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Figure 1:

Galls caused by Meloidogyne incognita on the root of Calendula officinalis L.
Galls caused by Meloidogyne incognita on the root of Calendula officinalis L.

Figure 2:

Meloidogyne incognita (Kofoid & White, 1919) from Calendula officinalis A: Anterior end region, B: Tail region, C-D: Perineal pattern (Scale bar: 20 µm).
Meloidogyne incognita (Kofoid & White, 1919) from Calendula officinalis A: Anterior end region, B: Tail region, C-D: Perineal pattern (Scale bar: 20 µm).

Figure 3:

Amplified DNA of using Meloidogyne incognita-specific primers A: MincF1/MincR1 primer set, B: Inc-K14F/Inc-K14R primer set. M: DNA Ladder, Hibrigen 100 bp; BA1-BA5: Samples; S6: M. incognita (positive control); W: Water.
Amplified DNA of using Meloidogyne incognita-specific primers A: MincF1/MincR1 primer set, B: Inc-K14F/Inc-K14R primer set. M: DNA Ladder, Hibrigen 100 bp; BA1-BA5: Samples; S6: M. incognita (positive control); W: Water.

Morphometric characters of second-stage juveniles (J2) of Meloidogyne incognita on Calendula officinalis.

Diagnostic characters This study Whitehead (1968) Eisenback and Triantaphyllou (1991)
Body length 399.79 ± 8.09 (389.27–418.95) 360–393 346–463
Body width 13.87 ± 0.86 (12.18–15.28)
Body width at stylet base 8.98 ± 0.33 (7.96–9.60)
Body width at anus 9.99 ± 0.68 (8.73–11.28)
Stylet length 10.57 ± 0.66 (9.13–11.91) 10 10–12
DGO 3.02 ± 0.37 (2.30–3.94) 2–2.5 2–3
Tail length 44.93 ± 3.16 (39.15–52.00) 42–63
Excretory pore to head end 76.78 ± 5.59 (61.67–85.95)
Body width at excretory pore 12.78 ± 0.56 (11.65–14.73)
a 28.91 ± 1.57 (25.94–32.55) 29–33
b 5.47 ± 0.29 (4.92–5.92)
c 8.94 ± 0.64 (7.63–10.07) 8–9.4
c’ 4.51 ± 0.37 (3.87–5.31)
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