Login
Registrieren
Passwort zurücksetzen
Veröffentlichen & Verteilen
Verlagslösungen
Vertriebslösungen
Themen
Allgemein
Altertumswissenschaften
Architektur und Design
Bibliotheks- und Informationswissenschaft, Buchwissenschaft
Biologie
Chemie
Geowissenschaften
Geschichte
Industrielle Chemie
Informatik
Jüdische Studien
Kulturwissenschaften
Kunst
Linguistik und Semiotik
Literaturwissenschaft
Materialwissenschaft
Mathematik
Medizin
Musik
Pharmazie
Philosophie
Physik
Rechtswissenschaften
Sozialwissenschaften
Sport und Freizeit
Technik
Theologie und Religion
Wirtschaftswissenschaften
Veröffentlichungen
Zeitschriften
Bücher
Konferenzberichte
Verlage
Blog
Kontakt
Suche
EUR
USD
GBP
Deutsch
English
Deutsch
Polski
Español
Français
Italiano
Warenkorb
Home
Zeitschriften
Journal of Nematology
Band 51 (2019): Heft 1 (January 2019)
Uneingeschränkter Zugang
Temporal expression patterns of
Pasteuria
spp. sporulation genes
Ruhiyyih Dyrdahl-Young
Ruhiyyih Dyrdahl-Young
,
Weiming Hu
Weiming Hu
und
Peter DiGennaro
Peter DiGennaro
| 29. Juli 2019
Journal of Nematology
Band 51 (2019): Heft 1 (January 2019)
Über diesen Artikel
Vorheriger Artikel
Nächster Artikel
Zusammenfassung
Artikel
Figuren und Tabellen
Referenzen
Autoren
Artikel in dieser Ausgabe
Vorschau
PDF
Zitieren
Teilen
Article Category:
Arts & Humanities
Online veröffentlicht:
29. Juli 2019
Seitenbereich:
1 - 8
Eingereicht:
12. Dez. 2018
DOI:
https://doi.org/10.21307/jofnem-2019-039
© 2019 Ruhiyyih Dyrdahl-Young et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.
Figure 1:
The Bacillus subtillus homologous sporulation gene Spo0F was identified and sequenced in Pasteuria penetrans. Spo0F nucleotide and translated amino acid sequence (A). The Pasteuria spp. sequences are identical and share a 72% identity with the B. subtilis sequence (B). The sequences and positions of primers used in the qPCR gene expression analysis for this gene are noted.
Figure 2:
The conserved RNA sigma factor Sigma-G was identified in Pasteuria penetrans. The nucleotide sequence is 71% similar the Bacillus subtilis sequence (A). The amino acid sequence (B) has 83% identity with the B. subtilis, Sigma-G.
Figure 3:
Nucleotide sequence (A) and amino acid residue (B) of Bacillus subtilis and Pasteuria penetrans sigma factor, Sigma-F.
Figure 4:
To track the lifecycle of the obligate endoparasite Pasteuria penetrans, the timing of the expression of Bacillus subtilis homologous sporulation gene Spo0F (A) and sigma factors Sigma-F (B) and Sigma-G (C) was quantified throughout the 30 d lifecycle using RT-qPCR. The results represent three independent replicates each from individual root systems. Pairwise comparisons using Student’s t-test (α = 0.05) were used to examine differences in the expression levels at all timepoints. *Statistically differences. There were significantly more copies of the transcript Spo0F on day 15 than days 25 or 30. There were also significantly more Sigma-F transcripts on day 20 than on day 25 or 30.
Figure 5:
Light microscopic images of the development of Pasteuria penetrans. Photographs taken 10 (A), 15 (B), 20 (C), and 25 (D) days after Meloidogyne arenaria exposure to P. penetrans. Arrows indicate microcolonies (A), thali (B), nascent sporogonium (C) and sporogonium (D). Scale bar represents 10 µm, two images are shown for each developmental stage.
Figure 6:
The percentage of each sporulation structures was assessed to track the lifecycle of Pasteuria penetrans in its nematode host. For each of the four time points assessed, five independent infected nematodes samples were excised from inoculated roots and the numbers of microcolonies (A), thali (B), nascent sporogonium (C), sporogonium (D), and mature endospores (E) were counted by two observers. Shown is mean observations expressed as a percentage of the total number of sporulation structures identified. Pairwise comparisons using t-test (α = 0.05) was conducted to compare a given time point to the initial time point (15 d).
Vorschau