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Pristionchus trametes n. sp. (Diplogastridae) isolated from the mushroom Trametes orientalis in Kyoto, Japan

Natsumi Kanzaki
Natsumi Kanzaki
 und 
Keiko Hamaguchi
Keiko Hamaguchi
   | 19. Juli 2021
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Online veröffentlicht: 19. Juli 2021
Seitenbereich: 1 - 13
DOI: https://doi.org/10.21307/jofnem-2021-060
Schlüsselwörter
© 2021 Natsumi Kanzaki et al., published by Sciendo.
This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1:

Pristionchus trametes n. sp. adults in the right lateral view. A: Female (ov, ovary; jt, junction tissue; sp, spermatheca; od, oviduct; ut, uterus; v, vulva; pd, postdeirid). B: Male (ref, reflexed part of testis; tes, testis; vd, vas deferens).
Pristionchus trametes n. sp. adults in the right lateral view. A: Female (ov, ovary; jt, junction tissue; sp, spermatheca; od, oviduct; ut, uterus; v, vulva; pd, postdeirid). B: Male (ref, reflexed part of testis; tes, testis; vd, vas deferens).

Figure 2:

Pristionchus trametes n. sp. adults. A: Surface of the lip region. B, C: Stomatal region. D: Anterior part of an adult. E: Vulval region. F, G: Female tail (ph, phasmid). H: Male tail (v + number, ad, genital papillae where “d” indicates laterally oriented papillae; ph, phasmid). I: Male tail tip (labels are same as H). J: Spicule and gubernaculum. A and B, left lateral view; E and G, ventral view; the others, right lateral view.
Pristionchus trametes n. sp. adults. A: Surface of the lip region. B, C: Stomatal region. D: Anterior part of an adult. E: Vulval region. F, G: Female tail (ph, phasmid). H: Male tail (v + number, ad, genital papillae where “d” indicates laterally oriented papillae; ph, phasmid). I: Male tail tip (labels are same as H). J: Spicule and gubernaculum. A and B, left lateral view; E and G, ventral view; the others, right lateral view.

Figure 3:

Pristionchus trametes n. sp. adults.A: Right lateral view of the stoma-pharyngeal region in four different focal planes (ep, secretory-excretory pore; car, cardia; nr, nerve ring; dr, deirid). B: Left lateral view of the stomatal region in three different focal planes (rsv, right subventral tooth; d, dorsal tooth; lsv, left subventral denticles).
Pristionchus trametes n. sp. adults.A: Right lateral view of the stoma-pharyngeal region in four different focal planes (ep, secretory-excretory pore; car, cardia; nr, nerve ring; dr, deirid). B: Left lateral view of the stomatal region in three different focal planes (rsv, right subventral tooth; d, dorsal tooth; lsv, left subventral denticles).

Figure 4:

Pristionchus trametes n. sp. adults. A: Left lateral view of the entire male tail (v + number, ad, genital papillae where “d” indicates laterally oriented papillae; co, cloacal opening). B: Spicule and gubernaculum in left lateral view. C: Left lateral view of the distal part of the male tail in two different focal planes (v + number, pd, genital papillae; ph, phasmid). D: Right lateral view of the female tail in two different focal planes (an, anus; ph, phasmid; rec, rectum). E: Ventral view of the female tail in four different focal planes (an, anus; ph, phasmid; rg, rectal glands).
Pristionchus trametes n. sp. adults. A: Left lateral view of the entire male tail (v + number, ad, genital papillae where “d” indicates laterally oriented papillae; co, cloacal opening). B: Spicule and gubernaculum in left lateral view. C: Left lateral view of the distal part of the male tail in two different focal planes (v + number, pd, genital papillae; ph, phasmid). D: Right lateral view of the female tail in two different focal planes (an, anus; ph, phasmid; rec, rectum). E: Ventral view of the female tail in four different focal planes (an, anus; ph, phasmid; rg, rectal glands).

Figure 5:

Phylogenetic relationships of Pristionchus spp. A Bayesian tree was inferred from concatenated SSU and D1-D4 LSU sequences under the GTR + G + I model. The following analytical parameters were used: SSU, freqA = 0.25, freqC = 0.21, freqG = 0.26, freqT = 0.28, R(a) = 0.77, R(b) = 2.52, R(c) = 2.09, R(d) = 0.83, R(e) = 3.32, R(f) = 1.00, Pinva = 0.46, Shape = 0.48; D1-D4 LSU, freqA = 0.23, freqC = 0.22, freqG = 0.31, freqT = 0.25, R(a) = 0.57, R(b) = 2.43, R(c) = 0.94, R(d) = 0.68, R(e) = 4.39, R(f) = 1.00, Pinva = 0.39, Shape = 0.49. Posterior probability values exceeding 50% are shown for each clade.
Phylogenetic relationships of Pristionchus spp. A Bayesian tree was inferred from concatenated SSU and D1-D4 LSU sequences under the GTR + G + I model. The following analytical parameters were used: SSU, freqA = 0.25, freqC = 0.21, freqG = 0.26, freqT = 0.28, R(a) = 0.77, R(b) = 2.52, R(c) = 2.09, R(d) = 0.83, R(e) = 3.32, R(f) = 1.00, Pinva = 0.46, Shape = 0.48; D1-D4 LSU, freqA = 0.23, freqC = 0.22, freqG = 0.31, freqT = 0.25, R(a) = 0.57, R(b) = 2.43, R(c) = 0.94, R(d) = 0.68, R(e) = 4.39, R(f) = 1.00, Pinva = 0.39, Shape = 0.49. Posterior probability values exceeding 50% are shown for each clade.

GenBank accession numbers for the sequences used for the phylogenetic analysis.

Species SSU LSU
Parapristionchus giblindavisi JX163981 JX163972
Pristionchus aerivorus FJ040440 KT188862
Pristionchus americanus FJ040445 KT188867
Pristionchus arcanus KT188848 KT188878
Pristionchus atlanticus KT188839 KT188869
Pristionchus boliviae KT188838 KT188868
Pristionchus borbonicus KT188856 KT188885
Pristionchus brevicauda KT188841 KT188871
Pristionchus bucculentus KT188860 KT188889
Pristionchus bulgaricus KT188845 KT188875
Pristionchus clavus KT188842 KT188872
Pristionchus degawai MH114984 –
Pristionchus elegans KJ877238 KJ877274
Pristionchus entomophagus FJ040441 KT188873
Pristionchus exspectatus KT188849 KT188879
Pristionchus fissidentatus KT188855 KJ877273
Pristionchus fukushimae KT188852 KT188882
Pristionchus hongkongensis MH114985 –
Pristionchus hoplostomus KT188853 KT188883
Pristionchus japonicus KT188850 KT188880
Pristionchus laevicollis MH114986 –
Pristionchus lheritieri SB245 KT188846 KT188876
Pristionchus lucani KT188844 KT188874
Pristionchus marianneae FJ040442 KT188866
Pristionchus maupasi FJ040443 KT188863
Pristionchus maxplancki KT188851 KT188881
Pristionchus mayeri KT188835 KT188865
Pristionchus neolucani MH114987 –
Pristionchus occultus KX113518 –
Pristionchus pacificus PS312 AF083010 EU195982
Pristionchus pauli FJ040446 KT188870
Pristionchus paulseni MH114982 –
Pristionchus pseudaerivus FJ040447 KT188864
Pristionchus quartusdecimus KT188847 KT188877
Pristionchus racemosae KT188859 KT188888
Pristionchus riukiariae MH114988 –
Pristionchus sycomori KT188857 KT188886
Pristionchus triformis KT188854 KT188884
Pristionchus uniformis KJ877236 KJ877272
Pristionchus yamagatae MH114983 –
Pristionchus sp. 2 CW-2016 KX113519 –
Pristionchus sp. 3 CW-2016 KX113517 –
Pristionchus sp. 35 VS-2015 KT188858 KT188887
Pristionchus sp. 38 VS-2015 KT188861 KT188890
Pristionchus trametes n. sp. LC633357

Morphometric values for Pristioinchus trametes n. sp.

Male Female
Holotype Paratypes Paratypes
N – 9 10
L 712 696 ± 58 (615–785) 819 ± 41 (746–893)
a 24.1 23.9 ± 1.7 (20.9–26.1) 19.9 ± 0.7 (18.4–20.9)
b 6.3 6.5 ± 0.5 (5.9–7.6) 7.2 ± 0.4 (6.7–7.6)
c 12.4 11.9 ± 0.6 (11.1–13.3) 12.3 ± 1.2 (10.2–13.9)
c′ 2.4 2.4 ± 0.1 (2.2–2.6) 3.6 ± 0.5 (2.8–4.4)
T or V 78.0 84.5 ± 3.3 (77.6–88.9) 51.6 ± 0.8 (50.4–52.8)
Anterior pharynx 59 55 ± 2.8 (49–59) 57 ± 3.0 (54–64)
Posterior pharynx 49 48 ± 2.2 (43–52) 51 ± 3.1 (45–55)
Anterior/posterior pharynx ratio 1.1 1.1 ± 0.1 (1.0–1.2) 1.1 ± 0.1 (1.0–1.2)
Nerve ring from anterior end 79 75 ± 3.9 (68–81) 77 ± 4.0 (72–85)
Secretory-excretory pore from anterior end 102 99 ± 5.0 (93–110) 104 ± 5.0 (96–112)
Deirid from anterior end 117 116 ± 4.9 (110–126) 127 ± 5.0 (117–134)
Median bulb diam. 17.6 16.9 ± 0.7 (15.8–18.0) 19.0 ± 0.7 (18.0–19.8)
Basal bulb diam. 14.3 12.9 ± 1.0 (11.0–14.4) 14.3 ± 0.7 (13.3–15.1)
Maximum body diam. 30 29 ± 3.2 (25–36) 41 ± 2.4 (37–43)
Cloacal or anal body diam. 24 24 ± 1.6 (22–27) 19 ± 1.1 (14–22)
Tail length 58 59 ± 3.9 (52–65) 67 ± 7.1 (60–79)
Gonad length 553 588 ± 50 (519–668) –
Vas deferens length 102 110 ± 14 (95–136) –
Ratio of vas deferens to total gonad (%) 18 19 ± 1.3 (17–21)
Spicule (arc) 40 39 ± 2.1 (36–42) –
Spicule (chord) 33 31 ± 1.7 (29–33) –
Gubernaculum (chord) 16.8 17.6 ± 1.4 (15.8–19.9) –
Tail spike length 13.8 12.9 ± 2.1 (9.2–16.8) –
Ratio (%) of tail spike to total tail 24 22 ± 2.9 (16–26) –
Anterior ovary – – 255 ± 17 (229–278)
Posterior ovary – – 286 ± 54 (214–363)
Vulval body diam. – – 41 ± 2.1 (37–43)
Anus-phasmid – – 38 ± 4.1 (34–48)
Relative position of phasmid to total tail (%) – – 57 ± 5.6 (48–66)
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