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Figure 1:
A. Hop roots, Humulus lupulus L. (Mantiqueira cultivar), with galls formed by Meloidogyne incognita (Kofoid and White, 1919) Chitwood, 1949; B. Roots with apparent nodules, characterizing the galls; C. Isoenzymatic esterase phenotype (I1 = M. incognita) of females recovered from hops roots Mi.1 and Mi.2; J3 = M. javanica (Treub, 1885) Chitwood, 1949, control. Trapezoidal labial region of male (a), a prominent labial disc in relation to the submedian lips with transverse streaks (b) and the stylet basal knobs height than wide (c); D. Perineal region of a female with high, trapezoidal dorsal arch and thick streaks, typical of M. incognita. São Paulo, Brazil.
Figure 2:
Molecular specific identification of Meloidogyne incognita (Kofoid and White, 1919) Chitwood, 1949, from roots of Humulus lupulus L. (Mi.1; Mi.2, Mi.3); L: 100 bp Plus DNA Ladder (Thermo Scientific); J3: negative control of Meloidogyne javanica (Treub, 1885) Chitwood, 1949, species; CN: negative control of PCR reaction; PC: positive control of M. incognita.