Bio-molecule when isolated from its natural ecological condition is subjected to rapid decay. This decay leads to change in polarization of the molecule and the permittivity. This study presents an experimental analysis of the decay pattern of pM concentration of insulin using whispering gallery mode (WGM) dielectric resonator method. Analysis is carried out by comparing the permittivity, relaxation time and time delay for three days. It is observed that different pM concentrations of insulin solutions start to decay after 24 hours at 50C.