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Figure 1:
Photomicrographs of Vittatidera zeaphila population on Corn (Zea mays) from Spencer County, Indiana. (A, B): light micrograph of vulval cones of V. zeaphila showing fenestra area with arrow showing the anal area, (C, D): males anterior ends showing head and esophageal regions, (E, F): second-stage juveniles anterior ends showing head and esophageal regions, (G, H): second-stage juveniles posterior ends showing tail and tail terminus, (I): male posterior end showing the spicule, (J): lateral field with four lines at mid-body, (K): cysts with attached egg masses on corn roots with arrows showing second-stage juveniles feeding on corn roots.
Figure 2:
Phylogenetic relationships of Vittatidera zeaphila and other selected cyst nematodes, as inferred from a 1887 bp alignment of partial Hsp90 genomic DNA sequences, with Helicotylenchus digonicus as the outgroup. A 50% majority rule consensus tree obtained from Bayesian analysis was generated using the GTR + I + G model of nucleotide substitution. Branch support values above 50% are shown on appropriate branches. New sequences are highlighted in bold.
Figure 3:
Phylogenetic relationships of Vittatidera zeaphila and other select cyst nematodes, as inferred from a 373 bp alignment representing 33 taxa, with Atalodera carolynae as the outgroup. The tree was constructed in IQ-TREE using model K3Pu + F + I + G4; the consensus tree was constructed from 1,000 bootstrap trees using UFBoot2. Bootstrap values are included on appropriate branches. New sequences are highlighted in bold.