<abstract xmlns="http://www.w3.org/1999/xhtml"><p>A collection of 206 <italic>Staphylococcus</italic> spp. isolates was investigated for their ability to produce compounds exhibiting antistaphylococcal activity. This group included <italic>Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus xylosus</italic> strains recovered from bovine mastitis (n = 158) and human skin wounds and soft tissues infections (n = 48). Production of substances with antimicrobial activity was observed in six strains. Five of them were recovered from bovine mastitis, and one was isolated from the infected human skin wound. Three of the six antimicrobials produced by the different strains showed substantial loss of antimicrobial activity upon treatment with proteolytic enzymes, which suggests their peptidic structure. Additional studies have shown that one of the putative bacteriocins was efficiently secreted to the liquid medium, facilitating its large-scale production and isolation. The peptide produced by the M2B strain exhibited promising activity; however, against narrow spectrum of <italic>Staphylococcus</italic> spp. clinical and animal isolates. Growth inhibition was observed only in the case of 13 (including nine <italic>S. aureus</italic>, three <italic>S. xylosus</italic> and one <italic>S. epidermidis</italic> strains) out of 206 strains tested. Important advantage of the produced agent was its high thermal stability. Fifteen minutes of incubation at 90°C did not affect its antimicrobial potential. The highest efficiency of production of the agent was demonstrated in TSB medium after 24 hours at 37°C. The researches revealed that ability to production of bacteriocin among staphylococci is not very common. Only one (<italic>S. xylosus</italic> strain assigned as M2B) out of 206 strains tested produced satisfactory amounts of antistaphylococcal bacteriocin. In spite of that, we would encourage other researchers for investigation of their collections of <italic>Staphylococcus</italic> spp. isolates towards selection strains producing antimicrobial agents.</p></abstract>

A collection of 206 Staphylococcus spp. isolates was investigated for their ability to produce compounds exhibiting antistaphylococcal activity. This group included Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus xylosus strains recovered from bovine mastitis (n = 158) and human skin wounds and soft tissues infections (n = 48). Production of substances with antimicrobial activity was observed in six strains. Five of them were recovered from bovine mastitis, and one was isolated from the infected human skin wound. Three of the six antimicrobials produced by the different strains showed substantial loss of antimicrobial activity upon treatment with proteolytic enzymes, which suggests their peptidic structure. Additional studies have shown that one of the putative bacteriocins was efficiently secreted to the liquid medium, facilitating its large-scale production and isolation. The peptide produced by the M2B strain exhibited promising activity; however, against narrow spectrum of Staphylococcus spp. clinical and animal isolates. Growth inhibition was observed only in the case of 13 (including nine S. aureus, three S. xylosus and one S. epidermidis strains) out of 206 strains tested. Important advantage of the produced agent was its high thermal stability. Fifteen minutes of incubation at 90°C did not affect its antimicrobial potential. The highest efficiency of production of the agent was demonstrated in TSB medium after 24 hours at 37°C. The researches revealed that ability to production of bacteriocin among staphylococci is not very common. Only one (S. xylosus strain assigned as M2B) out of 206 strains tested produced satisfactory amounts of antistaphylococcal bacteriocin. In spite of that, we would encourage other researchers for investigation of their collections of Staphylococcus spp. isolates towards selection strains producing antimicrobial agents.

Isolation of Bacteriocin-producing <italic>Staphylococcus</italic> spp. Strains from Human Skin Wounds, Soft Tissue Infections and Bovine Mastitis

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Isolation of Bacteriocin-producing  spp. Strains from Human Skin Wounds, Soft Tissue Infections and Bovine Mastitis

A collection of 206 Staphylococcus spp. isolates was investigated for their ability to produce compounds exhibiting antistaphylococcal activity. This group...

Strain	Source of the strain	Resistance
1 S. xylosus 30c1	Bovine mastitis	ND
2 S. epidermidis 267	Bovine mastitis	ND
3 S. aureus K10	Human skin wound (Koszalin)	Susceptible
4 S. aureus 11	Bovine mastitis	P, AMP, AML, S, AMC^*
5 S. aureus 27	Bovine mastitis	N, MY, S, E, DA^*
6 S. aureus 101 (MRSA)	Bovine mastitis	P+DA+E+MY+S+N/OX^*
7 L. monocytogenes ATCC10425	Reference strain	ND

No. of strain	Code number of the sensitive strain	Growth inhibition zone [mm]
1	7 (S. aureus)	12
2	11 (S. aureus)	20
3	28 (S. aureus)	15
4	52 (S. aureus)	22
5	68 (S. aureus)	25
6	77 (S. aureus)	18
7	92 (S. aureus)	17
8	99 (S. aureus)	25
9	115 (S. aureus)	15
10	83A (S. epidermidis)	15
11	8J (S. xylosus)	15
12	30C1 (S. xylosus)	15
13	247 (S. xylosus)	15
14	L. monocytogenes	15
15	M. luteus	15

Isolation of Bacteriocin-producing Staphylococcus spp. Strains from Human Skin Wounds, Soft Tissue Infections and Bovine Mastitis

Article Category: original-paper

Online veröffentlicht: 30. Juni 2018

Seitenbereich: 163 - 170

Eingereicht: 08. Aug. 2017

Akzeptiert: 20. Nov. 2017

DOI: https://doi.org/10.21307/pjm-2018-018

Schlüsselwörter
antimicrobial peptides, antistaphylococcal agents,

© 2018 Magdalena Zalewska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Fig. 2.

Fig. 3.

The indicator strains used for preliminary selections of staphylococci producing antibacterial agents.

Activity of newly identified agent against strains from the Departments’ collection.

Isolation of Bacteriocin-producing Staphylococcus spp. Strains from Human Skin Wounds, Soft Tissue Infections and Bovine Mastitis

Article Category: original-paper

Online veröffentlicht: 30. Juni 2018

Seitenbereich: 163 - 170

Eingereicht: 08. Aug. 2017

Akzeptiert: 20. Nov. 2017

DOI: https://doi.org/10.21307/pjm-2018-018

Schlüsselwörterantimicrobial peptides, antistaphylococcal agents,

© 2018 Magdalena Zalewska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Fig. 2.

Fig. 3.

The indicator strains used for preliminary selections of staphylococci producing antibacterial agents.

Activity of newly identified agent against strains from the Departments’ collection.

Schlüsselwörter
antimicrobial peptides, antistaphylococcal agents,