Uneingeschränkter Zugang

Description of Geocenamus vietnamensis sp. n. (Nematoda: Merliniidae) from Vietnam

Huu Tien Nguyen
Huu Tien Nguyen
, 
Thi Mai Linh Le
Thi Mai Linh Le
, 
Thi Duyen Nguyen
Thi Duyen Nguyen
, 
Gracia Liebanas
Gracia Liebanas
, 
Thi Anh Duong Nguyen
Thi Anh Duong Nguyen
 und 
Quang Phap Trinh
Quang Phap Trinh
   | 27. Mai 2019
Journal of Nematology's Cover Image
Über diesen Artikel
Vorheriger Artikel
Nächster Artikel
Zitieren
Article Category: Arts & Humanities
Online veröffentlicht: 27. Mai 2019
Seitenbereich: 1 - 12
Eingereicht: 04. Dez. 2018
DOI: https://doi.org/10.21307/jofnem-2019-025
© 2019 Huu Tien Nguyen et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1:

Geocenamus vietnamensis n. sp. (♀). (A): Entire body; (C): Pharyngeal region; €: Head region; (F): Lateral field at mid-body; (G): Vulva region with posterior gonad; (H): tail region; (K): Vulva region. (♂). (B): Entire body; (D): Pharyngeal region; (I): Tail region; (L): Cloacal region showing spicule and gubernaculum.
Geocenamus vietnamensis n. sp. (♀). (A): Entire body; (C): Pharyngeal region; €: Head region; (F): Lateral field at mid-body; (G): Vulva region with posterior gonad; (H): tail region; (K): Vulva region. (♂). (B): Entire body; (D): Pharyngeal region; (I): Tail region; (L): Cloacal region showing spicule and gubernaculum.

Figure 2:

Geocenamus vietnamensis n. sp. (Light microscope). (A): Entire body of female and male (female in the left side and male in the right side); (B): Head region of the female; (C): Head region of the male; (D): Vulva region with spermatheca at arrow position; (E): Female pharyngeal region; (F): Spicule and Gubernaculum; (G): Phasmid in the tail of the male; (H): Vulva region; (I): Tail of the male; (J): Phasmid in the tail of the female; (K): Lateral field; (L): Tail of the female.
Geocenamus vietnamensis n. sp. (Light microscope). (A): Entire body of female and male (female in the left side and male in the right side); (B): Head region of the female; (C): Head region of the male; (D): Vulva region with spermatheca at arrow position; (E): Female pharyngeal region; (F): Spicule and Gubernaculum; (G): Phasmid in the tail of the male; (H): Vulva region; (I): Tail of the male; (J): Phasmid in the tail of the female; (K): Lateral field; (L): Tail of the female.

Figure 3:

Geocenamus vietnamensis n. sp. (SEM ♀). (A): Labial region; (B): Head region; (C): Enface view with amphid orifice (ap) at arrow position; (D): Vulva region with lateral field at arrow position; €: Secretory–excretory pore (ep); (F): Vulva with epyptigma (ept) at arrow position; (G): anus position (a) and phasmid position (ph); (H): phasmid position; (I): Tail region; (J): Tail tip.
Geocenamus vietnamensis n. sp. (SEM ♀). (A): Labial region; (B): Head region; (C): Enface view with amphid orifice (ap) at arrow position; (D): Vulva region with lateral field at arrow position; €: Secretory–excretory pore (ep); (F): Vulva with epyptigma (ept) at arrow position; (G): anus position (a) and phasmid position (ph); (H): phasmid position; (I): Tail region; (J): Tail tip.

Figure 4:

Geocenamus vietnamensis n. sp. (SEM ♂). (A): Labial region; (B): Head region; (C): Enface view with amphid orifice (ap) at arrow position; (D): Lateral field; (E): Secretory–excretory pore; (F): Tail region with spicule (sp); (G): Tail tip; (H): Side view of tail with spicule (sp) and phasmid (ph) positions; (I): Cloacal region; (I): Cloaca with hypotigma; (J): Phasmid position.
Geocenamus vietnamensis n. sp. (SEM ♂). (A): Labial region; (B): Head region; (C): Enface view with amphid orifice (ap) at arrow position; (D): Lateral field; (E): Secretory–excretory pore; (F): Tail region with spicule (sp); (G): Tail tip; (H): Side view of tail with spicule (sp) and phasmid (ph) positions; (I): Cloacal region; (I): Cloaca with hypotigma; (J): Phasmid position.

Figure 5:

Phylogenetic tree generated from D2–D3 of 28S rDNA sequences based on ML method (TN93 + G model) with 1,000 replications.
Phylogenetic tree generated from D2–D3 of 28S rDNA sequences based on ML method (TN93 + G model) with 1,000 replications.

Figure 6:

Phylogenetic tree generated from ITS rDNA sequences based on ML method (GTR + G model) with 1,000 replications.
Phylogenetic tree generated from ITS rDNA sequences based on ML method (GTR + G model) with 1,000 replications.

Morphometric data of Geocenamus vietnamensis n. sp. from fixed specimens. All measurements are in μ m (except for ratio) and in the form: mean ± s.d. (range).

Paratypes
Geocenamus measurement Holotype female Allotype male Female (n = 18) Male (n = 15)
Body length (L) 877 712 875 ± 64 (776–979) 706 ± 35 (631–754)
Lip region height 3.1 3.6 3.6 ± 0.5 (3.1–4.2) 3.4 ± 0.3 (3.1–3.6)
Lip region width 6.2 5.7 6.5 ± 0.7 (5.2–7.3) 6.1 ± 0.5 (5.2–6.8)
Stylet cone 18.7 15.6 15.7 ± 1.4 (13.5–18.7) 15.1 ± 1.4 (13–17.7)
Stylet shaft 9.4 8.3 10.4 ± 0.5 (9.4–11.4) 8.8 ± 1.6 (5.7–10.9)
Stylet length 28 24 26 ± 1 (24–28) 24 ± 1 (23–26)
Stylet knob height 2.1 1.6 2.0 ± 0.4 (1.6–2.6) 1.9 ± 0.4 (1.6–2.6)
Stylet knob width 4.2 3.1 3.5 ± 0.8 (1.6–4.2) 3.6 ± 0.2 (3.12–3.6)
Stylet base to dorsal gland orifice (DGO) 3.1 3.12 2.9 ± 0.3 (2.6–3.1) 3.0 ± 0.3 (2.6–3.6)
Body width at stylet base 16.6 11.9 16.7 ± 2.8 (13.0–20.8) 13.7 ± 1.0 (11.9–15.6)
Maximum body diameter (MBD) 31 22 29 ± 3 (25–34) 22 ± 1 (22–25)
Body width at vulva 22 27 ± 3 (22–34)
Body width at anus 18.7 13 19.1 ± 2.4 (15.6–23.9) 16.1 ± 2.6 (13–21.8)
Anterior end to the end of pharynx 152 125 138 ± 9 (124–152) 122 ± 7 (114–135)
Anterior end to the end of median bulb 89 73 81 ± 8 (74–99) 72 ± 5 (67–81)
Anterior end to median valve 78 65 72 ± 7 (66–88) 63 ± 5 (57–71)
Median bulb width 10.9 9.8 11.0 ± 0.7 (10.4–12.5) 9.6 ± 1.2 (6.8–10.4)
Median bulb length 15.6 15.6 17.0 ± 1.6 (14.6–18.7) 15.5 ± 1.2 (13.5–17.7)
Anterior end to nerve ring 86 86 90 ± 6 (83–104) 83 ± 8 (74–99)
Anterior end to secretory–excretory pore 94 89 94 ± 5 (86–102) 86 ± 6 (76–97)
Isthmus length 39 31 32 ± 4 (26–39) 30 ± 2 (26–31)
Basal bulb length 27 21 25 ± 2 (23–28) 22 ± 2 (19–25)
Tail length 86 75 104 ± 14 (85–125) 84 ± 9 (68–96)
Spicule length 21 20 ± 1 (19–22)
Gubernaculum length 5.2 6.5 ± 1.0 (5.2–7.8)
Anus to phasmid 23 17.7 23 ± 3 (18.7–26) 18.6 ± 2.6 (13.5–23)
Tail annuli (ventral) 61 48 64 ± 5 (58–73) 59 ± 6 (48–65)
a = L/MBD 28 33 30 ± 3 (25–34) 32 ± 1 (29–33)
b′ = L/distance from anterior end to pharyngo–intestinal valve 5.8 5.7 6.4 ± 0.5 (5.8–7.2) 5.8 ± 0.2 (5.5–6.2)
c = L/Tail length 10.2 9.5 8.5 ± 0.8 (7.3–10.2) 8.5 ± 0.7 (9.3–9.5)
c′ = Tail length/ABD 4.6 5.8 5.5 ± 0.7 (4.3–6.5) 5.3 ± 0.7 (4.3–6.3)
V = Anterior end to vulva/L ×100 50 49 ± 2 (47–52)
M = stylet conus length/stylet length ×100 67 65 60 ± 3 (56–67) 62 ± 4 (57–69)
MB = % distance from anterior end to median bulb in relation to the length of pharynx 51 52 52 ± 3 (49–59) 51 ± 1 (50–53)
O = % distance of dorsal pharyngeal gland opening from stylet knobs in relation to the stylet length 11.1 13.0 11.0 ± 1.3 (9.6–13.0) 12.5 ± 1.3 (10.2–14.3)
Phasmid % tail 27 24 22 ± 2 (18.3–27) 22 ± 3 (17–28)
eISSN:
2640-396X
Sprache:
Englisch
Zeitrahmen der Veröffentlichung:
Volume Open
Fachgebiete der Zeitschrift:
Biologie, andere
Zeitschrift RSS Feed