<abstract xmlns="http://www.w3.org/1999/xhtml">

<p><italic>Steinernema carpocapsae</italic> is an entomopathogenic nematode widely studied for its properties as a biocontrol agent in insect pest management and as a model for understanding bacterial symbioses. Less attention has been given to the development of specific anatomical structures within <italic>S. carpocapsae</italic>. A better understanding of entomopathogenic nematode development and anatomy may lead to improved biocontrol efficacy. We recently demonstrated that the neuroanatomy of <italic>S. carpocapsae</italic> IJs differs from the dauer stage of <italic>Caenorhabditis elegans.</italic> Here, we used in vitro cultures of <italic>S. carpocapsae</italic> to examine the early development of the ventral nerve cord (VNC). Similar to <italic>C. elegans, S. carpocapsae</italic> hatches as a J1 with a VNC containing only a fraction of the neurons found in later developmental stages. During J1 development, <italic>S. carpocapsae</italic> adds additional cells to the VNC to establish the complete set of neurons. During our examination of the VNC, we also noted variable gonad arm development among <italic>S. carpocapsae</italic> individuals. Using synchronized in vitro cultures, we found that the gonad migration pattern in <italic>S. carpocapsae</italic> was distinct from both <italic>C. elegans</italic> and the Diplogaster nematode <italic>Pristionchus pacificus.</italic> The <italic>S. carpocapsae</italic> gonad arm migration was highly variable.</p>
</abstract>

Steinernema carpocapsae is an entomopathogenic nematode widely studied for its properties as a biocontrol agent in insect pest management and as a model for understanding bacterial symbioses. Less attention has been given to the development of specific anatomical structures within S. carpocapsae. A better understanding of entomopathogenic nematode development and anatomy may lead to improved biocontrol efficacy. We recently demonstrated that the neuroanatomy of S. carpocapsae IJs differs from the dauer stage of Caenorhabditis elegans. Here, we used in vitro cultures of S. carpocapsae to examine the early development of the ventral nerve cord (VNC). Similar to C. elegans, S. carpocapsae hatches as a J1 with a VNC containing only a fraction of the neurons found in later developmental stages. During J1 development, S. carpocapsae adds additional cells to the VNC to establish the complete set of neurons. During our examination of the VNC, we also noted variable gonad arm development among S. carpocapsae individuals. Using synchronized in vitro cultures, we found that the gonad migration pattern in S. carpocapsae was distinct from both C. elegans and the Diplogaster nematode Pristionchus pacificus. The S. carpocapsae gonad arm migration was highly variable.

Postembryonic Ventral Nerve Cord Development and Gonad Migration in <italic>Steinernema carpocapsae</italic>


Journal of Nematology

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Postembryonic Ventral Nerve Cord Development and Gonad Migration in 


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Postembryonic Ventral Nerve Cord Development and Gonad Migration in

Steinernema carpocapsae is an entomopathogenic nematode widely studied for its properties as a biocontrol agent in insect pest management and as a model for...

Postembryonic Ventral Nerve Cord Development and Gonad Migration in Steinernema carpocapsae

Article Category: research-article

Online veröffentlicht: 31. Mai 2018

Seitenbereich: 27 - 32

Akzeptiert: 29. Aug. 2017

DOI: https://doi.org/10.21307/jofnem-2018-005

Schlüsselwörter
DAPI, DIC, distal tip cell, entomopathogenic nematode, preanal ganglion, retrovesicular ganglion, vulva

© 2018 Hung Xuan Bui et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Postembryonic Ventral Nerve Cord Development and Gonad Migration in Steinernema carpocapsae

Article Category: research-article

Online veröffentlicht: 31. Mai 2018

Seitenbereich: 27 - 32

Akzeptiert: 29. Aug. 2017

DOI: https://doi.org/10.21307/jofnem-2018-005

SchlüsselwörterDAPI, DIC, distal tip cell, entomopathogenic nematode, preanal ganglion, retrovesicular ganglion, vulva

© 2018 Hung Xuan Bui et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Schlüsselwörter
DAPI, DIC, distal tip cell, entomopathogenic nematode, preanal ganglion, retrovesicular ganglion, vulva