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Description of Longidorus azarbaijanensis n. sp. (Dorylaimida: Longidoridae) from Iran


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Figure 1

Longidorus azarbaijanensis n. sp. A: pharynx; B: anterior genital branch; C, D: female anterior end; E-G: entire female; H: pharyngeal bulb and the corresponding details; I-L: tail of juveniles from J1 to J4, respectively; M-P: female tail.
Longidorus azarbaijanensis n. sp. A: pharynx; B: anterior genital branch; C, D: female anterior end; E-G: entire female; H: pharyngeal bulb and the corresponding details; I-L: tail of juveniles from J1 to J4, respectively; M-P: female tail.

Figure 2

Longidorus azarbaijanensis n. sp. Female. A: anterior region; B: lip region; C: funnel-shaped amphidial pouch, observed only in one female; D: pharyngeal bulb and the dorsal pharyngeal gland’s nucleus; E: one of ventrosublateral pharyngeal glands’ nucleus; F: part of anterior genital branch; G: vulvar region and part of tubular uterus; H-K: tail of juveniles from J1 to J4, respectively; L-P: female tail. Scale bars=10 μm).
Longidorus azarbaijanensis n. sp. Female. A: anterior region; B: lip region; C: funnel-shaped amphidial pouch, observed only in one female; D: pharyngeal bulb and the dorsal pharyngeal gland’s nucleus; E: one of ventrosublateral pharyngeal glands’ nucleus; F: part of anterior genital branch; G: vulvar region and part of tubular uterus; H-K: tail of juveniles from J1 to J4, respectively; L-P: female tail. Scale bars=10 μm).

Figure 3

Longidorus azarbaijanensis n. sp. Correlation of functional and replacement odontostyle to body length in juveniles and females.
Longidorus azarbaijanensis n. sp. Correlation of functional and replacement odontostyle to body length in juveniles and females.

Figure 4

Bayesian tree inferred under the GTR+G+I model from 28S rDNA D2-D3 expansion domains of Longidorus azarbaijanensis n. sp. Posterior probability and bootstrap values exceeding 50% are given on appropriate clades in the form BPP/BS.
Bayesian tree inferred under the GTR+G+I model from 28S rDNA D2-D3 expansion domains of Longidorus azarbaijanensis n. sp. Posterior probability and bootstrap values exceeding 50% are given on appropriate clades in the form BPP/BS.

Figure 5

Bayesian tree inferred under the GTR+G+I model from ITS1 rDNA partial sequences of Longidorus azarbaijanensis n. sp. Posterior probability and bootstrap values exceeding 50% are given on appropriate clades in the form BPP/BS.
Bayesian tree inferred under the GTR+G+I model from ITS1 rDNA partial sequences of Longidorus azarbaijanensis n. sp. Posterior probability and bootstrap values exceeding 50% are given on appropriate clades in the form BPP/BS.

Morphometrics of Longidorus azarbaijanensis n. sp. All measurements are in μm (except L in mm) and in the form: mean±SD (range).

Juvenile (Paratype) Female
Character J1 J2 J3 J4 Holotype Paratypes
n 1 2 8 3 13
L (mm) 3.2±0.2 4.5±0.1 5.4 6.0±0.5
1.6 2.3, 2.4 (3.0-3.6) (4.4-4.6) (5.4-6.8)
a 127.2±7.1 148.5±14.7 181.5 166.6±13.4
95.1 114.5, 99.7 (116.5-138.3) (135.7-164.6) (133.7-181.5)
b 9.0±1.3 10.5±0.6 14.6 15.1±1.7
6.9 6.5, 6.5 (7.7-11.4) (9.9-11.1) (12.1-17.3)
c 83.9±10.1 128.2±5.2 147.2 178.9±21.4
43.7 58.7, 56.8 (74.1-101.7) (122.5-132.8) (147.2-216.3)
c' 2.1±0.3 1.6±0.1 1.7 1.4±0.2
3.1 2.6, 2.6 (1.5-2.4) (1.5-1.8) (1.2-1.7)
V% 56 54.7±1.5
(52.0-56.4)
Lip region width 10.4±0.6 11.3±0.6 11.5 11.7±0.7
8 10, 10 (9.5-11.0) (11-12) (10.0-12.5)
Lip region height 8 6.8±1.1
(5-8)
Odontostyle length 61.3±2.3 68.8±1.9 77 75.6±2.2
52 57, 56 (58.0-64.5) (67.5-71.0) (73-81)
Odontophore length 45.0±3.4 47.5±5.9 44 47.5±1.7
31 41, 41 (42-52) (42.5-54.0) (44-50)
Replacement odontostyle 69.2±2.0 78.7±2.5
58.5 60, 64 (66.5-73.0) (76-81)
Stylet total length 106.1±5.0 116.3±6.0 121 123.0±2.5
83 97.5, 97.5 (99.0-116.5) (110-122) (120-129)
Anterior end to guiding ring 23 25.0±1.2
(23-27)
Pharynx length 357.5±33.0 430.0±15.6 372.5 401.2±28.2
235 350, 377 (311-405) (417.5-447.5) (372.5-468.8)
Pharyngeal bulb length 98 103.1±5.8
(95.0-113.5)
Pharyngeal bulb width 11 14.8±2.5
(11-21)
Diam. at pharynx base 26.5 29.4±3.8
(26-39)
-at mid-body 25.2±1.4 30.7±2.8 30 36.6±5.4
17 20.0, 24.5 (23-27) (27.5-32.5) (30.0-49.5)
-at anus 18.6±1.5 22.0±1.7 22 25.1±2.6
12 15.0, 16.5 (17-22) (20-23) (22-32)
-at guiding ring level 15 16.1±0.8
(15.0-17.5)
Prerectum length 437.5 331.5±80.4
(240.0-457.5)
Rectum length 20.1±2.4 26.0±1.7 26 27.4±3.6
16 13.5, 17.0 (15-22) (24-27) (21-33)
Lip region-vulva 3051.3 3303.6±249.5
(2881.3-3618.8)
Hyaline part of tail - - - 7 7.8±0.8
(7-9)
Tail 38.4±2.9 35.3±0.6 37 34.0±2.8
37 39, 43 (34-43) (35-36) (30-38)
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