

Agar-overlay assay of ethyl acetate extract of Pseudoalteromonas sp. IBRL PD4.8 against V. alginolyticus FB3.Circle (1) light yellow, Rf 0.06; (2) fluorescent blue, Rf 0.35; (3) fluorescent green, Rf 0.55.
Agar-overlay assay of ethyl acetate extract of Pseudoalteromonas sp. IBRL PD4.8 against V. alginolyticus FB3.Circle (1) light yellow, Rf 0.06; (2) fluorescent blue, Rf 0.35; (3) fluorescent green, Rf 0.55.

Fig. 3.

Antibiofilm activity of Pseudoalteromonas sp. IBRL PD4.8 extract against initial and pre-formed biofilm of V. alginolyticus FB3. Data of inhibition percentages are presented in the table below the figure.
Antibiofilm activity of Pseudoalteromonas sp. IBRL PD4.8 extract against initial and pre-formed biofilm of V. alginolyticus FB3. Data of inhibition percentages are presented in the table below the figure.

Fig. 4.

The light microscopy images of V. alginolyticus FB3 biofilms treated with Pseudoalteromonas sp. IBRL PD4.8 extract.A. Untreated initial biofilm; B. initial biofilm treated with 8.0 mg/ml; C. untreated pre-formed biofilm; D. pre-formed biofilm treated with 8.0 mg/ml; bar = 20 µm.
The light microscopy images of V. alginolyticus FB3 biofilms treated with Pseudoalteromonas sp. IBRL PD4.8 extract.A. Untreated initial biofilm; B. initial biofilm treated with 8.0 mg/ml; C. untreated pre-formed biofilm; D. pre-formed biofilm treated with 8.0 mg/ml; bar = 20 µm.

Fig. 5.

The scanning electron microscopic images of V. alginolyticus FB3 biofilms treated with the extract at a concentration of 8.0 mg/ml.A. untreated initial biofilm (10 000×); B. treated initial biofilm (10 000×); C. untreated pre-formed biofilm (5000×); D. treated pre-formed biofilm (5000×).
The scanning electron microscopic images of V. alginolyticus FB3 biofilms treated with the extract at a concentration of 8.0 mg/ml.A. untreated initial biofilm (10 000×); B. treated initial biofilm (10 000×); C. untreated pre-formed biofilm (5000×); D. treated pre-formed biofilm (5000×).

MIC values for crude extract and fraction F3 against fouling bacteria.

Fouling bacteriaCrude (mg/ml), MICF3 (mg/ml), MIC
Vibrio alginolyticus FB38.008.00
Pseuodoaltermonas sp. FB48.004.00
Alteromonas sp. FB78.008.00
Pseudoalteromonas sp. FB98.008.00
Bacillus sp. FB130.130.50

Characteristics of compounds from LC-MS analysis of sub-fraction F3a.

No.RT (min)CompoundMassm/z [M-H]−FormulaScore (DB)
14.233Sulfate97.96796.9597H2O4S98.79
24.9064,7,10,13-hexadecatetraenoic acid248.1763293.1748C16H24O284.93
35.662Homoveratric acid196.0729241.071C10H12O495.41
46.809Isoacitretin326.1891325.1821C21H26O384.11
56.923Sodium tetradecyl sulfate294.1859293.1779C14H30O4S86.66
67.617D1-2-Hydroxymethylethisteron340.2048339.1978C22H8O385.00

Details of BLAST result, % similarity, and accession numbers of all isolates.

IsolateSequence length (bp)BLAST result% SimilarityAccession No.
PD4.81385Pseudoalteromonas shiyosakiensis99LC131142.1
FB31349Vibrio alginolyticus97KC884661.1
FB41256Pseudoalteromonas sp.96JX0705058.1
FB71439Alteromonas sp.93EF061415.1
FB91398Pseudoalteromonas sp.94JX075059.1
FB131446Bacillus sp.93DQ448746.1
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