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The interdisciplinary analysis of sustainability requires an in-depth study of interactions between different economic entities, and identifying investment aspects of developing relations between them. The concept of Quintuple Helix Model allowed to analyze the interactions and mutual influence between universities, industry (business), government, civil society and the natural environment. Within the Quintuple Helix Model, the central role in the development of the economy is given to knowledge and their transfer between the various subsystems of the Model. The higher education subsystem occupies a special place in the Model because of such reasons: 1) within the educational activity, universities train highly qualified personnel for the national economy; 2) within the extracurricular activity, universities develop new green consciousness of the young generation that is crucial for further sustainable development of the national economy; 3) within the research activity, universities develop innovative regenerative technologies and environmental-friendly technologies. Universities provide the generation, transfer and diffusion of sustainable knowledge in society and business. Synergy of higher education (universities), business (industry), government, public (civil society) is able to create a foundation for sustainable development of the national economy, increase the level of environmental protection, reduce the CO2 emissions, ensure rational use of exhaustive resources, create new green products and raise the standard of living of the country’s population.