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Poly-3-Hydroxybutyrate As An Example Of A Biopolymer Produced By Methanotrophic Bacteria

Adam Kubaczyński
Adam Kubaczyński
, 
Anna Pytlak
Anna Pytlak
 und 
Zofia Stępniewska
Zofia Stępniewska
   | 05. Okt. 2019
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Article Category: article
Online veröffentlicht: 05. Okt. 2019
Seitenbereich: 329 - 338
Eingereicht: 01. Juli 2018
Akzeptiert: 01. Juli 2019
DOI: https://doi.org/10.21307/PM-2019.58.3.329
© 2019 Adam Kubaczyński et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Methane oxidation and carbon assimilation pathways conducted by type I and II methanothrophs. Explanations: pMMO – particulate form of methane monooxygenase; sMMO – cytoplasmic methane monooxygenase; cytC – cytochrome c; MDH – methanol dehydrogenase; FADH – formaldehyde dehydrogenase; FDH – formate dehydrogenase. Based on Zhang et al. [45].
Methane oxidation and carbon assimilation pathways conducted by type I and II methanothrophs. Explanations: pMMO – particulate form of methane monooxygenase; sMMO – cytoplasmic methane monooxygenase; cytC – cytochrome c; MDH – methanol dehydrogenase; FADH – formaldehyde dehydrogenase; FDH – formate dehydrogenase. Based on Zhang et al. [45].

Fig. 2.

The course of the PHB biosynthesis including the enzymes catalysing subsequent reaction steps and the phbCAB operon genes encoding them. Based on Madison et al. [17].
The course of the PHB biosynthesis including the enzymes catalysing subsequent reaction steps and the phbCAB operon genes encoding them. Based on Madison et al. [17].

Fig. 3.

Medical accessories made of PHB copolymers. Explanation of the presented elements: absorbable surgical threads (A), screws and plates stabilising the bones (B), membranes used in dentistry (C), dressing nets without admixtures – on the left and soaked with medicaments – on the right (D). Based on Bonartsev et al. [4].
Medical accessories made of PHB copolymers. Explanation of the presented elements: absorbable surgical threads (A), screws and plates stabilising the bones (B), membranes used in dentistry (C), dressing nets without admixtures – on the left and soaked with medicaments – on the right (D). Based on Bonartsev et al. [4].

Fig. 4.

Fragments of the PHB film after two months of incubation in soil suspension with different nitrate content. Explanation of the presented elements: Undecomposed film – comparative test (A), film decomposed in anaerobic conditions without addition of nitrates (B), degradation in aerobic conditions without addition of nitrates (C), aerobic degradation with addition of nitrates (D). Based on Bonartsev et al. [4].
Fragments of the PHB film after two months of incubation in soil suspension with different nitrate content. Explanation of the presented elements: Undecomposed film – comparative test (A), film decomposed in anaerobic conditions without addition of nitrates (B), degradation in aerobic conditions without addition of nitrates (C), aerobic degradation with addition of nitrates (D). Based on Bonartsev et al. [4].

Fig. 5.

The course of degradation of the PHB films of different molecular weights: 150, 300, 450, 1000 kDa. PHB degradation took place in vivo, the film was stored for 90 days immersed in phosphate buffer (pH = 7.4) at 70°C. Based on Bonartsev et al. [4].
The course of degradation of the PHB films of different molecular weights: 150, 300, 450, 1000 kDa. PHB degradation took place in vivo, the film was stored for 90 days immersed in phosphate buffer (pH = 7.4) at 70°C. Based on Bonartsev et al. [4].

Summary of the PHB polymer properties

Chemical resistanceValueUnit
Acidsmoderate 
Strong alkalispoor 
Alcoholsmoderate 
Oils and greasegood 
Physical properties
Density1.25g/cm3
Resistance to UV radiationgood 
Mechanical properties
Tensile strength40MPa
Modulus of elasticity3.5GPa
Elongation at break10%
Impact strength (impact strength test according to Izod)35–60J/m2
Thermal properties
Melting point170–180°C
Glass transition temperature0–5°C
Electrical properties
Electrical resistance (specific)1016Ohm/cm
Dielectric constant3.0MHz
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Sprachen:
Englisch, Polnisch
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